LOCUS       sequence-178510-        2958 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5' Gateway entry clone containing endothelial specific enhancer 
            sequence of cldn5b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Quillien A, Abdalla M, Yu J, Ou J, Zhu LJ, Lawson ND
  TITLE     Robust Identification of Developmentally Active Endothelial 
            Enhancers in Zebrafish Using FANS-Assisted ATAC-Seq.
  JOURNAL   Cell Rep. 2017 Jul 18;20(3):709-720. doi: 
            10.1016/j.celrep.2017.06.070.
  PUBMED    28723572
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.06"; journalName: "Cell Rep"; date: 
            "2017-07-18- 18"; volume: "20"; issue: "3"; pages: "709-720"
COMMENT     Sequence Label: p5Ecldn5bE1
FEATURES             Location/Qualifiers
     source          1..2958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      10..598
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     499..518
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     752..769
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(928..955)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1047..1133)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1182..1204
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1196..1213
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1197..1213
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1253..1348
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    1664..1787
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1859..1878)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1860..1878)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1883..1899)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1883..1899)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2001..2020)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2012..2821
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2079..2098)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
       61 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
      121 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
      181 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      241 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
      301 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      361 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      421 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      481 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      541 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      601 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      661 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
      721 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      781 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      841 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      901 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      961 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
     1021 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
     1081 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
     1141 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
     1201 aacgacggcc agtcttaagc tcgggcccga gttaacgcta ccatggagct ccaaataatg
     1261 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
     1321 ataatgccaa ctttgtatag aaaagttgca ggatttcaca ctatacaact ccatctgtcc
     1381 acagcccacg taactccgac cggctcaaat ctacatccac aggtcgagat cctccacgcc
     1441 gtgccagagg aggaggtctg gatgctttgt tttctgtgtt cacgtcctgc ccaatgtcag
     1501 cgatgaggtc ggatagatac gtgttgaatc ccagacacct ggcgtgcagt gagctcacac
     1561 cagaggaaac atctgcagag aacatgcaga tcagagcttc tgtgcacttc tgccgtctgt
     1621 ctggagagca ctcaggattc ctgcttatgt tatgtactgt atgcaagttt gtacaaaaaa
     1681 gttgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa
     1741 aaacagacta cataatactg taaaacacaa catatgcagt cactatgaac caactactta
     1801 gatggtatta gtgacctgta gaattcgagc tctagagctg cagggcggcc gcgatatccc
     1861 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     1921 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
     1981 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag
     2041 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
     2101 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt
     2161 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     2221 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
     2281 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga
     2341 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
     2401 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
     2461 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     2521 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
     2581 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
     2641 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
     2701 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     2761 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     2821 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg
     2881 cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     2941 ccccgtagaa aagatcaa
//
