LOCUS       sequence-17828-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Furman C, Short SM, Subramanian RR, Zetter BR, Roberts TM
  TITLE     DEF-1/ASAP1 is a GTPase-activating protein (GAP) for ARF1 that 
            enhances cell motility through a GAP-dependent mechanism.
  JOURNAL   J Biol Chem. 2002 Mar 8. 277(10):7962-9.
  PUBMED    11773070
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2002 Mar 8. 277(10):7962-9."
COMMENT     Sequence Label: pcDNA3 HA Arf1 ActQ71L
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             575..601
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(676..694)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(677..694)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(714..731)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    720..831
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 tagtacggcc gccagtgtgc tggaattcca tatggggaat atctttgcaa acctcttcaa
       61 gggccttttt ggcaaaaaag aaatgcgcat tctcatggtg ggcctagatg ctgcgggaaa
      121 gaccaccatc ctgtacaaac tgaagctggg tgaaattgtg accaccattc ccactattgg
      181 cttcaacgtg gagaccgtgg agtacaagaa catcagcttc actgtgtggg acgtgggtgg
      241 cctggacaag atccggcccc tgtggcgcca ctacttccag aacacacaag gcctgatctt
      301 cgtggtggac agcaatgaca gagagcgtgt gaacgaggcc cgtgaggagc tcatgaggat
      361 gctggccgag gacgagctcc gggatgctgt cctcctggtg ttcgccaaca agcaggacct
      421 ccccaacgcc atgaatgcgg ccgagatcac agacaagctg gggctgcact cactacgcca
      481 caggaactgg tacattcagg ccacctgcgc caccagcggc gacgggctct atgaaggact
      541 ggactggctg tccaatcagc tccggaacca gaagtaccca tacgatgttc cagattacgc
      601 ttaatagtct agacagctga gatctgatat ccatcacact ggcggccgct cgagcatgca
      661 tctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat cagcctcgac
      721 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
      781 gggaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
      841 gagtaggtgt cattctattc tggggggtgg gggtggggca ggacagcaag ggggaggatt
      901 ggga
//
