LOCUS       sequence-178565-        9327 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ish1 mCherry insertion cassette with ura4+ gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9327)
  AUTHORS   Gu M, LaJoie D, Chen OS, von Appen A, Ladinsky MS, Redd MJ, Nikolova
            L, Bjorkman PJ, Sundquist WI, Ullman KS, Frost A
  TITLE     LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in 
            fission yeast and human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2166-E2175. doi: 
            10.1073/pnas.1613916114. Epub 2017 Feb 27.
  PUBMED    28242692
REFERENCE   2  (bases 1 to 9327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1613916114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-03-14- 14"; volume: "114"; issue: "11"; pages: 
            "E2166-E2175"
COMMENT     Sequence Label: pMGF172
FEATURES             Location/Qualifiers
     source          1..9327
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    5..21
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10..32
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             44..697
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     655..677
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             704..727
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             1322..2032
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1467..1485)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1735..1754
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      2053..2240
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2108..2132)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(2722..3516)
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     rep_origin      4125..4908
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(5864..5886)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6031..6049)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6117..6221
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6222..6290,6291..7082)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6440..6459)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7253..7841
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7742..7761
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7995..8012
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        8085..8162
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             8163..9245
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(8182..8201)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     protein_bind    9258..9279
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9294..9299,9300..9317,9318..9324)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ggaattgtga gcggataaca atttcacaca ggaaacagta ttcatgtccc ctatactagg
       61 ttattggaaa attaagggcc ttgtgcaacc cactcgactt cttttggaat atcttgaaga
      121 aaaatatgaa gagcatttgt atgagcgcga tgaaggtgat aaatggcgaa acaaaaagtt
      181 tgaattgggt ttggagtttc ccaatcttcc ttattatatt gatggtgatg ttaaattaac
      241 acagtctatg gccatcatac gttatatagc tgacaagcac aacatgttgg gtggttgtcc
      301 aaaagagcgt gcagagattt caatgcttga aggagcggtt ttggatatta gatacggtgt
      361 ttcgagaatt gcatatagta aagactttga aactctcaaa gttgattttc ttagcaagct
      421 acctgaaatg ctgaaaatgt tcgaagatcg tttatgtcat aaaacatatt taaatggtga
      481 tcatgtaacc catcctgact tcatgttgta tgacgctctt gatgttgttt tatacatgga
      541 cccaatgtgc ctggatgcgt tcccaaaatt agtttgtttt aaaaaacgta ttgaagctat
      601 cccacaaatt gataagtact tgaaatccag caagtatata gcatggcctt tgcagggctg
      661 gcaagccacg tttggtggtg gcgaccatcc tccaaaatcg gatctggaag ttctgttcca
      721 ggggcccctg gctgttccta attgggctgc ttattttgat actttgagct ccaaagagct
      781 ttctgcctgg attaaggaat acaaaaaaca ttaccatgga aaacttcacc catccaaaga
      841 tcgtgaacat cttttccaaa atgcctgtaa tatttatcgc gctattgctg aaaaggctga
      901 caaatctgtt cttcaatcga ttcaatcaaa gtttcctaag gcaactgttc ccggttatga
      961 ctcttggtct aatgaagatc taaagtctgc actcaaggaa tatggtgaca gtattggtaa
     1021 ggtgtttaat cgtaaagatg ctattgaacg cttaaagcgt catgatattt tattctatgg
     1081 tcctgttgtt gccgacaagg ctaaatcagg agtctctggc ttccttcgac gtgttgcttc
     1141 ttttatggga tttggcactc gcgacgtagc tgaaattaag ctaggagaaa acattcaaaa
     1201 ggtaaaagat actgcctcta aagcctcatc tgctgtatct tcagcaggcg actatgtcga
     1261 aactaatgta aagaaagccc aaagggtttt gggaggaacg ggcggtagtg gaggcactgg
     1321 tatggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     1381 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     1441 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     1501 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     1561 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     1621 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     1681 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     1741 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     1801 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     1861 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
     1921 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     1981 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt gaggcgcgcc
     2041 acttctaaat aagcgaattt cttatgattt atgattttta ttattaaata agttataaaa
     2101 aaaataagtg tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa attcttattc
     2161 ttgagtaact ctttcctgta ggtcaggttg ctttctcagg tatagtatga ggtcgctctt
     2221 attgaccaca cctctaccgg cagatccgct agggataaca gggtaatata gatctgttta
     2281 gcttgcctcg tccccgccgg gtcacccggc cagcgacatg gaggcccaga atacaacacc
     2341 aatgtttata accaagtttt atcttgtttg tctacatggt attttacatt catctacata
     2401 catctttcat tggctttgta catagttatc attacaagtc taaaaaaatt cactcttttc
     2461 ttattcaatg tcaatccaag agaaaagatt gtggtaatgt tgtaggagca tgtttaataa
     2521 attactatag caaattactt tttattccca aggtgtttat ctataatagt taatatttta
     2581 gtcgctacat aaaattttac caaagagtac ttgtatacta attctaaatg ccttctgaca
     2641 taaaacgcct aggaaaacaa acgcaaacaa ggcatcgact ttttcaataa ccaaccaaaa
     2701 aaattttaca ttagtctttt tttaatgctg agaaagtctt tgctgatatg ccttccaacc
     2761 agcttctcta tatctcttgg cttcgacaac aggattacga ccagctccat agactccacg
     2821 accaacaatg atgatgtcgc taccgcagtt tacaatcact tcttcaggag tacgatattg
     2881 ctgtcccagc ccgtctcctt taacatccaa gccgatacca ggggacatag ttatgtagtc
     2941 gctttgaagg ttaggaaatc gacgaccagc tataaagcca aagcaaaaat cggtatgctt
     3001 ctcaaaccat tctaaggttt tctctgtgta ggaaccagta gccaaagagc ctttggaaga
     3061 catttcagcc aaaagcaaga gaccacgtcc caaaggtaaa ccaacttctt tgaggccttg
     3121 tataataccc tcgcctggca ctgtatggca atttgtgata tgagcccaag aagcaatttt
     3181 gtacacacca gatgcatatt gtagcttgac ggtatttcca atgtctgcga atttgcgatc
     3241 ctcaaagata agaaaacgat gctttttacc taaggccacc agtttttcta ccatatcctg
     3301 gtcgaaatcc tcgacaacgt caatatgtgt cttgataaca cagacatagg gtccaatttt
     3361 atctaccaat tctaagattt cggatttctt cgtcaaatcg accgcgactg acaagttgct
     3421 ttgcttttct tccatcaaag ccaacaattc cttggcaatg ggatttttca tcccctcagc
     3481 tctagctgaa tagctttgaa atactctagc atccataact ttgcttttaa acctttaatt
     3541 tcgatccaag caaaaaagag gttcttggta ggacaatacg gtaagaaaac acgacatgtg
     3601 cagagatgcc gacgaagcat agttaaactg ggatggtaaa atcaattaag aatttataaa
     3661 gacaaaattg tataagtctc taaaacatct taattatacc tcacagaact atctaaaata
     3721 tattcacaaa gtgcaaacat tatcatgaaa aagaaccatt ttaatttaaa gcaagggcat
     3781 taaggcttat ttacagaatt tcttactttt gtaaagatta taaggctgat tatctttttc
     3841 accatgccaa aaattacaca agatagaatg gatgtttgaa attaaacgtg agtatacaaa
     3901 caaatacact aggtaaatcg aaacattttt ttctccatta agtaacaaat tcctatttag
     3961 agaaagaatg ctgagtagat taaataatct atacaaactt ttttaacaca aatgcataca
     4021 tatagccagt gggatttgta gctaagcttc cgaattcgag tctaactcct taaccactcg
     4081 gacatagtga cttatctgac actcattgta aattaatata tatataggca ttttgtttag
     4141 ttaaaggtac ttaagtaatt agtataaacg aaccaatttt ataatcagga agttaagtga
     4201 atggtagcac atgtcgtaaa aattgtgaat ttttattgaa taatatttta aatacaagcc
     4261 tttctagact aggtatactc ataaacatat atgagcaaaa ggatagagga gattacattg
     4321 catcttctac aaatttattt attgcccttt actgaaaaat taaataatga gtactaaatg
     4381 ataaaaagcg ctcagtacaa gaaagattgc aaaaatattg cattcttcat gaattaaagt
     4441 tgcatataag gcatattgaa agtaatagta ctaaaacagc agttagcgaa aattaataga
     4501 attatattcg caagacaatt gtgacattaa attaaaaaat tgtagaattt ttactatcct
     4561 ctttaacgcc atgagccttt ataaaaaggt taaattagtt ttaacattct ttttttgagt
     4621 aagagtttac aatttatcaa aacctgtgtt attatattca ttagtttcaa tttattagca
     4681 tctagagaaa aatcaattgg cagttacatt gttggaattt atgaagaaaa agaatctaca
     4741 acggagaata agttgctgat cgctttccct aaaattgtat attttgctga gcttattttg
     4801 acatttcgtt gaagttttct ctaattcgca ttcattttaa gtaaaacaat gagaaataaa
     4861 attacaaaaa atacaaatta aaatacaatt tttagctata attatagacg atgcccttgt
     4921 atcccattct gtctcgcttg cccctacttt ttatctttta tataccataa tgaacgctgc
     4981 cgctactaac cataccccga ttttacattt cggactccca aggacgtaca aaatagaaaa
     5041 ctatagaaaa aaataatcag aaaatagcat gtcatctctt tgtaaaacgc gtttgcaaga
     5101 agaaaggaaa caatggagaa gagatcatcc atttgtatgt aaattttagt aaacttgaag
     5161 aaatcactaa caacttctct tacttaggga ttctatgcaa aaccttgtaa atcatctgat
     5221 ggaggactcg atttaatgaa ttggaaggtt gatactaacg ccgccatcca taaattgttc
     5281 atgaacgttc ttaatgtcta tttatattat ttaaaaagaa ggagattacg tctaaaacga
     5341 gttctcgttt aatatatgac ttttaatgaa atatctatgt tcatattcgc tacttaacct
     5401 tccttggcaa gtaatccatc ctattgttaa cattaatata atccatcaag atactttttt
     5461 attaatttaa gtttttcaga attagatgtt ggtgcacctc ctccaaatct agagctgtgg
     5521 aaagaattgg taaaatctta tcgcgatgtt cgtgattctc taaaaattgc agtaaaacat
     5581 ttctagcata ttctttgtta atcaattctt tagaaatatt gcttataaca ggtggctcga
     5641 atgaacctgc tgaatcactt gaagctttgg gcaatatttc actgctcata acactggaca
     5701 gctcgctctc gtgagatttg acactttcct tttgaacctt ttgcttttca atgagttctt
     5761 ttatttcatc ccttgactcc actaactcgt ttccaacact cgagcggccg catcgtgact
     5821 gactgacgat ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     5881 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     5941 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
     6001 gcggagtgta taattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
     6061 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     6121 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     6181 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     6241 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     6301 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     6361 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     6421 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
     6481 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     6541 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     6601 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     6661 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     6721 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa
     6781 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     6841 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     6901 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     6961 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     7021 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     7081 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     7141 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     7201 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     7261 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     7321 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     7381 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     7441 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     7501 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     7561 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     7621 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     7681 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     7741 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     7801 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     7861 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     7921 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     7981 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     8041 ttctccttac gcatctgtgc ggtatttcac accgcataaa ttccgacacc atcgaatggt
     8101 gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga
     8161 atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg
     8221 tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag
     8281 cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac
     8341 agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg
     8401 tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag
     8461 aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca
     8521 gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct
     8581 gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta
     8641 ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc
     8701 agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg
     8761 gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact
     8821 ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca
     8881 ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt
     8941 ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct
     9001 catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca
     9061 gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc
     9121 ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc
     9181 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     9241 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     9301 tttatgcttc cggctcgtat gttgtgt
//
