LOCUS       sequence-177802-       10232 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express 3x Flagged yki in Drosophila..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10232)
  AUTHORS   Oh H, Irvine KD
  TITLE     In vivo regulation of Yorkie phosphorylation and localization.
  JOURNAL   Development. 2008 Mar;135(6):1081-8. doi: 10.1242/dev.015255. Epub 
            2008 Feb 6.
  PUBMED    18256197
REFERENCE   2  (bases 1 to 10232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.015255"; journalName: "Development"; date: "2008-03"; 
            volume: "135"; issue: "6"; pages: "1081-8"
COMMENT     Sequence Label: pUAST-yki:FLAG(3x)
FEATURES             Location/Qualifiers
     source          1..10232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..247
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     194..215
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             1563..1628
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     intron          1718..1783
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1913..1933
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2205..2339
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2242..2261)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2296..2315
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2366..2384)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2836..2907,3309..3582,3657..4311,4373..4688,4892..5023,
                     5094..5708)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(6487..7041,7042..7072)
                     /label=P element 5' end
     rep_origin      complement(7307..7895)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7387..7406)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8066..8857,8858..8926))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8689..8708
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8927..9031)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9099..9117
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9155..9177)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9277..9296
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(9834..9864,9865..10066)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    10128..10222
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
ORIGIN
        1 agcgagcgcc ggagtataaa tagaggcgct tcgtctacgg agcgacaatt caattcaaac
       61 aagcaaagtg aacacgtcgc taagcgaaag ctaagcaaat aaacaagcgc agctgaacaa
      121 gctaaacaat ctgcagtaaa gtgcaagtta aagtgaatca attaaaagta accagcaacc
      181 aagtaaatca actgcaacta ctgaaatctg ccaagaagta attattgaat acaagaagag
      241 aactctgaat agggaattgg gaattcgtta acagatctgc ggccgcggct cgaatgtgcg
      301 cgtgcctaat cgctaagata attctatgta gttttcgttt gtatacaata agtgcctttt
      361 atatgttaac gacgatgtca gccagcagca atacaaacag cctgatcgag aaggagatcg
      421 acgacgagga catgctttcg ccgatcaagt ccaacaacct ggtggtgcgg gtcaaccagg
      481 acacggacga caacctgcag gcgctattcg acagcgtcct gaatccgggt gacgccaagc
      541 gcccgctgca gctgcccctg cgcatgcgga agctgcccaa ctccttcttc acgcccccgg
      601 cgccctcgca ctcgcgggcc aacagcgccg actccaccta cgacgcgggc tcccagtcga
      661 gcatcaacat cgggaacaag gcgtccatcg tccagcagcc agatggccag tcgcccatcg
      721 ccgccatccc ccagctccag attcagccgt ctccccagca cagccgcctg gcgatacatc
      781 actcccgagc ccgcagcagc cccgcctcgc tgcagcagaa ctacaatgtg cgcgcccgga
      841 gcgacgcagc agcagccaac aatccgaatg ccaatccgag cagccaacag cagcccgctg
      901 ggcccacttt cccagagaac agtgcccaag agttccccag cggcgccccg gccagctcgg
      961 ccattgatct ggacgccatg aacacctgca tgtcgcagga cattcccatg tccatgcaga
     1021 cagtgcacaa gaagcagcgc tcctacgacg tcatcagccc cattcagttg aaccgccaac
     1081 taggcgcctt gccgccggga tgggagcaag ccaagaccaa tgatggccag atctactact
     1141 tgaatcatac tacaaaatct acgcagtggg aggatcccag aatccaatat cgccagcagc
     1201 agcaaatctt gatggccgag cgaataaagc agaatgatgt tttgcaaact acaaaacaaa
     1261 ctaccacatc gaccattgct aacaatttgg gtccactgcc ggatggttgg gagcaggcag
     1321 ttaccgagtc cggagatctt tactttataa atcacattga tcgaacgact tcatggaatg
     1381 atcccagaat gcaatctggg cttagcgtgc tcgactgccc agataactta gtgtcttccc
     1441 tccagattga ggataatctt tgcagtaact tgttcaatga cgcacaggcc attgtaaatc
     1501 cgccgtcttc ccacaaacct gacgatttgg aatggtataa aattaatttc gagcggacca
     1561 tggactacaa agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg
     1621 atgacaaggg taccactaga ggatctttgt gaaggaacct tacttctgtg gtgtgacata
     1681 attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt
     1741 ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac
     1801 tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga
     1861 aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa
     1921 gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca
     1981 tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc
     2041 tgcactgcta tacaagaaaa ttatggaaaa atatttgatg tatagtgcct tgactagaga
     2101 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     2161 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     2221 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     2281 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc
     2341 actagaaggc ctaattctag tatgtatgta agttaataaa acccattttt gcggaaagta
     2401 gataaaaaaa acattttttt tttttactgc actggatatc attgaactta tctgatcagt
     2461 tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat tacctctcac
     2521 tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc cacagaaata
     2581 tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt agcgttacgt
     2641 agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa gctccaagcg
     2701 gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct tcttattttt
     2761 gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca ggggaaagtg
     2821 tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg aggcagcaaa
     2881 cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat cttaagttca
     2941 cttgatctat aggaactgcg attgcaacat caaattgtct gcggcgtgag aactgcgacc
     3001 cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag attattctgg
     3061 tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag acatctaaag
     3121 gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat attttaagct
     3181 gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga tacttcgggg
     3241 tactttttgt tcggggttag atgagcataa cgcttgtagt tgatatttga gatcccctat
     3301 cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc ttcgggcagg
     3361 ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt tcagggagcg
     3421 gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt ggggcggtca
     3481 atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta ttctgcaacg
     3541 agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt ctattcgcag
     3601 tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa actcagtttg
     3661 cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg ccggaaagac
     3721 gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat cgccatccgg
     3781 gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca ggtgcgccta
     3841 tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc tgattttcca
     3901 agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc gcgtggatca
     3961 ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg tgcccggcag
     4021 ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg aggctctaac
     4081 cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct ttaccgccca
     4141 cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca tcctgaccat
     4201 tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga tggccgaggg
     4261 cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct agtgagttcg
     4321 atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc agcgtgggtg
     4381 cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg gccgttgtgc
     4441 ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat tttgccatta
     4501 gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag aagccactgg
     4561 agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc cgggcggtcc
     4621 tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg cgacttattc
     4681 agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat tcttggaaac
     4741 aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt ttttttcaat
     4801 gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact cgcgaatatt
     4861 aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg gcctcatctt
     4921 tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca tcttcctctt
     4981 cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc atgtttagaa
     5041 tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt taggtgttca
     5101 cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc tgtgacacat
     5161 actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca ctggtcttca
     5221 cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc ttcaactgcc
     5281 tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta atatcctgcg
     5341 ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata ccattcctgc
     5401 tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa tggttgtcgt
     5461 acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg gcggacgtgg
     5521 agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg ggcaaggtca
     5581 tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg ggtctggcca
     5641 ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg gcccgacgca
     5701 aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc attattacca
     5761 tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg tgccaataaa
     5821 aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca caagtagact
     5881 ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc aaaaactcgt
     5941 ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca aatcgcgcgc
     6001 cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg ctgtctccca
     6061 cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact gtcgttagtt
     6121 ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca atagaaatta
     6181 gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat atttccctca
     6241 cagctatatt tattctaatt taatattatg actttttaag gtaatttttt gtgacctgtt
     6301 cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt tccttgtgta
     6361 gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca aaaataacaa
     6421 ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg aactagaatt
     6481 aattcggctg ctgctctaaa cgacgcattt cgtactccaa agtacgaatt ttttccctca
     6541 agctcttatt ttcattaaac aatgaacagg acctaacgca cagtcacgtt attgtttaca
     6601 taaatgattt tttttactat tcaaacttac tctgtttgtg tactcccact ggtatagcct
     6661 tcttttatct tttctggttc aggctctatc actttactag gtacggcatc tgcgttgagt
     6721 cgcctccttt taaatgtctg accttttgca ggtgcagcct tccactgcga atcattaaag
     6781 tgggtatcac aaatttggga gttttcacca aggctgcacc caaggctctg ctcccacaat
     6841 tttctcttaa tagcacactt cggcacgtga attaatttta ctccagtcac agctttgcag
     6901 caaaatttgc aatatttcat ttttttttat tccacgtaag ggttaatgtt ttcaaaaaaa
     6961 aattcgtccg cacacaacct ttcctctcaa caagcaaacg tgcactgaat ttaagtgtat
     7021 acttcggtaa gcttcggcta tcgacgggac caccttatgt tatttcatca tgggccagac
     7081 ccacgtagtc cagcggcaga tcggcggcgg agaagttaag cgtctccagg atgaccttgc
     7141 ccgaactggg gcacgtggtg ttcgacgatg tgcagctaat ttcgcccggc tccacgtccg
     7201 cccattggtt aatcagcaga ccctcgttgg cgtaacggaa ccatgagagg tacgacaacc
     7261 atttgaggta tactggcacc gagcccgagt tcaagaagaa ggcgtttttc cataggctcc
     7321 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     7381 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     7441 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     7501 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     7561 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     7621 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     7681 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     7741 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     7801 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     7861 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7921 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     7981 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     8041 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     8101 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     8161 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     8221 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     8281 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     8341 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     8401 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     8461 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     8521 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     8581 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     8641 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     8701 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     8761 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     8821 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     8881 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     8941 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     9001 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     9061 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     9121 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     9181 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     9241 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     9301 gagtgcacca tatgcggtgt gaaataccgc accgaatcgc gcggaactaa cgacagtcgc
     9361 tccaaggtcg tcgaacaaaa ggtgaatgtg ttgcggagag cgggtgggag acagcgaaag
     9421 agcaactacg aaacgtggtg tggtggaggt gaattatgaa gagggcgcgc gatttgaaaa
     9481 gtatgtatat aaaaaatata tcccggtgtt ttatgtagcg ataaacgagt ttttgatgta
     9541 aggtatgcag gtgtgtaagt cttttggtta gaagacaaat ccaaagtcta cttgtgggga
     9601 tgttcgaagg ggaaatactt gtattctata ggtcatatct tgtttttatt ggcacaaata
     9661 taattacatt agctttttga gggggcaata aacagtaaac acgatggtaa taatggtaaa
     9721 aaaaaaaaac aagcagttat ttcggatata tgtcggctac tccttgcgtc gggcccgaag
     9781 tcttagagcc agatatgcga gcacccggaa gctcacgatg agaatggcca gaccatgatg
     9841 aaataacata aggtggtccc gtcggcaaga gacatccact taacgtatgc ttgcaataag
     9901 tgcgagtgaa aggaatagta ttctgagtgt cgtattgagt ctgagtgaga cagcgatatg
     9961 attgttgatt aacccttagc atgtccgtgg ggtttgaatt aactcataat attaattaga
    10021 cgaaattatt tttaaagttt tatttttaat aatttgcgag tacgcaaagc ttggctgcat
    10081 ccaacgcgtt gggagctctc cggatccaag cttgcatgcc tgcaggtcgg agtactgtcc
    10141 tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc
    10201 gagcggagta ctgtcctccg agcggagact ct
//
