LOCUS       sequence-177392-        3283 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Position 3 transfer plasmid for pST44 polycistronic plasmid suite; 
            C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as 
            positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc3-DHFRSTR
FEATURES             Location/Qualifiers
     source          1..3283
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             89..111
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             614..637
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(715..733)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      729..776
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        841..932
                     /gene="bla"
                     /label=AmpR promoter
     terminator      1797..1836
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      1867..2455
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2356..2375
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2609..2626
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2641..2780
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2706..2725)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2866..2888
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2882..3073)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ggcaccatgc atcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt
       61 tccctccggc tccggagagc tcaataattt tgtttaactt taagaaggag atatacatat
      121 gggatccatc agtctgattg cggcgctagc ggtagatcgc gttatcggca tggaaaacgc
      181 catgccatgg aacctgcctg ccgatctcgc ctggtttaaa cgcaacacct taaataaacc
      241 cgtgattatg gggcgccata cctgggaatc aatcggtagg cctttgcccg gccgcaaaaa
      301 tattatcctc agcagtcaac ccgggaccga tgatcgggtt acctgggtta aatcggtcga
      361 cgaagccatc gcggcgtgtg gtgacgtacc agaaatcatg gtgattggcg gcggacgcgt
      421 ttatgaacag ttcttgccaa aagcgcaaaa actgtatctg acgcatatcg atgcagaagt
      481 ggaaggcgac acccattttc cggattacga gccggatgac tgggaatcgg tattcagcga
      541 attccacgat gctgatgcgc agaactcgca tagctattgt ttcgaaatcc tcgagcgtcg
      601 tgccggcagc gcttggagcc acccgcagtt cgaaaaataa tgtacaggta ccacgcgtgc
      661 gcgctgatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
      721 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
      781 aaggaggaac tatatcggga ttagcccgcc taatgagcgg gctttttttt aattccccta
      841 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
      901 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
      961 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctcgtga
     1021 aagtaaaaga cgcagaggac caattggggg cacgagtggg atacatagaa ctggacttga
     1081 atagcggtaa aatccttgag agttttcgcc ctgaagagcg ttttccaatg atgagcactt
     1141 tcaaagttct gctatgtgga gcagtattat cccgtgtaga tgcggggcaa gagcaactcg
     1201 gacgacgaat acactattcg cagaatgact tggttgaata ctccccagtg acagaaaagc
     1261 accttacgga cggaatgacg gtaagagaat tatgtagtgc cgccataacg atgagtgata
     1321 acactgcggc gaacttactt ctgacaacca tcggtggacc gaaggaatta accgcttttt
     1381 tgcacaatat gggagaccat gtaactcgcc ttgaccgttg ggaaccagaa ctgaatgaag
     1441 ccataccaaa cgacgagcga gacaccacaa tgcctgcggc aatggcaaca acattacgca
     1501 aactattaac tggcgaacta cttactctgg cttcacggca acaattaata gactggcttg
     1561 aagcggataa agttgcagga ccactactgc gttcggcact tcctgctggc tggtttattg
     1621 ctgataaatc tggggcagga gagcgtggtt cacggggtat cattgccgca cttggaccag
     1681 atggtaagcc ttcccgtatc gtagttatct acacgacggg tagtcaggca actatggacg
     1741 aacgaaatag acagattgct gaaatagggg cttcactgat taagcattgg taaaccgata
     1801 caattaaagg ctccttttgg agcctttttt tttggacgga ccggtagaaa agatcaaagg
     1861 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1921 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1981 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     2041 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     2101 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     2161 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2221 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     2281 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2341 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2401 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2461 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     2521 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     2581 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     2641 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca atggtgcact
     2701 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac
     2761 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     2821 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     2881 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag
     2941 cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt
     3001 tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt
     3061 cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg taatgatacc
     3121 gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact
     3181 ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac
     3241 tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gtt
//
