LOCUS       sequence-178308-        5075 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCMV-myc with cDNA encoding HsTRAPPC11 aa residues 701-1133.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Schou KB, Morthorst SK, Christensen ST, Pedersen LB
  TITLE     Identification of conserved, centrosome-targeting ASH domains in 
            TRAPPII complex subunits and TRAPPC8.
  JOURNAL   Cilia. 2014 Jun 18;3:6. doi: 10.1186/2046-2530-3-6. eCollection 
            2014.
  PUBMED    25018876
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cilia."; 
            date: "2014-06-18"; volume: "3:6. doi"; pages: " 
            10.1186/2046-2530-3-6. eCollection 2014"
COMMENT     Sequence Label: LP377
FEATURES             Location/Qualifiers
     source          1..5075
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          646..742
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    2188..2322
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2212..2231)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2266..2285
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2433..2449)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2433..2449)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2446..2468)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2457..2473
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2481..2487,2488..2505,2506..2511))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2526..2547
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2664..2681)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2835..3423)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2915..2934)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3594..4385,4386..4454))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4217..4236
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4455..4559)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4627..4645
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4683..4705)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4805..4824
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5018..5040
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5032..5049
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5033..5049
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
      601 agaggatccg gtactagagg aactgaaaaa ccagaaagtt aactggtaag tttagtcttt
      661 ttgtctttta tttcaggtcc cggatccggt ggtggtgcaa atcaaagaac tgctcctcag
      721 tggatgttgc ctttacttct aggcctgtac ggaagtgtta cttctgctct aaaagctgcg
      781 gaattgtacc cgcgggccca ccatggcatc aatgcagaag ctgatctcag aggaggacct
      841 gcttatggcc atggaggccc gaattctctt aaattggcag ggaggaggag gagatgctgc
      901 ttcctcccaa gaagccttac aggcagctcg gtctttcaaa aggcgaccta agctacctga
      961 caatgaagtt cactgggaca gcattataat tcaggcaagc acaatgatca tatccagagt
     1021 cccaaacatt tctgtacatc tgctacatga accccctgca ctgactaatg aaatgtattg
     1081 tttggttgtg actgttcagt cccatgaaaa gacccaaatc agagatgtga agctcaccgc
     1141 tggcttaaaa ccaggacagg atgccaattt aactcagaag actcacgtga ctcttcatgg
     1201 aacagaactg tgtgatgaat cctacccggc tttactcact gacattcctg ttggagactt
     1261 acatccaggg gaacagctgg aaaaaatgtt gtatgttcgc tgtggaacag tgggttccag
     1321 aatgtttctt gtatatgttt cttacctgat aaatacaacc gttgaagaaa aagaaattgt
     1381 ttgcaagtgt cacaaggatg aaactgtaac aattgaaaca gtctttccat ttgatgttgc
     1441 ggttaaattt gtttctacca agtttgagca cctggaaagg gtttatgctg acatcccctt
     1501 tctgttgatg acggacctct taagtgcctc accctgggcc ctcactattg tttccagtga
     1561 gctccagctt gctccatcca tgaccacagt ggaccagctc gagtctcaag tggacaatgt
     1621 tatcttacag actggagaga gtgctagtga atgcttttgt cttcaatgcc catctcttgg
     1681 aaatattgaa ggtggagtag caaccgggca ttatattatc tcttggaaaa ggacctcagc
     1741 aatggagaat atccccatca tcacaactgt catcactctg ccgcacgtga ttgtggagaa
     1801 tatccctctc catgtgaatg cagatctgcc gtcatttggg cgtgtcagag agtcgttacc
     1861 tgtcaagtat cacctacaga ataagaccga cttagttcaa gatgtagaaa tttctgtgga
     1921 gcccagtgat gccttcatgt tctcaggtct caaacagatt cgattacgta tcctccctgg
     1981 cacggagcag gaaatgctat ataatttcta tcctctgatg gctggatacc agcagctgcc
     2041 atctctcaac atcaacttgc ttagatttcc taacttcaca aatcagctgc tcaggcgttt
     2101 tatacctacc agtatttttg tcaagccaca gggtcgactc atggatgata cctctattgc
     2161 tgctgcatga ggtaccgcgg ccgcggggat ccagacatga taagatacat tgatgagttt
     2221 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     2281 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     2341 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttcggatcc tctagagtcg
     2401 atctgcaggc atgctagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     2461 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     2521 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     2581 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     2641 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     2701 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     2761 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     2821 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     2881 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     2941 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     3001 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     3061 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     3121 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     3181 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     3241 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     3301 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     3361 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     3421 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     3481 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     3541 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     3601 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     3661 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     3721 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     3781 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     3841 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     3901 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     3961 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     4021 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     4081 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     4141 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     4201 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     4261 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     4321 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     4381 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     4441 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     4501 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     4561 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     4621 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     4681 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     4741 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     4801 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     4861 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt
     4921 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     4981 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     5041 acggccagtg agttcgagct tgcatgcctg caggt
//
