LOCUS       sequence-178108-        9944 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic gene circuit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9944)
  AUTHORS   Rubens JR, Selvaggio G, Lu TK
  TITLE     Synthetic mixed-signal computation in living cells.
  JOURNAL   Nat Commun. 2016 Jun 3;7:11658. doi: 10.1038/ncomms11658.
  PUBMED    27255669
REFERENCE   2  (bases 1 to 9944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-06-3"; volume: "7:11658. doi"; pages: " 
            10.1038/ncomms11658"
COMMENT     Sequence Label: Ternary BAC Reporter
FEATURES             Location/Qualifiers
     source          1..9944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(146..164)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     414..433
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      747..833
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(866..1525)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1440..1459
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1526..1628)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      2486..3100
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      2577..3108
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      3176..3395
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             3486..4241
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    4244..4494
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             4820..5995
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             5995..6966
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    7039..7512
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     protein_bind    7577..7634
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attP site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     terminator      complement(7654..7740)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(7843..7863)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     8458..8486
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     RBS             8522..8530
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_RNA        complement(8566..8615)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     protein_bind    8624..8672
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     protein_bind    9796..9829
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     misc_RNA        9843..9892
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             9931..9939
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atggtgagca agggcgaaga agataacatg gccatcatca aggagttcat gcgcttcaag
       61 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      121 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
      181 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
      241 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
      301 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
      361 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
      421 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
      481 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
      541 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
      601 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
      661 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta acccggggga
      721 tcccatggta cgcgtgctag aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
      781 cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgc
      841 cctagataat ggtttcttag acgtcttacg ccccgccctg ccactcatcg cagtactgtt
      901 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
      961 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
     1021 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     1081 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     1141 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     1201 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     1261 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag
     1321 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
     1381 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
     1441 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     1501 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
     1561 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     1621 gccgatcaac gtctcatttt cgccaaaagt tggcccaggg cttcccggta tcaacaggga
     1681 caccaggatt tatttattct gcgaagtgat cttccgtcac aggtatttat tcgcgataag
     1741 ctcatggagc ggcgtaaccg tcgcacagga aggacagaga aagcgcggat ctgggaagtg
     1801 acggacagaa cggtcaggac ctggattggg gaggcggttg ccgccgctgc tgctgacggt
     1861 gtgacgttct ctgttccggt cacaccacat acgttccgcc attcctatgc gatgcacatg
     1921 ctgtatgccg gtataccgct gaaagttctg caaagcctga tgggacataa gtccatcagt
     1981 tcaacggaag tctacacgaa ggtttttgcg ctggatgtgg ctgcccggca ccgggtgcag
     2041 tttgcgatgc cggagtctga tgcggttgcg atgctgaaac aattatcctg agaataaatg
     2101 ccttggcctt tatatggaaa tgtggaactg agtggatatg ctgtttttgt ctgttaaaca
     2161 gagaagctgg ctgttatcca ctgagaagcg aacgaaacag tcgggaaaat ctcccattat
     2221 cgtagagatc cgcattatta atctcaggag cctgtgtagc gtttatagga agtagtgttc
     2281 tgtcatgatg cctgcaagcg gtaacgaaaa cgatttgaat atgccttcag gaacaataga
     2341 aatcttcgtg cggtgttacg ttgaagtgga gcggattatg tcagcaatgg acagaacaac
     2401 ctaatgaaca cagaaccatg atgtggtctg tccttttaca gccagtagtg ctcgccgcag
     2461 tcgagcgaca gggcgaagcc ctcggctggt tgccctcgcc gctgggctgg cggccgtcta
     2521 tggccctgca aacgcgccag aaacgccgtc gaagccgtgt gcgagacacc gcggccggcc
     2581 gccggcgttg tggatacctc gcggaaaact tggccctcac tgacagatga ggggcggacg
     2641 ttgacacttg aggggccgac tcacccggcg cggcgttgac agatgagggg caggctcgat
     2701 ttcggccggc gacgtggagc tggccagcct cgcaaatcgg cgaaaacgcc tgattttacg
     2761 cgagtttccc acagatgatg tggacaagcc tggggataag tgccctgcgg tattgacact
     2821 tgaggggcgc gactactgac agatgagggg cgcgatcctt gacacttgag gggcagagtg
     2881 ctgacagatg aggggcgcac ctattgacat ttgaggggct gtccacaggc agaaaatcca
     2941 gcatttgcaa gggtttccgc ccgtttttcg gccaccgcta acctgtcttt taacctgctt
     3001 ttaaaccaat atttataaac cttgttttta accagggctg cgccctgtgc gcgtgaccgc
     3061 gcacgccgaa ggggggtgcc cccccttctc gaaccctccc ggtcgagtga gcgaggaagc
     3121 accagggaac agcacttata tattctgctt acacacgatg cctgaaaaaa cttcccttgg
     3181 ggttatccac ttatccacgg ggatattttt ataattattt tttttatagt ttttagatct
     3241 tcttttttag agcgccttgt aggcctttat ccatgctggt tctagagaag gtgttgtgac
     3301 aaactgccct ttcagtgtga caaatcaccc tcaaatgaca gtcctgtctg tgacaaattg
     3361 cccttaaccc tgtgacaaat tgccctcaga agaagctgtt ttttcacaaa gttatccctg
     3421 cttattgact cttttttatt tagtgtgaca atctaaaaac ttgtcacact tcacatggat
     3481 ctgtcatggc ggaaacagcg gttatcaatc acaagaaacg taaaaatagc ccgcgaatcg
     3541 tccagtcaaa cgacctcact gaggcggcat atagtctctc ccgggatcaa aaacgtatgc
     3601 tgtatctgtt cgttgaccag atcagaaaat ctgatggcac cctacaggaa catgacggta
     3661 tctgcgagat ccatgttgct aaatatgctg aaatattcgg attgacctct gcggaagcca
     3721 gtaaggatat acggcaggca ttgaagagtt tcgcggggaa ggaagtggtt ttttatcgcc
     3781 ctgaagagga tgccggcgat gaaaaaggct atgaatcttt tccttggttt atcaaacgtg
     3841 cgcacagtcc atccagaggg ctttacagtg tacatatcaa cccatatctc attcccttct
     3901 ttatcgggtt acagaaccgg tttacgcagt ttcggcttag tgaaacaaaa gaaatcacca
     3961 atccgtatgc catgcgttta tacgaatccc tgtgtcagta tcgtaagccg gatggctcag
     4021 gcatcgtctc tctgaaaatc gactggatca tagagcgtta ccagctgcct caaagttacc
     4081 agcgtatgcc tgacttccgc cgccgcttcc tgcaggtctg tgttaatgag atcaacagca
     4141 gaactccaat gcgcctctca tacattgaga aaaagaaagg ccgccagacg actcatatcg
     4201 tattttcctt ccgcgatatc acttccatga cgacaggata gtctgagggt tatctgtcac
     4261 agatttgagg gtggttcgtc acatttgttc tgacctactg agggtaattt gtcacagttt
     4321 tgctgtttcc ttcagcctgc atggattttc tcatactttt tgaactgtaa tttttaagga
     4381 agccaaattt gagggcagtt tgtcacagtt gatttccttc tctttccctt cgtcatgtga
     4441 cttgatatcg ggggttagtt cgtcatcatt gatgagggtt gattatcaca gtttattact
     4501 ctgaattggc tatccgcgtg tgtacctcta cctggagttt ttcccacggt ggatatttct
     4561 tcttgcgctg agcgtaagag ctatctgaca gaacagttct tctttgcttc ctcgccagtt
     4621 cgctcgctat gctcggttac acggctgcgg cgagcgctag tgataataag tgactgaggt
     4681 atgtgctctt cttatctcct tttgtagtgt tgctcttatt ttaaacaact ttgcggtttt
     4741 ttgatgactt tgcgattttg ttgttgcttt gcagtaaatt gcaagattta ataaaaaaac
     4801 gcaaagcaat gattaaagga tgttcagaat gaaactcatg gaaacactta accagtgcat
     4861 aaacgctggt catgaaatga cgaaggctat cgccattgca cagtttaatg atgacagccc
     4921 ggaagcgagg aaaataaccc ggcgctggag aataggtgaa gcagcggatt tagttggggt
     4981 ttcttctcag gctatcagag atgccgagaa agcagggcga ctaccgcacc cggatatgga
     5041 aattcgagga cgggttgagc aacgtgttgg ttatacaatt gaacaaatta atcatatgcg
     5101 tgatgtgttt ggtacgcgat tgcgacgtgc tgaagacgta tttccaccgg tgatcggggt
     5161 tgctgcccat aaaggtggcg tttacaaaac ctcagtttct gttcatcttg ctcaggatct
     5221 ggctctgaag gggctacgtg ttttgctcgt ggaaggtaac gacccccagg gaacagcctc
     5281 aatgtatcac ggatgggtac cagatcttca tattcatgca gaagacactc tcctgccttt
     5341 ctatcttggg gaaaaggacg atgtcactta tgcaataaag cccacttgct ggccggggct
     5401 tgacattatt ccttcctgtc tggctctgca ccgtattgaa actgagttaa tgggcaaatt
     5461 tgatgaaggt aaactgccca ccgatccaca cctgatgctc cgactggcca ttgaaactgt
     5521 tgctcatgac tatgatgtca tagttattga cagcgcgcct aacctgggta tcggcacgat
     5581 taatgtcgta tgtgctgctg atgtgctgat tgttcccacg cctgctgagt tgtttgacta
     5641 cacctccgca ctgcagtttt tcgatatgct tcgtgatctg ctcaagaacg ttgatcttaa
     5701 agggttcgag cctgatgtac gtattttgct taccaaatac agcaatagta atggctctca
     5761 gtccccgtgg atggaggagc aaattcggga tgcctgggga agcatggttc taaaaaatgt
     5821 tgtacgtgaa acggatgaag ttggtaaagg tcagatccgg atgagaactg tttttgaaca
     5881 ggccattgat caacgctctt caactggtgc ctggagaaat gctctttcta tttgggaacc
     5941 tgtctgcaat gaaattttcg atcgtctgat taaaccacgc tgggagatta gataatgaag
     6001 cgtgcgcctg ttattccaaa acatacgctc aatactcaac cggttgaaga tacttcgtta
     6061 tcgacaccag ctgccccgat ggtggattcg ttaattgcgc gcgtaggagt aatggctcgc
     6121 ggtaatgcca ttactttgcc tgtatgtggt cgggatgtga agtttactct tgaagtgctc
     6181 cggggtgata gtgttgagaa gacctctcgg gtatggtcag gtaatgaacg tgaccaggag
     6241 ctgcttactg aggacgcact ggatgatctc atcccttctt ttctactgac tggtcaacag
     6301 acaccggcgt tcggtcgaag agtatctggt gtcatagaaa ttgccgatgg gagtcgccgt
     6361 cgtaaagctg ctgcacttac cgaaagtgat tatcgtgttc tggttggcga gctggatgat
     6421 gagcagatgg ctgcattatc cagattgggt aacgattatc gcccaacaag tgcttatgaa
     6481 cgtggtcagc gttatgcaag ccgattgcag aatgaatttg ctggaaatat ttctgcgctg
     6541 gctgatgcgg aaaatatttc acgtaagatt attacccgct gtatcaacac cgccaaattg
     6601 cctaaatcag ttgttgctct tttttctcac cccggtgaac tatctgcccg gtcaggtgat
     6661 gcacttcaaa aagcctttac agataaagag gaattactta agcagcaggc atctaacctt
     6721 catgagcaga aaaaagctgg ggtgatattt gaagctgaag aagttatcac tcttttaact
     6781 tctgtgctta aaacgtcatc tgcatcaaga actagtttaa gctcacgaca tcagtttgct
     6841 cctggagcga cagtattgta taagggcgat aaaatggtgc ttaacctgga caggtcccgt
     6901 gttccaactg agtgtataga gaaaattgag gccattctta aggaacttga aaagccagca
     6961 ccctgatgcg accacgtttt agtctacgtt tatctgtctt tacttaatgt cctttgttac
     7021 aggccagaaa gcataactgg cctgaatatt ctctctgggc ccactgttcc acttgtatcg
     7081 tcggtctgat aatcagactg ggaccacggt cccactcgta tcgtcggtct gattattagt
     7141 ctgggaccac ggtcccactc gtatcgtcgg tctgattatt agtctgggac cacggtccca
     7201 ctcgtatcgt cggtctgata atcagactgg gaccacggtc ccactcgtat cgtcggtctg
     7261 attattagtc tgggaccatg gtcccactcg tatcgtcggt ctgattatta gtctgggacc
     7321 acggtcccac tcgtatcgtc ggtctgatta ttagtctgga accacggtcc cactcgtatc
     7381 gtcggtctga ttattagtct gggaccacgg tcccactcgt atcgtcggtc tgattattag
     7441 tctgggacca cgatcccact cgtgttgtcg gtctgattat cggtctggga ccacggtccc
     7501 acttgtattg tcgatcagac tatcagcgtg agactacgat tccatcaatg cctgtcaagg
     7561 gcaagtattg acatgtgtcg tggtttgtct ggtcaaccac cgcggtctca gtggtgtacg
     7621 gtacaaaccc cgacatcgat tctagggcgg cggatttgtc ctactcagga gagcgttcac
     7681 cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg
     7741 atgcctctag cacgcgtacc atgggatccc ccgggctgca ggaattcgat atcaagcttt
     7801 tatttgtaga gatcatccat gccatgtgta atcccagcag ctgttacaaa ctcaagaagg
     7861 accatgtggt ctctcttttc gttgggatct ttcgaaaggg cagattgtgt ggacaggtaa
     7921 tggttgtctg gtaaaaggac agggccatcg ccaattggag tattttgttg ataatggtct
     7981 gctagttgaa cgcttccatc ttcaatgttg tgtctaattt tgaagttaac tttgattcca
     8041 ttcttttgtt tgtctgccat gatgtataca ttgtgtgagt tatagttgta ttccaatttg
     8101 tgtccaagaa tgtttccatc ttctttaaaa tcaatacctt ttaactcgat tctattaaca
     8161 agggtatcac cttcaaactt gacttcagca cgtgtcttgt agttcccgtc atctttgaaa
     8221 aatatagttc tttcctgtac ataaccttcg ggcatggcac tcttgaaaaa gtcatgctgt
     8281 ttcatatgat ctgggtatct cgcaaagcat tgaacaccat aaccgaaagt agtgacaagt
     8341 gttggccatg gaacaggtag ttttccagta gtgcaaataa atttaagggt aagttttccg
     8401 tatgttgcat caccttcacc ctctccactg acagaaaatt tgtgcccatt aacatcacca
     8461 tctaattcaa caagaattgg gacaactcca gtgaaaagtt cttctccttt actcatctta
     8521 aacctcctta cctcgtaaac tattaaacaa aattatttgt agaggctgtt tcgtcctcac
     8581 ggactcatca gaccggaaag cacatccggt gacagctgtg cacgcccgga tgatcctgac
     8641 gacggagacc gccgtcgtcg acaagccggc cgttaattaa cctcccgtcc gcacagggct
     8701 cgctggtgac cggtccatgt tgtcggcata cgaggtattg aagattgggc taagtgcccc
     8761 aactggggta acctttgagt tctctcagtt ggggggctag caaagttaaa caaaattatt
     8821 tgtagaggga aaccgttgtg gtctccctga atatattata cgagccttat gcatgcccgt
     8881 aaagttatcc agcaaccact catagaccta gggcagcaga tagggacgac gtggtgttag
     8941 ctgtgctcga gctgcaggcg aacacagtgg tacgattcat gattctgcat tcgatccagg
     9001 ttaagagatg catctcgtat atatagtact tgtgcctgtg taacctgagc cttcagcatc
     9061 gttcctgcag ggacttgcga accccctgcc aaagtcgtaa tataggatcg gacaatcctg
     9121 tcttcgtggt aagggttgtt gcacgttggg gatagcgcta gctaacaaag acgcctgcta
     9181 caacaggagt cggaaacccg tacaaaggga acatccacac tacggtgaat cgaagcgcgg
     9241 catcaggatt tccttttgga tacctggggc aaagcccatc gtggtcgagg tgacttggca
     9301 cacttacacc tgcagcgcgc gcatgtggaa ttagaggcca agttcgatcc ctacaccgac
     9361 gtacgatgca actgtgtgga tgtgacgagc ttcatttata cgcttcgcgc gccggaccgg
     9421 cctccgcaag gcgcagcagt gcacaagcag tcatcaatta accaccgtgt attcggtatg
     9481 cagcatcagg cagtttgggc caaaacccca ggggccggtt tacacagaga cccgcatctt
     9541 gacctaactg acaaactgcc atggacgact agcccagcca acggctctta gacagcccga
     9601 tacagtgatt accccaggtt tgcggggcat ggctacgact tgctcagcta cgtgcgaggg
     9661 cagaaacttt tccgcatttg tatgttcacc tatttcagcc aaaaaactta agaccgccgg
     9721 tcttgtccac taccttgcag taatgcggtg gacaggatcg gcggttttct tttctcttct
     9781 caaatcgatg gagtacgcgc ccggggagcc caagggcacg ccctggcacc cgcagagctc
     9841 agctgtcacc ggatgtgctt tccggtctga tgagtccgtg aggacgaaac agcctctaca
     9901 aataattttg tttaaactaa caataaagta taaggaggtc taca
//
