LOCUS       sequence-17871-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  TITLE     T antigen
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBABE-neo largeTgenomic
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             57..581
                     /codon_start=1
                     /product="SV40 (simian virus 40) small t antigen"
                     /label=small t antigen
                     /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
                     GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPECA
                     KKMSANCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLWCDI
                     IGQTTYRDLKL"
     intron          303..648
                     /label=large T intron
                     /note="SV40 (simian virus 40) large T antigen intron"
     CDS             778..798
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttt gcaaagatgg
       61 ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt cttgaaagga
      121 gtgcctgggg gaatattcct ctgatgagaa aggcatattt aaaaaaatgc aaggagtttc
      181 atcctgataa aggaggagat gaagaaaaaa tgaagaaaat gaatactctg tacaagaaaa
      241 tggaagatgg agtaaaatat gctcatcaac ctgactttgg aggcttctgg gatgcaactg
      301 aggtatttgc ttcttcctta aatcctggtg ttgatgcaat gtactgcaaa caatggcctg
      361 agtgtgcaaa gaaaatgtct gctaactgca tatgcttgct gtgcttactg aggatgaagc
      421 atgaaaatag aaaattatac aggaaagatc cacttgtgtg ggttgattgc tactgcttcg
      481 attgctttag aatgtggttt ggacttgatc tttgtgaagg aaccttactt ctgtggtgtg
      541 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
      601 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      661 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      721 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      781 aaaaagaaga gaaaggtaga ag
//
