LOCUS       sequence-177615-        2808 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  jStack yeast assembly into plant vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Shih PM, Vuu K, Mansoori N, Ayad L, Louie KB, Bowen BP, Northen TR, 
            Loque D
  TITLE     A robust gene-stacking method utilizing yeast assembly for plant 
            synthetic biology.
  JOURNAL   Nat Commun. 2016 Oct 26;7:13215. doi: 10.1038/ncomms13215.
  PUBMED    27782150
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-10-26"; volume: "7:13215. doi"; pages: " 
            10.1038/ncomms13215"
COMMENT     Sequence Label: pms5055(JBEI-10543)
FEATURES             Location/Qualifiers
     source          1..2808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(74..865,866..934))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     697..716
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(935..1039)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1107..1125
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1129..1172)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      complement(1207..1914)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(1952..1969)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2123..2711)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2203..2222)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
       61 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      121 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      181 taccatctgg ccccagtgct gcaatgatac cgcgggaccc acgctcaccg gctccagatt
      241 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
      301 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
      361 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
      421 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
      481 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
      541 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
      601 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
      661 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
      721 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
      781 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
      841 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
      901 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
      961 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1021 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1081 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa
     1141 aaaaatcctt agctttcgct aaggatgatt tctggaattc ggtctcagtc cgaggggatc
     1201 gagcccctgc tgagcctcga catgttgtcg caaaattcgc cctggacccg cccaacgatt
     1261 tgtcgtcact gtcaaggttt gacctgcact tcatttgggg cccacataca ccaaaaaaat
     1321 gctgcataat tctcggggca gcaagtcggt tacccggccg ccgtgctgga ccgggttgaa
     1381 tggtgcccgt aactttcggt agagcggacg gccaatactc aacttcaagg aatctcaccc
     1441 atgcgcgccg gcggggaacc ggagttccct tcagtgagcg ttattagttc gccgctcggt
     1501 gtgtcgtaga tactagcccc tggggcactt ttgaaatttg aataagattt atgtaatcag
     1561 tcttttaggt ttgaccggtt ctgccgcttt ttttaaaatt ggatttgtaa taataaaacg
     1621 caattgtttg ttattgtggc gctctatcat agatgtcgct ataaacctat tcagcacaat
     1681 atattgtttt cattttaata ttgtacatat aagtagtagg gtacaatcag taaattgaac
     1741 ggagaatatt attcataaaa atacgatagt aacgggtgat atattcatta gaatgaaccg
     1801 aaaccggcgg taaggatctg agctacacat gctcaggttt tttacaacgt gcacaacaga
     1861 attgaaagca aatatcatgc gatcataggc gtctcgcata tctcattaaa gcagggagtg
     1921 agaccggatc ctaactcgag ctgcaggctt cctcgctcac tgactcgctg cgctcggtcg
     1981 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     2041 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     2101 aaaaggccgc gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa
     2161 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2221 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     2281 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     2341 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     2401 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     2461 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     2521 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     2581 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     2641 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     2701 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     2761 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttca
//
