LOCUS       sequence-178340-        5708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent fragment of gamma-tubulin that lacks gamma-tubulin DNA 
            binding domain and NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Hoog G, Zarrizi R, von Stedingk K, Jonsson K, Alvarado-Kristensson M
  TITLE     Nuclear localization of gamma-tubulin affects E2F transcriptional 
            activity and S-phase progression.
  JOURNAL   FASEB J. 2011 Nov;25(11):3815-27. doi: 10.1096/fj.11-187484. Epub 
            2011 Jul 25.
  PUBMED    21788450
REFERENCE   2  (bases 1 to 5708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1096/fj.11-187484"; journalName: "FASEB J"; date: "2011-11"; 
            volume: "25"; issue: "11"; pages: "3815-27"
COMMENT     Sequence Label: pEGFPN1-N-gamma-tubulin (1-333)
FEATURES             Location/Qualifiers
     source          1..5708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        140..443
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        444..647
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     598..618
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1727..1736
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1733..1735,1736..1738,1739..2452)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1733..1735,1736..1738,1739..2449)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1778..1799)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2039..2058)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2386..2407
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2575..2696
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2612..2631)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2666..2685
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2703..3158)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2840..2861)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3052..3071
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3185..3289
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3291..3648
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3291..3482
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3314..3334)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3499..3634
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3683..4477
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3683..4477
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3737..3756)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4347..4366
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4665..4684)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4709..4756
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5085..5673
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5574..5593
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
       61 ccgtattacc gccatgcatt agttattaat agtaatcaat tacggggtca ttagttcata
      121 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      181 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      241 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      301 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      361 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      421 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
      481 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      541 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
      601 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc
      661 gtcagatccg ctagcgatgc cgagggaaat catcacccta cagttgggcc agtgcggcaa
      721 tcagattggg ttcgagttct ggaaacagct gtgcgccgag catggtatca gccccgaggg
      781 catcgtggag gagttcgcca ccgagggcac tgaccacaag gacgtctttt tctaccaggc
      841 agacgatgag cactacatcc cccgggccgt gctgctggac ttggaacccc gggtgatcca
      901 ctccatcctc aactccccct atgccaagct ctacaaccca gagaacatct acctgtcgga
      961 acatggagga ggagctggca acaactgggc cagcggattc tcccagggag aaaagatcca
     1021 tgaggacatt tttgacatca tagaccggga ggcagatggt agtgacagtc tagagggctt
     1081 tgtgctgtgt cactccattg ctggggggac aggctctgga ctgggttcct acctcttaga
     1141 acggctgaat gacaggtatc ctaagaagct ggtgcagaca tactcagtgt ttcccaacca
     1201 ggacgagatg agcgatgtgg tggtccagcc ttacaattca ctcctcacac tcaagaggct
     1261 gacgcagaat gcagactgtg tggtggtgct ggacaacaca gccctgaacc ggattgccac
     1321 agaccgcctg cacatccaga acccatcctt ctcccagatc aaccagctgg tgtctaccat
     1381 catgtcagcc agcaccacca ccctgcgcta ccctggctac atgaacaatg acctcatcgg
     1441 cctcatcgcc tcgctcattc ccaccccacg gctccacttc ctcatgaccg gctacacccc
     1501 tctcactacg gaccagtcag tggccagcgt gaggaagacc acggtcctgg atgtcatgag
     1561 gcggctgctg cagcccaaga acgtgatggt gtccacaggc cgagaccgcc agaccaacca
     1621 ctgctacatc gccatcctca acatcatcca gggagaggtg gaccccaccc aggtcaagct
     1681 tcgaattctg cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca ccatggtgag
     1741 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     1801 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     1861 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     1921 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     1981 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     2041 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     2101 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     2161 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     2221 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     2281 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     2341 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     2401 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg
     2461 cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     2521 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     2581 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     2641 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc
     2701 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     2761 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     2821 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     2881 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     2941 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     3001 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     3061 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     3121 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa
     3181 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3241 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga
     3301 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     3361 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     3421 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     3481 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     3541 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     3601 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga
     3661 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     3721 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     3781 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     3841 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac
     3901 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     3961 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     4021 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     4081 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     4141 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     4201 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     4261 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     4321 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     4381 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     4441 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     4501 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     4561 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     4621 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag
     4681 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt
     4741 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
     4801 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
     4861 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
     4921 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     4981 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     5041 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5101 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     5161 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5221 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5281 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5341 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     5401 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     5461 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     5521 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     5581 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     5641 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     5701 ttcctggc
//
