LOCUS       sequence-178184-       12054 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Turning off/down miR390 in Soybean.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12054)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 12054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: TF101-STTM-miR390
FEATURES             Location/Qualifiers
     source          1..12054
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        66..411
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    572..748
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1310..1328)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1311..1328)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1967..1986
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2089..2094,2095..2111,2112..2117))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(2387..2403)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2387..2403)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2400..2422)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2411..2427
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2435..2441,2442..2459,2460..2465))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2480..2501
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    2659..2683
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4014..4643
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5072..6145
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6211..6405
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6641..6663)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6749..6889
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6804..6823
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6904..6921)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7075..7663)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7909..8700)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    9221..9245
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     9445..9467
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9459..9476
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9460..9476
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(10046..10594)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(10746..11090)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     11694..11713
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11694..11712
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gacgcacaat cccaccccta ctccaaaaat gtcaaagata ctgtctcaga agaccaaagg
       61 gctattgaga cttttcaaca aagggtaatt tcgggaaacc tcctcggatt ccattgccca
      121 gctatctgtc acctcatcga aaggacagta gaaaaggaag gtggctccta caaatgccat
      181 cattgcgata aaggaaaggc tatcattcaa gatgcctctg ccgacagtgg tcccaaagat
      241 ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag
      301 caagtggatt gatgtgacat ctccactgac ataagggatg acgcacaacc ccactatcct
      361 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac agcccaagct
      421 tggcgctatc ccctatcctg agcttgttgt tgttgttatg gtctaattta aatatggtct
      481 aaagaagaag aatggcgcta tcccctatcc tgagcttgaa ttcggtacgc tgaaatcacc
      541 agtctctctc tacaaatcta tctctctcta ttttctccat aaataatgtg tgagtagttt
      601 cccgataagg gaaattaggg ttcttatagg gtttcgctca tgtgttgagc atataagaaa
      661 cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa
      721 accaaaatcc agtactaaaa tccagatctc ctaaagtccc tatagatctt tgtcgtgaat
      781 ataaaccaga cacgagacga ctagacctgg agcccagacg ccgttcgaag ctagaagtac
      841 cgcttaggca ggaggccgtt agggaaaaga tgctaaggca gggttggtta cgttgactcc
      901 cccgtaggtt tggtttaaat atgatgaagt ggacggaagg aaggaggaag acaaggaagg
      961 ataaggttgc aggccctgtg caaggtaaga agatggaaat ttgatagagg tacgctacta
     1021 tacttatact atacgctaag ggaatgattg tatttatacc ctataccccc taataacccc
     1081 ttatcaattt aagaaataat ccgcataagc ccccgcttaa aaattggtat cagagccatg
     1141 aataggtcta tgaccaaaac tcaagaggat aaaacctcac caaaatacga aagagtcctt
     1201 aactctaaag ataaaagatc tttcaaaatc aaaactagtt ccctcacacc ggtgacgggg
     1261 atcgcatgcg acctctagac catcacattg gagcttcagc tgctcgagtt ctatagtgtc
     1321 acctaaatcg tatgtgtatg atacataagg ttatgtatta attgtagccg cgttcttacg
     1381 tcgacgcacc aattacgccc cgccctgtca ctcatcgcag tactgttgta attcattaag
     1441 cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat
     1501 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt
     1561 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac
     1621 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
     1681 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
     1741 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
     1801 tattaccagc tcaccgtctt tcattgccat acggaactcc ggatgagcat tcatcaggcg
     1861 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
     1921 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
     1981 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
     2041 ttctttctcc attttagctt ccttagctcc tgaaaatctc gataactcat taaagcttat
     2101 cgatgataag ctgtcaaaca tgagaattta gcccgcctaa tgagcgggct ttttgttgtg
     2161 atccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2221 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2281 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     2341 cctttcttaa ttaagacccg ggtaccgagc tcgaattcgt aatcatgtca tagctgtttc
     2401 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     2461 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     2521 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     2581 ggagaggcgg tttgcgtatt ggagcttgag cttggatcag attgtcgttt cccgccttca
     2641 gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt
     2701 ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt
     2761 atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttaaagt actttaaagt
     2821 actttaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc
     2881 agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc
     2941 cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac
     3001 ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg
     3061 ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc
     3121 ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga
     3181 gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga
     3241 ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg
     3301 cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat
     3361 ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac
     3421 ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct
     3481 caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa
     3541 agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag
     3601 cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac
     3661 cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg
     3721 caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc
     3781 gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc
     3841 ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa
     3901 accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg
     3961 gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg
     4021 ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag
     4081 cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca
     4141 gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc
     4201 gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg
     4261 gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga
     4321 ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta
     4381 agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga
     4441 tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca
     4501 ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa
     4561 ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg
     4621 aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa
     4681 cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct
     4741 ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa
     4801 gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat
     4861 cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta
     4921 aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt
     4981 gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg
     5041 gcactggaac ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt
     5101 acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc
     5161 ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga
     5221 tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc
     5281 gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg
     5341 cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc
     5401 tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc
     5461 cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga
     5521 atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca
     5581 cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct
     5641 ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc
     5701 caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat
     5761 cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta
     5821 ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt
     5881 gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc
     5941 agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa
     6001 gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt
     6061 gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga
     6121 gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc
     6181 aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc acgtacattg ggaacccaaa
     6241 gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg
     6301 gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc
     6361 tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg
     6421 cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc
     6481 cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg
     6541 caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca
     6601 aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     6661 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     6721 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
     6781 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     6841 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
     6901 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     6961 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     7021 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     7081 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7141 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7201 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     7261 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     7321 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     7381 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     7441 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     7501 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     7561 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     7621 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     7681 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgca
     7741 tgatatatct cccaatttgt gtagggctta ttatgcacgc ttaaaaataa taaaagcaga
     7801 cttgacctga tagtttggct gtgagcaatt atgtgcttag tgcatctaac gcttgagtta
     7861 agccgcgccg cgaagcggcg tcggcttgaa cgaatttcta gctagacatt atttgccgac
     7921 taccttggtg atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga
     7981 ggccaagcga tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg
     8041 atactgggcc ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt
     8101 gccggttact gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc
     8161 agcccagtcg ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc
     8221 ctgttcagga accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg
     8281 ttctcttgct tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat
     8341 acctgcaaga atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata
     8401 acgccacgga atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc
     8461 gctctctcca ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt
     8521 ttcatcaagc cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc
     8581 gccatccact gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc
     8641 gatgacgcca actacctctg atagttgagt cgatacttcg gcgatcaccg cttcccccat
     8701 gatgtttaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa
     8761 catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg
     8821 taccccaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct
     8881 gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagctt
     8941 acgaaccgaa caggcttatg tccactgggt tcgtgcccga attgatcaca ggcagcaacg
     9001 ctctgtcatc gttacaatca acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg
     9061 gcagcttagt tgccgttctt ccgaatagca tcggtaacat gagcaaagtc tgccgcctta
     9121 caacggctct cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt
     9181 ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata tattgtggtg
     9241 taaacaaatt gacgcttaga caacttaata acacattgcg gacgttttta atgtactgaa
     9301 ttaacgccga attgctctag cattcgccat tcaggctgcg caactgttgg gaagggcgat
     9361 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     9421 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca
     9481 agctaattcg cttcaagacg tgctcaaatc actatttcca cacccctata tttctattgc
     9541 actccctttt aactgttttt tattacaaaa atgccctgga aaatgcactc cctttttgtg
     9601 tttgtttttt tgtgaaacga tgttgtcagg taatttattt gtcagtctac tatggtggcc
     9661 cattatatta atagcaactg tcggtccaat agacgacgtc gattttctgc atttgtttaa
     9721 ccacgtggat tttatgacat tttatattag ttaatttgta aaacctaccc aattaaagac
     9781 ctcatatgtt ctaaagacta atacttaatg ataacaattt tcttttagtg aagaaaggga
     9841 taattagtaa atatggaaca agggcagaag atttattaaa gccgcgtaag agacaacaag
     9901 taggtacgtg gagtgtctta ggtgacttac ccacataaca taaagtgaca ttaacaaaca
     9961 tagctaatgc tcctatttga atagtgcata tcagcatacc ttattacata tagataggag
    10021 caaactctag ctagattgtt gagcagatct cggtgacggg caggaccgga cggggcggta
    10081 ccggcaggct gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc
    10141 cggccgcccg cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg
    10201 ggtcgttggg cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact
    10261 tcagcaggtg ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt
    10321 acacggtcga ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg
    10381 cgatgccggc gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac
    10441 ggacgaggtc gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc
    10501 ttgtctcgat gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac
    10561 ggcggatgtc ggccgggcgt cgttctgggc tcatggtaga tcccccgttc gtaaatggtg
    10621 aaaattttca gaaaattgct tttgctttaa aagaaatgat ttaaattgct gcaatagaag
    10681 tagaatgctt gattgcttga gattcgtttg ttttgtatat gttgtgttga gaattaattc
    10741 tcgaggtcct ctccaaatga aatgaacttc cttatataga ggaagggtct tgcgaaggat
    10801 agtgggattg tgcgtcatcc cttacgtcag tggagatatc acatcaatcc acttgctttg
    10861 aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt
    10921 tgggaccact gtcggcagag gcatcttcaa cgatggcctt tcctttatcg caatgatggc
    10981 atttgtagga gccaccttcc ttttccacta tcttcacaat aaagtgacag atagctgggc
    11041 aatggaatcc gaggaggttt ccggatatta ccctttgttg aaaagtctca attgcccttt
    11101 ggtcttctga gactgtatct ttgatatttt tggagtagac aagtgtgtcg tgctccacca
    11161 tgttatcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga
    11221 tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttcaacga
    11281 tggcctttcc tttatcgcaa tgatggcatt tgtaggagcc accttccttt tccactatct
    11341 tcacaataaa gtgacagata gctgggcaat ggaatccgag gaggtttccg gatattaccc
    11401 tttgttgaaa agtctcaatt gccctttggt cttctgagac tgtatctttg atatttttgg
    11461 agtagacaag tgtgtcgtgc tccaccatgt tgacctgcag gcatgcaagc ttgcatgcct
    11521 gcaggtcgac tctagagagt taattaagtt tgcaagcagc agattacgcg cagaaaaaaa
    11581 ggatctcaag gagatccttt ggatcacaaa aaaaaagccc gctcattagg cgggttaaat
    11641 tcccgggtac tgcagccgat tcattaatgc aggttaacct ggcttatcga aattaatacg
    11701 actcactata gggagaccgg cagatctgat ccctactcca aaaatgtcaa agatacagtc
    11761 tcagaagatc aaagggctat tgagactttt caacaaaggg taatttcggg aaacctcctc
    11821 ggattccatt gcccagctat ctgtcacttc atcgaaagga cagtggaaaa ggaaggtggc
    11881 tcctacaaat gccatcattg cgataaagga aaggctatca ttcaagatgc ctctgccgac
    11941 agtggtccca aagatggacc cccacccacg aggagcatcg tgggaaaaga agacgttcca
    12001 accacgtctt caaagcaagt ggattgatgt gacatctcca ctgacgtaag ggat
//
