LOCUS       sequence-178652-        2725 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express csgB curli protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Abdelwahab MT, Kalyoncu E, Onur T, Baykara MZ, Seker UO
  TITLE     Genetically-Tunable Mechanical Properties of Bacterial Functional 
            Amyloid Nanofibers.
  JOURNAL   Langmuir. 2017 Apr 7. doi: 10.1021/acs.langmuir.7b00112.
  PUBMED    28388843
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Langmuir. 
            2017 Apr 7. doi: 10.1021/acs.langmuir.7b00112."
COMMENT     Sequence Label: pZa-tetO-csgB-CmR
FEATURES             Location/Qualifiers
     source          1..2725
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..19
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    26..44
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        join(297..315,316..321,322..338,339..344,345..370)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    297..315
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    322..340
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             405..416
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      896..982
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1017..1034)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1151..1696)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1231..1250)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1810..1904)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1925..2584)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2499..2518
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2585..2687)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacagtcga
       61 cacccaaatc caggaggtga ttggtagtgg tggttaatga aaattaactt actactacca
      121 tatatctcta gatgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact
      181 cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga
      241 actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt ctcgagtccc
      301 tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac atcagcagga
      361 cgcactgacc gaattctacc attcacctct tggatttggg tattaaagag gagaaaggta
      421 ccatgaaaaa caaattgtta tttatgatgt taacaatact gggtgcgcct gggattgcag
      481 ccgcagcagg ttatgattta gctaattcag aatataactt cgcggtaaat gaattgagta
      541 agtcttcatt taatcaggca gccataattg gtcaagctgg gactaataat agtgctcagt
      601 tacggcaggg aggctcaaaa cttttggcgg ttgttgcgca agaaggtagt agcaaccggg
      661 caaagattga ccagacagga gattataacc ttgcatatat tgatcaggcg ggcagtgcca
      721 acgatgccag tatttcgcaa ggtgcttatg gtaatactgc gatgattatc cagaaaggtt
      781 ctggtaataa agcaaatatt acacagtatg gtactcaaaa aacggcaatt gtagtgcaga
      841 gacagtcgca aatggctatt cgcgtgacac aacgttaaac gcgtgctaga ggcatcaaat
      901 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
      961 cgctctcctg agtaggacaa atccgccgcc ctagacctag ggatatattc cgcttcctcg
     1021 ctcactgact cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg
     1081 ggcggagatt tcctggaaga tgccaggaag atacttaaca gggaagtgag agggccgcgg
     1141 caaagccgtt tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa
     1201 atcagtggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggcggct
     1261 ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc
     1321 cgcgtttgtc tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg
     1381 gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg taactatcgt
     1441 cttgagtcca acccggaaag acatgcaaaa gcaccactgg cagcagccac tggtaattga
     1501 tttagaggag ttagtcttga agtcatgcgc cggttaaggc taaactgaaa ggacaagttt
     1561 tggtgactgc gctcctccaa gccagttacc tcggttcaaa gagttggtag ctcagagaac
     1621 cttcgaaaaa ccgccctgca aggcggtttt ttcgttttca gagcaagaga ttacgcgcag
     1681 accaaaacga tctcaagaag atcatcttat taatcagata aaatatttct agatttcagt
     1741 gcaatttatc tcttcaaatg tagcacctga agtcagcccc atacgatata agttgttact
     1801 agtgcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc
     1861 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat
     1921 caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
     1981 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
     2041 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
     2101 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca
     2161 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     2221 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     2281 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     2341 gctcaccgtc tttcattgcc atacggaatt ccggatgagc attcatcagg cgggcaagaa
     2401 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     2461 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
     2521 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
     2581 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
     2641 atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaacg tctcattttc
     2701 gccagatatc gacgtcaaag ggccc
//
