LOCUS       sequence-178448-       10028 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pEGFP-N1 with cDNA encoding full length HsKIF13A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10028)
  AUTHORS   Schou KB, Mogensen JB, Morthorst SK, Nielsen BS, Aleliunaite A, 
            Serra-Marques A, Furstenberg N, Saunier S, Bizet AA, Veland IR, 
            Akhmanova A, Christensen ST, Pedersen LB
  TITLE     KIF13B establishes a CAV1-enriched microdomain at the ciliary 
            transition zone to promote Sonic hedgehog signalling.
  JOURNAL   Nat Commun. 2017 Jan 30;8:14177. doi: 10.1038/ncomms14177.
  PUBMED    28134340
REFERENCE   2  (bases 1 to 10028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-01-30"; volume: "8:14177. doi"; pages: " 
            10.1038/ncomms14177"
COMMENT     Sequence Label: LP110a
FEATURES             Location/Qualifiers
     source          1..10028
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      5908..5917
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5914..5916,5917..5919,5920..6633)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5914..5916,5917..5919,5920..6630)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5959..5980)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6220..6239)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6567..6588
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6756..6877
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6793..6812)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6847..6866
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6884..7339)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7021..7042)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7233..7252
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7366..7470
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7472..7829
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7472..7663
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7495..7515)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7680..7815
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7864..8658
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7864..8658
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7918..7937)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8528..8547
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8846..8865)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8890..8937
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9266..9854
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9755..9774
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctcgagct caagcttatg tcggatacca aggtaaaagt tgccgtccgg
      601 gtccggccca tgaaccgacg agaactggaa ctgaacacca agtgcgtggt ggagatggaa
      661 gggaatcaaa cggtcctgca ccctcctcct tctaacacca aacagggaga aaggaaacct
      721 cccaaggtat ttgcctttga ttattgcttt tggtccatgg atgaatctaa cactacaaaa
      781 tacgctggtc aagaagtggt tttcaagtgc cttggggaag gaattcttga aaaagccttt
      841 caggggtata atgcgtgtat ttttgcatat ggacagacag gttcgggaaa atccttttcc
      901 atgatgggcc atgctgagca gctgggcctt attccaaggc tctgctgtgc tttatttaaa
      961 aggatctctt tggagcaaaa tgagtcacag acctttaaag ttgaagtgtc ctatatggaa
     1021 atttataatg agaaagttcg ggatctttta gaccccaaag ggagtagaca gtctcttaaa
     1081 gttcgagaac ataaagtttt gggaccatat gtagatggtt tatctcaact agctgtcact
     1141 agttttgagg atattgaatc attgatgtct gagggaaata agtctcgaac ggtagctgct
     1201 accaacatga acgaagaaag cagccgctcc catgctgtgt tcaacatcat aatcacacag
     1261 acactttatg acctgcagtc tgggaattcc ggggagaaag tcagtaaggt cagcttggta
     1321 gacctggcgg gtagcgaaag agtatctaaa acaggagctg caggagagcg actgaaagaa
     1381 ggcagcaaca ttaacaaatc gcttacaacc ttggggttgg ttatatcatc actggctgac
     1441 caggcagctg gcaagggtaa aagcaaattt gtgccttatc gagattcagt cctcacttgg
     1501 ctgcttaagg acaacttggg gggcaacagc caaacctcta tgatagccac aatcagccca
     1561 gccgcagaca actatgaaga gaccctctcc acattaagat atgcagaccg agccaaaagg
     1621 attgtgaacc atgctgttgt gaatgaggac cccaacgcaa aagtgatccg agaactgcgg
     1681 gaggaagtcg agaaactgag agagcagctc tctcaggcag aggccatgaa ggcccctgaa
     1741 ctgaaggaga agctcgaaga gtctgaaaag ctgataaaag aactaacagt gacttgggaa
     1801 gagaagctga ggaaaacaga agagatcgca caggaaagac aacgacaact tgaaagcatg
     1861 gggatttccc tggagatgtc cggtatcaag gtgggggatg acaaatgcta cttagtcaat
     1921 ctgaatgcag accctgctct taacgaactt ctggtttatt atttaaagga tcacaccagg
     1981 gtgggtgcag atacctctca agatatccag ctttttggca taggaattca gcctcagcac
     2041 tgtgagattg acattgcatc tgatggagac gtcactctca ctccaaaaga aaatgcaagg
     2101 tcctgtgtga acggcaccct tgtgtgcagt accacccagc tgtggcatgg tgaccgaatc
     2161 ctatggggaa ataatcactt ttttagaata aacttaccta agaggaaacg tcgagattgg
     2221 ttgaaagact ttgaaaaaga aacgggcccg ccagagcatg acctggatgc agccagtgag
     2281 gcttcctctg aaccagacta taactatgaa tttgcacaga tggaagttat catgaaaacc
     2341 ctgaatagta atgacccagt tcaaaatgtg gttcaggtcc tggagaaaca atacctagaa
     2401 gaaaagagaa gtgccctaga ggagcagcgg ctcatgtatg agcgggaact ggagcaactc
     2461 cgccagcagc tctcccccga caggcagcca cagagtagcg gccctgaccg cctggcctac
     2521 agcagccaga cagcgcagca gaaggtgacc cagtgggcag aagagaggga tgaactcttc
     2581 cgacaaagcc tggcaaaact gcgagagcag ctggttaaag ctaatacctt ggtgagggaa
     2641 gcaaacttcc tggctgagga aatgagcaaa ctcaccgatt accaagtgac tcttcagatc
     2701 cctgctgcaa acctcagtgc caataggaag agaggtgcaa tagtgagtga accagctatc
     2761 caagtgagga ggaaaggaaa gagcacccaa gtgtggacca ttgagaagct ggagaataaa
     2821 ttaattgaca tgagagacct ttaccaagaa tggaaggaaa aagttcctga ggcaaagaga
     2881 ctctacggaa aacgaggtga ccctttctat gaagcccaag aaaatcacaa cctcatcggg
     2941 gtggcgaatg tattcttgga atgcctcttc tgtgatgtga aacttcagta tgcagtccct
     3001 atcatcagcc agcaggggga ggttgcaggg cgtctccacg tggaagtgat gcgtgttaca
     3061 ggagctgttc cagagcgtgt ggtggaggat gactcttcgg agaattccag tgaaagtggg
     3121 agccttgaag tcgtagacag cagcggggaa atcattcacc gagtcaaaaa gctgacatgt
     3181 cgggtaaaaa ttaaagaagc aacggggctg cccttaaacc tctcaaattt tgtcttctgt
     3241 caatacacat tctgggacca gtgtgagtct acggtggctg ccccggtggt ggaccccgag
     3301 gtgccttcac cacagtccaa ggatgcccag tacacagtga ccttctccca ctgtaaggac
     3361 tatgtggtga atgtaacaga agaatttctg gagttcattt cagatggagc actggccatt
     3421 gaagtatggg gccaccggtg tgctggaaat ggcagctcca tctgggaggt cgattctctt
     3481 catgctaaga caagaacact gcatgacagg tggaatgaag taacgcgaag aatagaaatg
     3541 tggatctcca tattagaatt gaatgagtta ggagagtatg ctgcagtgga acttcatcag
     3601 gcaaaagatg tcaacacagg aggcatcttt caacttagac agggtcattc ccgtagagta
     3661 caagtcacgg tgaaacctgt gcagcattca gggacactgc cacttatggt tgaagccatc
     3721 ctgtcagtat ccatcggctg tgtaactgcc aggtccacca aactccaaag agggctggac
     3781 agttaccaga gagatgatga ggatggtgat gatatggata gttatcagga agaagactta
     3841 aactgcgtaa gggagaggtg gtcagatgca ctcattaaac gacgagaata cctggatgaa
     3901 cagataaaaa aagtcagcaa taaaacagag aaaacagagg acgatgtgga gcgggaagcc
     3961 cagcttgtgg agcagtgggt agggctgact gaggaaagga atgctgtgct ggtgccagcc
     4021 ccaggcagtg ggattcctgg ggcacctgcc gactggatcc cacctcctgg aatggaaacc
     4081 cacataccag ttctcttcct cgatttgaat gcggatgacc tcagtgccaa tgagcagctt
     4141 gttggccccc atgcatccgg cgtgaactcc atcctgccca aggagcatgg cagtcagttt
     4201 ttctacctgc ccatcataaa gcacagtgat gatgaggttt cagccacagc ctcttgggat
     4261 tcctcggtgc atgattctgt tcacttgaat agggtcacac cacagaatga aaggatttac
     4321 ctaattgtga aaaccacagt tcaactcagc caccctgctg ctatggagtt agtattacga
     4381 aaacgaattg cagccaatat ttacaacaaa cagagtttca cgcagagttt gaagaggaga
     4441 atatccctga aaaatatatt ttattcctgt ggtgtaacct atgaaatagt atccaatata
     4501 ccaaaggcaa ctgaggagat agaggaccgg gaaacgctgg ctctcctggc agcaaggagt
     4561 gaaaacgaag gcacatcaga tggggagacg tacattgaga agtacactcg aggcgtgctg
     4621 caggtggaaa acattctgag tcttgaacgg ctccggcagg ccgtcacagt caaagaagca
     4681 ctttccacca aagcccggca cattcggagg agcctcagta caccaaatgt tcataatgtc
     4741 tcttccagcc gaccggacct ttctggcttt gatgaagatg acaagggttg gccagagaac
     4801 cagttggaca tgtctgacta tagctccagt taccaagatg tagcatgtta tggaacttta
     4861 cccagggatt ctcctcgaag gaataaagaa ggttgtacat cagagactcc tcatgcctta
     4921 accgtcagcc cttttaaagc attctctcct cagccaccaa agtttttcaa gcccctaatg
     4981 cctgtaaaag aggagcataa gaaaaggata gccctggaag caaggcctct tctaagccag
     5041 gaggactctg aggaggaaga aaatgagctg gaagctatta acaggaagct aataagttca
     5101 cagccttatg tacctgtgga gtttgctgac ttcagtgttt acaatgccag cttggagaac
     5161 agggaatggt tttcctctaa agtagatctg tcaaactcac gggtcttgga gaaagaagtg
     5221 tcccgtagcc ctaccaccag cagtattacc agtggctact tttcccacag tgcctccaat
     5281 gccaccctgt ctgacatggt ggtcccttct agtgacagct cagaccagct ggccattcag
     5341 acgaaggatg cagactccac cgagcactcc acaccatcgc ttgtgcatga tttcaggccg
     5401 tcctcaaaca aagagttgac agaagtcgaa aaaggcttgg taaaggacaa gataattgtg
     5461 gtgccgctca aggaaaacag tgccttagcc aaagggagcc catcatccca gagcatccct
     5521 gagaaaaact ccaaatcact gtgcaggact ggctcatgtt cagaactaga tgcctgcccc
     5581 agcaaaatta gccagccagc caggggattc tgccccaggg aggtgacggt agaacacacc
     5641 accaacatcc ttgaagacca ttctttcaca gaatttatgg gagtgtcaga gggaaaagat
     5701 tttgatggtt tgacagattc ttctgctgga gagctttcca gtaggaggag tctaccaaat
     5761 aaaacaggcg gcaagactgt ctccgatggg ctccaccacc ccagccagct gcattccaag
     5821 ttagagaatg accaggtaat aattccagag gcagcctttt gggttctgtg ctgtcaacag
     5881 gtaccgcggg cccgggatcc accggtcgcc accatggtga gcaagggcga ggagctgttc
     5941 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     6001 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     6061 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     6121 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     6181 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     6241 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     6301 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     6361 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     6421 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     6481 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     6541 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     6601 atcactctcg gcatggacga gctgtacaag taaagcggcc gcgactctag atcataatca
     6661 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     6721 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     6781 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     6841 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat
     6901 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     6961 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     7021 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     7081 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     7141 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     7201 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     7261 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     7321 gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     7381 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     7441 atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg
     7501 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     7561 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7621 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     7681 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7741 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     7801 gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt
     7861 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     7921 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     7981 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     8041 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     8101 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     8161 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     8221 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     8281 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     8341 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc
     8401 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     8461 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     8521 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     8581 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     8641 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     8701 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     8761 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     8821 tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc
     8881 gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata
     8941 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg
     9001 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc
     9061 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata
     9121 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     9181 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     9241 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     9301 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     9361 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     9421 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     9481 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9541 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9601 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9661 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9721 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9781 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     9841 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     9901 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     9961 ccatgcatta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
    10021 gagttccg
//
