LOCUS       sequence-177621-       10731 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Co-expression in S.cerevisiae of 4CL5 and AtSCT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10731)
  AUTHORS   Eudes A, Mouille M, Robinson DS, Benites VT, Wang G, Roux L, Tsai 
            YL, Baidoo EE, Chiu TY, Heazlewood JL, Scheller HV, Mukhopadhyay A, 
            Keasling JD, Deutsch S, Loque D
  TITLE     Exploiting members of the BAHD acyltransferase family to synthesize 
            multiple hydroxycinnamate and benzoate conjugates in yeast.
  JOURNAL   Microb Cell Fact. 2016 Nov 21;15(1):198.
  PUBMED    27871334
REFERENCE   2  (bases 1 to 10731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2016-11-21"; volume: "15(1)"; pages: "198"
COMMENT     Sequence Label: pDRf1-4CL5-AtSCT (JBEI-11557)
FEATURES             Location/Qualifiers
     source          1..10731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      685..1140
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(772..791)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     982..1003
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1170..1186)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1170..1186)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1183..1205)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1194..1210
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1218..1224,1225..1242,1243..1248))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1263..1284
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1401..1418)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1572..2160)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1652..1671)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2331..3122,3123..3191))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2954..2973
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3192..3296)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3364..3382
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3407..3622
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             3623..4426
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      4537..5883
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     primer_bind     6304..6326
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6318..6335
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6319..6335
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(6348..6595)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     6561..6579
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     terminator      complement(8748..8935)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     8856..8880
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     9083..9102
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9083..9101
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    9164..9188
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10584..10608)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aaaatttatc aacgaggttg atagaaaaaa aaagtttttg tagatataga agaaaaaaag
       61 attttcacta ttggtgttat aggaaagaaa gagaaaaaat ttaagagaaa ttagatgtta
      121 gacgataatg ataggacatt tgaaaataat acaatatttg gcctttttat ataatcgaaa
      181 ctaatggagg ggagcataag cggtacccac catcgaccac gtttcgtcac cggtcataat
      241 tggaataaac aaaacaaaca aacgaactaa agagtgtgag agtaacaatt tccacagagg
      301 taaaaaaaaa aaaanggaaa acagaaacaa aaaaagctca tagttctact atagcttctt
      361 cagactttct gtgaacgagg gaaaaagaca acaaaaaagc aatggataaa cggtctcgat
      421 cgaggcctgg aagtgccgca ttaggaaata cagatgcaat gcgcgcgagt ttacgaaatt
      481 ctcgcaggag tttctccggc catgtgcggc tggttctacg tctcgtgtat gggtttattt
      541 ctgcggtaat tatcgccgga tctccgcttc gagaccgggt ttgttttcaa attaccggaa
      601 aaggtcaggc aaaacagagt gatgtatggg tgatcccagc aatgcctgtt tatgtttctc
      661 cgtttcagga agcttggcga atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
      721 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
      781 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
      841 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
      901 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
      961 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     1021 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     1081 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     1141 cctgatgcgg taagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     1201 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     1261 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     1321 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     1381 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     1441 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     1501 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     1561 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     1621 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     1681 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     1741 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     1801 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     1861 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     1921 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     1981 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     2041 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     2101 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     2161 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     2221 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     2281 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     2341 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     2401 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     2461 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     2521 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     2581 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     2641 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     2701 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     2761 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     2821 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     2881 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     2941 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     3001 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     3061 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     3121 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     3181 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     3241 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     3301 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     3361 aaaaataggc gtatcacgag gccctttcgt cttcaagaat tagcttttca attcatcatt
     3421 ttttttttat tctttttttt gatttcggtt tccttgaaat ttttttgatt cggtaatctc
     3481 cgaacagaag gaagaacgaa ggaaggagca cagacttaga ttggtatata tacgcatatg
     3541 tagtgttgaa gaaacatgaa attgcccagt attcttaacc caactgcaca gaacaaaaac
     3601 atgcaggaaa cgaagataaa tcatgtcgaa agctacatat aaggaacgtg ctgctactca
     3661 tcctagtcct gttgctgcca agctatttaa tatcatgcac gaaaagcaaa caaacttgtg
     3721 tgcttcattg gatgttcgta ccaccaagga attactggag ttagttgaag cattaggtcc
     3781 caaaatttgt ttactaaaaa cacatgtgga tatcttgact gatttttcca tggagggcac
     3841 agttaagccg ctaaaggcat tatccgccaa gtacaatttt ttactcttcg aagacagaaa
     3901 atttgctgac attggtaata cagtcaaatt gcagtactct gcgggtgtat acagaatagc
     3961 agaatgggca gacattacga atgcacacgg tgtggtgggc ccaggtattg ttagcggttt
     4021 gaagcaggcg gcagaagaag taacaaagga acctagaggc cttttgatgt tagcagaatt
     4081 gtcatgcaag ggctccctat ctactggaga atatactaag ggtactgttg acattgcgaa
     4141 gagcgacaaa gattttgtta tcggctttat tgctcaaaga gacatgggtg gaagagatga
     4201 aggttacgat tggttgatta tgacacccgg tgtgggttta gatgacaagg gagacgcatt
     4261 gggtcaacag tatagaaccg tggatgatgt ggtctctaca ggatctgaca ttattattgt
     4321 tggaagagga ctatttgcaa agggaaggga tgctaaggta gagggtgaac gttacagaaa
     4381 agcaggctgg gaagcatatt tgagaagatg cggccagcaa aactaaaaaa ctgtattata
     4441 agtaaatgca tgtatactaa actcacaaat tagagcttca atttaattat atcagttatt
     4501 acccaattct catgtttgac agcttatcat cggatcgatc caatatcaaa ggaaatgata
     4561 gcattgaagg atgagactaa tccaattgag gagtggcagc atatagaaca gctaaagggt
     4621 agtgctgaag gaagcatacg ataccccgca tggaatggga taatatcaca ggaggtacta
     4681 gactaccttt catcctacat aaatagacgc atataagtac gcatttaagc ataaacacgc
     4741 actatgccgt tcttctcatg tatatatata tacaggcaac acgcagatat aggtgcgacg
     4801 tgaacagtga gctgtatgtg cgcagctcgc gttgcatttt cggaagcgct cgttttcgga
     4861 aacgctttga agttcctatt ccgaagttcc tattctctag ctagaaagta taggaacttc
     4921 agagcgcttt tgaaaaccaa aagcgctctg aagacgcact ttcaaaaaac caaaaacgca
     4981 ccggactgta acgagctact aaaatattgc gaataccgct tccacaaaca ttgctcaaaa
     5041 gtatctcttt gctatatatc tctgtgctat atccctatat aacctaccca tccacctttc
     5101 gctccttgaa cttgcatcta aactcgacct ctacattttt tatgtttatc tctagtatta
     5161 ctctttagac aaaaaaattg tagtaagaac tattcataga gtgaatcgaa aacaatacga
     5221 aaatgtaaac atttcctata cgtagtatat agagacaaaa tagaagaaac cgttcataat
     5281 tttctgacca atgaagaatc atcaacgcta tcactttctg ttcacaaagt atgcgcaatc
     5341 cacatcggta tagaatataa tcggggatgc ctttatcttg aaaaaatgca cccgcagctt
     5401 cgctagtaat cagtaaacgc gggaagtgga gtcaggcttt ttttatggaa gagaaaatag
     5461 acaccaaagt agccttcttc taaccttaac ggacctacag tgcaaaaagt tatcaagaga
     5521 ctgcattata gagcgcacaa aggagaaaaa aagtaatcta agatgctttg ttagaaaaat
     5581 agcgctctcg ggatgcattt ttgtagaaca aaaaagaagt atagattctt tgttggtaaa
     5641 atagcgctct cgcgttgcat ttctgttctg taaaaatgca gctcagattc tttgtttgaa
     5701 aaattagcgc tctcgcgttg catttttgtt ttacaaaaat gaagcacaga ttcttcgttg
     5761 gtaaaatagc gctttcgcgt tgcatttctg ttctgtaaaa atgcagctca gattctttgt
     5821 ttgaaaaatt agcgctctcg cgttgcattt ttgttctaca aaatgaagca cagatgcttc
     5881 gttaacaaag atatgctatt gaagtgcaag atggaaacgc agaaaatgaa ccggggatgc
     5941 gacgtgcaag attacctatg caatagatgc aatagtttct ccaggaaccg aaatacatac
     6001 attgtcttcc gtaaagcgct agactatata ttattataca ggttcaaata tactatctgt
     6061 ttcagggaaa actcccaggt tcggatgttc aaaattcaat gatgggtaac aagtacgatc
     6121 cgattatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     6181 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     6241 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     6301 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ggcatgcgca aattaaagcc
     6361 ttcgagcgtc ccaaaacctt ctcaagcaag gttttcagta taatgttaca tgcgtacacg
     6421 cgtctgtaca gaaaaaaaag aaaaatttga aatataaata acgttcttaa tactaacata
     6481 actataaaaa aataaatagg gacctagact tcaggttgtc taactccttc cttttcggtt
     6541 agagcggatg tggggggagg gcgtgaatgt aagcgtgaca taactaatta catgactcga
     6601 gcggccgcct atttagagca catggtttcc aatttagctc gcagatcctt cctcagaatc
     6661 ttgcccgaaa ctgcttttgg tataacttct atgaaaaaca ccatcttaat ccgcttgtag
     6721 tgaaccacct gtttgtttac gtaactcttg acatcatctt cagttaactg agacccttga
     6781 gatcttgcta caaatgctac tggaacctca tcagctactt catccttcat tgcaacaact
     6841 gcggcatcat cgatagaagg atgagaaata agcaatgctt caagctcagc tggagccact
     6901 tggtagcctt tgaatttgat cagttccttc aaccgatcaa caatgaagat ctcatcatca
     6961 tcatccacaa acccaatatc tcctgtatgt aaccacccgt ctttgtctat ggttcgtgca
     7021 gtagcttccg gatcattcaa ataacctttc atgagctgat gacctcggac gcagatctcg
     7081 ccagatttgt tgcgcggcaa ggagattccg gtttcggtat cgaccacttt catctcggcg
     7141 tttctgatca cagtcccaca tgcaccggac ttggttttaa atgggttctt tgcaaacgct
     7201 aatgacttag ctaccgttcc ggactcggtc attccataac cctgaccaaa aatggcatta
     7261 ggaaacttga gtcgcacggc gtcttcaagc tccttcttta gtgtagctgc acctgatagc
     7321 ataattctca ccgagctcaa gtcgtatctc tccgtctcag gggatttaat gaatgctaaa
     7381 accaccggag gagccaccgg cacaaccgtg actttgtacc tttgaatcag ctccatcacc
     7441 agattcaact cgaacctcgg cacgatcagt aacgctgcac cggttctcat cgccgaaagc
     7501 atcaacgcgt cgagagcgta aatgtgaaac attgggagaa aacagaggat gacgtcattg
     7561 gccgtgaagt taaggttagg gttttctccg tcgactttct gagcgatgct tgtaactaaa
     7621 cccttgtgag taatcatcac tccctttgga agtcccgttg ttccggaaga gtacggcatc
     7681 gccaccgtgt cttccggcga tattttaggt tttagaagtt ctgtttcgtc cgcttgagtc
     7741 agttccgtga aactcacgca accatcatca ctcgacgaaa caacgccgtt gtcaccgtca
     7801 tcgtctaggc aaacgatcaa aacgccgtcg tttttgaggt ttgttagttt atcgactaaa
     7861 catttcttcg tgatgatcat cttcgcggcg gaggctttag cctgtttagc gatctccggc
     7921 tgagtgtaga acggattcgc ggtagtggaa acggctccaa ggtaagcaac ggcaagaaaa
     7981 gacagagcaa actccggcga gttcgggagg agaagcatta cgacgtctcc gtggcggatc
     8041 ccgagccggt ggattccggc agctatcctc cgcatgttgg tttgtacatc ggcgtaggtc
     8101 aaaatacggc cagtggcacc gtcgatgata caggttgttg aggaatctcc gtcgccatcg
     8161 ccggagaacc tttggaagac gtaatcggtg agagggaggt ggttagggat gaagatatca
     8221 ggaagcttag atcggaaaat gaaatcatga gaaggttctt cttcttcgtc ttcttgatct
     8281 atcttctttg tgagaaaatg cgtttgttgt tggagcacca tgcggccgcc cgggggatcc
     8341 actagaactt tttgattaaa attaaaaaaa ctttttgttt ttgtgtttat tctttgttct
     8401 tagaaaagac aagttgagct tgtttgttct tgatgtttta ttattttaca atagctgcaa
     8461 atgaagaata gattcgaaca ttgtgaagta ttggcatata tcgtctctat ttatactttt
     8521 tttttttcag ttctagtata ttttgtattt tcctcctttt cattctttca gttgccaata
     8581 agttacaggg gatctcgaaa gatggtgggg atttttcctt gaaagacgac tttttgccat
     8641 ctaatttttc cttgttgcct ctgaaaatta tccagcagaa gcaaatgtaa aagatgaacc
     8701 tcagaagaac acgcaggggc ccgaaattgt tcctacgagc tcgcatgccg gtagaggtgt
     8761 ggtcaataag agcgacctca tgctatacct gagaaagcaa cctgacctac aggaaagagt
     8821 tactcaagaa taagaatttt cgttttaaaa cctaagagtc actttaaaat ttgtatacac
     8881 ttattttttt tataacttat ttaataataa aaatcataaa tcataagaaa ttcgcttatt
     8941 tagaagtgtc aacaacgtat ctaccaacga tttgaccctt ttccatcttt tcgtaaattt
     9001 ctggcaaggt agacaagccg acaaccttga ttggagactt gaccaaacct ctggcgaaga
     9061 agtccaaagc tggatccgcg cgtaatacga ctcactatag ggcgaattgg gtaccgggcc
     9121 ccccctcgag gtcgacctgc agcggccatc atgaattaac tacaccactt tgtacaagaa
     9181 agctgggtcc acttagttgg tatctccgaa gtataatttc ttcatagttg ccatttccct
     9241 tttgaattcc accatggcat ctctaggcaa cttcataata accttgacac caccttccat
     9301 agatggatca gattttgatg gtttcaagat cataccaaca tggacagtgg attcacgttg
     9361 tgtcaatggt cttaaattca caggctcgtt ccatccgaaa tccatagaac cgaaccaacc
     9421 gatgttacgc cagtcggtca aaaagaacaa accatcagaa acaccctcaa acattgagtc
     9481 atctctcatc aacctttcag tattcttcaa ctcctcttcg atataacctt tttcataggc
     9541 tgtttttttg gctttcttaa ctctgttggc gatatttgaa atagatgact cttccaactc
     9601 tctaacggtc aattcaacgt agacatcgat gtaagcgttt ccataataac cttttggtaa
     9661 aggtggatct aaaacgtgtc taatacccac ggcgacaccc aagacggtaa taccatcagg
     9721 atttaacttt aaagcacgac ttcttaattt ccagatataa gaagacaaaa cttcgtaagt
     9781 ggtgaatgac tcctttaagt attcacactc cttcatcatt gaatctttta aacgtttaat
     9841 gtcaccagct ctgatattaa tggtttcagt gacaacatcg gtggtaggca aatatgcact
     9901 agtagccaag aaaccgtcga tgtgggaacc agggacctta ccaggtttat tatcaatctt
     9961 accaactaat ctttctcttt gccagacact cttgacggaa ggctctgtct taccagctgc
    10021 taactcggtc aaggcgtgga tgatttgagc aacaccataa ccatcacaga ctgcatgggt
    10081 taaagcggta ccgatagtaa atccaccaca ggcaaactta gtaacttgta aggccaatgg
    10141 gtggtagcag acattagact cataatcaat ttcaatctca ggaactaacc ttaaggcaac
    10201 ttgatcatcc aaattctcaa tgtaatttaa tgaagataag tccaaagtgg aggtggcgac
    10261 ttcgaatggc actccctcac ctaaatagac caattgtaac ttaccgttag attcagaacg
    10321 catcaattta cctgacaaag ggtagtaatg cactaaaagc tcactcaatg ccttacgtaa
    10381 caaactcact ggatcatcta aattctttcc attggcctta aaaacgtaga tagtagcgta
    10441 cataacctca ttgaaagggt cgttatccaa ggtagataat gacaaagttt cacaatgggt
    10501 atgtttagaa ggcttgacta attcaacagg tttcttttca agcaataaag gcaagattgg
    10561 tttcctttga ttagccatgt ggaagcctgc ttttttgtac aaacttgtga tggccgcgaa
    10621 ttcactagtc gaggctgggg tatatttttt ttctttcttt tgaatgtgtg tataaaagag
    10681 agaaaaaatg gttattttcc aattaattaa ttattaacga ttgttaaaga a
//
