LOCUS       sequence-178328-        4261 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses S. baltica TtrS constitutively.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Daeffler KN, Galley JD, Sheth RU, Ortiz-Velez LC, Bibb CO, Shroyer 
            NF, Britton RA, Tabor JJ
  TITLE     Engineering bacterial thiosulfate and tetrathionate sensors for 
            detecting gut inflammation.
  JOURNAL   Mol Syst Biol. 2017 Apr 3;13(4):923. doi: 10.15252/msb.20167416.
  PUBMED    28373240
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2017-04-3"; volume: "13(4):923. doi"; pages: " 
            10.15252/msb.20167416"
COMMENT     Sequence Label: pKD238-1a
FEATURES             Location/Qualifiers
     source          1..4261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     163..180
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        334..368
                     /label=BBa_J23109
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.04"
     regulatory      1496..1505
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      2437..2508
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2524..2551
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             2751..3542
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     terminator      3554..3648
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      join(3762..4261,1..46)
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     4208..4227
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg gctttgccgc
       61 ggccctctca cttccctgtt aagtatcttc ctggcatctt ccgggaaatc tccgccccgt
      121 tcgtaagcca tttccgctcg ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag
      181 gaagcggaat atatccccta ggcgttcggc tgcggcgagc ggtatcagct cactcaaagg
      241 cggtaatacg taaatcactg cataattcgt gtcgctcaag gcgcactccc gttctggata
      301 atgttttttg cgccgacatc ataacggttc tggtttacag ctagctcagt cctagggact
      361 gtgctagcat aatcattaac aacatcttat atcaaatttt aatatgtttt cttccggcat
      421 gagcctgaaa tggcctctga gcatccaggg ttccatcgaa ccgaaaatga ctccgcgcat
      481 catcttccgc atggccctgc gcactaaagt gttcatcacc ctgctggctt gcctgtttgc
      541 ttctgccaac gtattcgcgg tggaaccgca gaaccagtcc gcgccttccg atgtgatgga
      601 cgctgaggtg gttgctccgg ttgcgacgga ggaaccgggt taccaggtcg ttgatgttgg
      661 tgtgctggcg attcgcggtt ataaagctac catcaaccgt tggcagccac tgatgggttg
      721 gctggagact cagatcccga actcctactt ccgcctgcac ccgctgtctc tggacgagct
      781 ggcaaagggt gtagaaaccc agggtctgga cttcgtaatc accaatccag gccagtccgt
      841 actgctggcg cgtcagtatt ctctgacgtg gctggcgacc ctgcgctctc cgctgaacaa
      901 cggtgctgcg atgcaggtgg gttccgcgct ggtagttcgt gccgacagcc cgtaccagac
      961 cctgaccgat ctgaaaggcc gtcgtctggg catcgtcagc aaaaacgcat tcggtggtta
     1021 tctgacgctg gtatatgagg cacagctgaa aggcatcgat ctgccgcgct ttgttggtga
     1081 aatcatccca ctgggcttcc cgctggataa cctgctgtat cagctggatg atcagaaatc
     1141 tggtaatgac gccgcaaaag acaatcgtct ggatgccacc gttgtaccgg tatgccaact
     1201 ggaacagatg caggccgaag gtctgatcaa catcggccac tatcgcgttc tggacaacca
     1261 gaccccggta ggtttccact gtcaagtaag cactcgtctg tatccgaact ggtctatggc
     1321 taaaactaac cgtgccagcc agtccctggc aaaatctgtg acccaggctc tgctggctct
     1381 gccggaagat cacctggcag cgaaaagctc tgactctgca ggttggacca ctgcagtgtc
     1441 ccagctggcg atcgaccagc tgctgaaaga cctggacatg catccactgc agaccccatg
     1501 gtggcagcgc gcttggcagt gggtaaaact gcaccagcag tgggcgtggt tcattctggg
     1561 tatcctggtc ctgctgaacg cataccattt ctggctggaa taccgtttct cccgtcgtgg
     1621 tcgtgaactg atcaacaccc agcgccagct gaacgaaaac cgtgctctgc tggagcacgc
     1681 acaacgtatc gccatcgctg gcgaactggg tgctagcctg tcccatgaac tgaaccagcc
     1741 gctggctgca attggtcact attgccatgg tgcggaagtg cgtctgcagc gtggtacttc
     1801 tcctgaagaa ctgcagtctg ttctgaccct gatccagcag gaagttacgc gcgccgactc
     1861 tatcatcagc cgtctgcgta acctgctgaa aaaacgcccg gttagcaaac aggctctgta
     1921 tctgcacgaa ctggtaaacg aaacggtgcc gctgctggcg tacgaattcg aacagcacca
     1981 gatcaacctg gcggtaaacg tcagcggtga accttacctg caatctctgg acgaggtcgg
     2041 tatgcagcag ctgctgctga acctgctgaa aaacgcgttt gacgcgtgcg ttcagcgcct
     2101 ggaactggaa tcctccggca ctgaacagag cggtatgcgt aaaccgtatg ttcctacgat
     2161 cgatattgat ctgcgctacc aggagcgtac tctgctgctg actgtgacgg ataacggcac
     2221 tggtctgacc gaggagacta gcctgctgat gcaggccttc tattccacca aatctgaagg
     2281 cctgggcctg ggtctggtta tttgccgtga tatcgctgag tcccacggtg gcaccttctc
     2341 tctggaatcc gccatgggtg gtggctgcca ggctcaggtg gccatcccgc gcaagccgga
     2401 accgaatggc gttctgtaat aatctagacc aggcatcaaa taaaacgaaa ggctcagtcg
     2461 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     2521 actggctcac cttcgggtgg gcctttctgc gtttataggt acccggggat cctctagagt
     2581 cgacctgcag gcatgcaagc ttagcaagcg aaccggaatt gccagctggg gcgccctctg
     2641 gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca aggatctgat
     2701 ggcgcagggg atcaagatct gatcaagaga caggatgagg atcgtttcgt atgagggaag
     2761 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     2821 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     2881 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     2941 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     3001 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     3061 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     3121 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     3181 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     3241 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     3301 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     3361 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     3421 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     3481 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     3541 aagagctcgc ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat
     3601 ttgttcagaa cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aagcactagt
     3661 aacaacttat atcgtatggg gctgacttca ggtgctacat ttgaagagat aaattgcact
     3721 gaaatctagt aatattttat ctgattaata agatgatctt cttgagatcg ttttggtctg
     3781 cgcgtaatct cttgctctga aaacgaaaaa accgccttgc agggcggttt ttcgaaggtt
     3841 ctctgagcta ccaactcttt gaaccgaggt aactggcttg gaggagcgca gtcaccaaaa
     3901 cttgtccttt cagtttagcc ttaaccggcg catgacttca agactaactc ctctaaatca
     3961 attaccagtg gctgctgcca gtggtgcttt tgcatgtctt tccgggttgg actcaagacg
     4021 atagttaccg gataaggcgc agcggtcgga ctgaacgggg ggttcgtgca tacagtccag
     4081 cttggagcga actgcctacc cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc
     4141 ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc acgagggagc
     4201 cgccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac cactgatttg
     4261 a
//
