LOCUS       sequence-178191-       11057 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  inducible lentiviral expression of PHF2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11057)
  AUTHORS   Pattabiraman DR, Bierie B, Kober KI, Thiru P, Krall JA, Zill C, 
            Reinhardt F, Tam WL, Weinberg RA
  TITLE     Activation of PKA leads to mesenchymal-to-epithelial transition and 
            loss of tumor-initiating ability.
  JOURNAL   Science. 2016 Mar 4;351(6277):aad3680. doi: 10.1126/science.aad3680.
  PUBMED    26941323
REFERENCE   2  (bases 1 to 11057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-03-4"; volume: "351(6277):aad3680. doi"; pages: " 
            10.1126/science.aad3680"
COMMENT     Sequence Label: pLIX402 PHF2
FEATURES             Location/Qualifiers
     source          1..11057
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        32..346
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    40..58
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    76..94
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    111..129
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    147..165
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    183..201
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    218..236
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    254..272
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     319..343
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    375..399
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3718..3742)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3750..3776
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3792..3809
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        3819..4329
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4248..4266
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4339..4935
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4339..4358)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4835..4855
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4936..4989
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4990..5607,5608..5613,5614..5649,5650..5652,5653..5688,
                     5689..5691,5692..5727,5728..5736)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5776..5796)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6194..6205)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6410..6643
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6715..6836
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6752..6771)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6806..6825
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6876..7011
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7011..7082
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7083..7151,7152..7943)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7301..7320)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8114..8702
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8603..8622
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8856..8873
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8990..9011
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9026..9031,9032..9049,9050..9056)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9064..9080
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9069..9091
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9088..9104
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9088..9104
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9123..9143
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9125..9143
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9171..9397
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9398..9578
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9625..9750
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10243..10476
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10661..10705
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10918..11035
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
       61 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      121 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      181 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      241 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      301 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      361 gcatcgattg atcaacaagt ttgtacaaaa aagcagaagg gccgtcaagg cccaccatgg
      421 cgacggtgcc cgtgtactgc gtctgccggc tcccctacga cgttacccgc ttcatgatcg
      481 agtgcgacgc ctgcaaggac tggttccacg gcagctgtgt tggggtggaa gaggaggagg
      541 cgcccgacat cgacatatac cactgcccaa actgtgagaa aacccatggg aagtccacct
      601 taaagaagaa gcggacctgg cacaaacacg gcccggggca agcgcctgac gtcaagcccg
      661 tgcagaatgg cagccagctc ttcatcaagg agctgcggag ccggaccttt cccagtgctg
      721 aagacgtggt ggcccgtgtg ccaggaagtc agctcacgct gggctacatg gaggagcacg
      781 gcttcaccga gcccatcctc gtccctaaga aagacgggct gggtctggct gtcccggccc
      841 ccacgttcta tgtcagtgac gtcgagaact acgtggggcc ggaacggagt gtggatgtga
      901 cagatgtcac caagcagaag gactgcaaga tgaagctgaa ggagtttgtg gactattact
      961 acagcaccaa ccgcaagcgg gtcctcaacg tcaccaacct cgagttctct gacacccgaa
     1021 tgtccagctt cgtggagcca cctgacattg taaagaaact gtcatgggta gaaaactact
     1081 ggccagatga tgcattgctg gccaagccca aagtgaccaa gtactgccta atctgcgtga
     1141 aggacagtta caccgacttc cacatcgact ctgggggcgc ctctgcctgg taccacgtgc
     1201 tcaaggggga gaagaccttc tatctcatca ggccggcctc ggccaacatc tccctgtatg
     1261 agcgctggcg gtctgcctct aaccacagcg agatgttctt tgctgaccag gtcgacaaat
     1321 gctacaagtg catcgtcaag cagggccaga ccctcttcat cccctcaggc tggatctacg
     1381 ccacactcac ccctgtggac tgcctggcct tcgcgggaca tttcctccac agcctgagtg
     1441 tggagatgca gatgagagca tatgaagtgg aaaggaggtt gaaactgggc agcctgactc
     1501 agtttcccaa ctttgaaact gcgtgctggt acatggggaa gcacctgctg gaggcattca
     1561 aaggctctca caaatctggg aagcagctgc cccctcatct agtccaagga gctaaaattc
     1621 tcaatggtgc tttccgatcg tggacgaaga agcaggcttt ggcagagcat gaggacgagc
     1681 tcccggagca cttcaaacct tcacagctaa tcaaagacct ggccaaagag atccggctca
     1741 gtgagaatgc ctccaaagcc gtccgaccgg aagtgaatac tgtcgcctcg tcagatgagg
     1801 tgtgtgacgg ggaccgggag aaggaggagc ccccgtctcc cattgaggcc accccgcctc
     1861 aatccctcct ggagaaagtg tccaaaaaaa agactcccaa aactgtgaag atgcccaagc
     1921 catccaaaat ccccaagccc ccgaagcccc ctaagccccc aaggcccccc aaaacgctga
     1981 agctcaaaga tggaggcaag aagaaaggga agaagtcccg ggagtcagcc tcacccacca
     2041 tccccaacct ggacctgctc gaagcccaca ccaaggaggc actgaccaag atggagccgc
     2101 ccaagaaggg caaggccaca aagagtgtcc tgagtgtgcc caacaaagat gtggttcaca
     2161 tgcagaatga tgtggagagg ctggaaattc gagagcaaac caagagcaag tcagaggcca
     2221 agtggaagta caagaacagc aaacctgact ccttactgaa gatggaagag gagcagaagc
     2281 tagagaagtc gcctctagct ggaaacaaag acaataagtt ctctttttct ttctccaaca
     2341 agaaactcct cggctccaag gctctcaggc ccccgacgag ccctggtgtg ttcggggcct
     2401 tgcagaactt caaggaggac aagcccaagc ccgtgcggga tgagtatgag tacgtgtcgg
     2461 atgacggtga gctcaagatc gacgagtttc ccatcaggag gaagaaaaac gccccgaaaa
     2521 gggacttgtc cttcttgttg gataagaagg ctgtgctgcc cacgcctgtc acgaagccaa
     2581 agctggactc ggcagcgtac aagagtgatg actcctcgga cgagggttcg ctgcacatcg
     2641 acacagacac caagcccggc cgcaatgcca gagtcaagaa ggagagtggg agctcggcag
     2701 ctggcatctt ggacctgctg caggccagtg aggaggttgg cgcgctggag tacaacccca
     2761 gcagccagcc cccggcctcc cccagcacac aggaagccat tcagggaatg ctgtccatgg
     2821 ccaacctgca ggcctccgac tcctgcctgc agaccacgtg gggagctggc caggccaagg
     2881 ggagctcgct ggctgcccat ggtgcccgga agaatggggg tggcagtggc aagagtgcag
     2941 gcaaacgact gctgaagagg gctgccaaga acagtgtcga cctggacgac tacgaggaag
     3001 agcaggacca cctggatgcc tgcttcaagg actcagacta cgtttacccc tcactggagt
     3061 cagatgaaga caaccccatc tttaagtccc ggtcgaagaa aaggaaaggc tcagacgacg
     3121 ctccctacag cccaacagca agggtcggcc catcggtgcc aagacaggac aggcctgtgc
     3181 gtgagggtac acgggtggct tccatcgaga ccgggctggc ggctgctgca gctaagttgt
     3241 cccagcagga ggagcagaaa agcaagaaaa aaaagagtgc caagaggaag ctgactccta
     3301 acaccacctc cccttccacc tccacctcca tctctgccgg caccacctcc acctccacca
     3361 cgccagcctc taccaccccg gcctccacca ccccggcctc caccagcacg gccagcagcc
     3421 aggcctcgca ggagggcagc tcgccagagc ccccgcctga gtcgcatagc agcagcctgg
     3481 cggaccatga gtacacagcc gctggcacct tcaccggggc ccaggctggc cgcacctccc
     3541 agcccatggc ccctggggtc tttctcacac agaggcggcc ctccgcatcg tctccaaaca
     3601 acaacaccgc tgccaaagga aaacgtacga aaaagggcat ggcgaccgcc aagcagaggc
     3661 ttgggaaaat tttgaaaatt catcggaacg ggaaactact cctttaaggc ctcatgggcc
     3721 cagctttctt gtacaaagtg gttaccggtt atccgtatga tgtgccggat tatgcgtaaa
     3781 cgcgtccggt ccaccaccac caccaccact aaggatccgg ggttggggtt gcgccttttc
     3841 caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc
     3901 agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc
     3961 ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa
     4021 ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt
     4081 accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg
     4141 gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg
     4201 gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc
     4261 tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc
     4321 tctccccagc aattcaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     4381 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     4441 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     4501 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     4561 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     4621 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     4681 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     4741 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     4801 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     4861 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     4921 cgcaagcccg gtgccgaagg tagaggttct ctcctcactt gtggtgatgt tgaagaaaac
     4981 cctggtccaa tgtctagact ggacaagagc aaagtcataa acggagctct ggaattactc
     5041 aatggtgtcg gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct gggagttgag
     5101 cagcctaccc tgtactggca cgtgaagaac aagcgggccc tgctcgatgc cctgccaatc
     5161 gagatgctgg acaggcatca tacccacttc tgccccctgg aaggcgagtc atggcaagac
     5221 tttctgcgga acaacgccaa gtcataccgc tgtgctctcc tctcacatcg cgacggggct
     5281 aaagtgcatc tcggcacccg cccaacagag aaacagtacg aaaccctgga aaatcagctc
     5341 gcgttcctgt gtcagcaagg cttctccctg gagaacgcac tgtacgctct gtccgccgtg
     5401 ggccacttta cactgggctg cgtattggag gaacaggagc atcaagtagc aaaagaggaa
     5461 agagagacac ctaccaccga ttctatgccc ccacttctga gacaagcaat tgagctgttc
     5521 gaccggcagg gagccgaacc tgccttcctt ttcggcctgg aactaatcat atgtggcctg
     5581 gagaaacagc taaagtgcga aagcggcggg ccgaccgacg cccttgacga ttttgactta
     5641 gacatgctcc cagccgatgc ccttgacgac tttgaccttg atatgctgcc tgctgacgct
     5701 cttgacgatt ttgaccttga catgctcccc gggtaactaa gtaaggatcg atccaagata
     5761 tcgtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     5821 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     5881 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     5941 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     6001 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     6061 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     6121 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     6181 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     6241 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     6301 cgcctccccg cctgtttcgc ctcggcgtcc ggactagagg tacctttaag accaatgact
     6361 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     6421 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     6481 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     6541 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     6601 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     6661 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     6721 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     6781 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     6841 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     6901 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     6961 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctttcaaata
     7021 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     7081 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     7141 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     7201 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     7261 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     7321 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     7381 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     7441 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     7501 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     7561 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     7621 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     7681 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     7741 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     7801 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     7861 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     7921 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     7981 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     8041 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     8101 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     8161 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     8221 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     8281 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     8341 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     8401 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     8461 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     8521 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     8581 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     8641 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     8701 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     8761 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     8821 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     8881 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     8941 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     9001 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     9061 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     9121 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
     9181 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
     9241 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
     9301 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
     9361 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
     9421 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     9481 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     9541 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     9601 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     9661 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     9721 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     9781 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     9841 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     9901 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     9961 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
    10021 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
    10081 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
    10141 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
    10201 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
    10261 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
    10321 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
    10381 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
    10441 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
    10501 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
    10561 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
    10621 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
    10681 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
    10741 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
    10801 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
    10861 cccaaccccg aggggacaat tctcgacctc gagacaaatg gcagtattca tccacaattt
    10921 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
    10981 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
    11041 ttattacagg gacagca
//
