LOCUS       sequence-178719-        2839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a HR repair template for knock-in a YFP expression cassette 
            at the promoter of human NEAT1 gene. Best used with px330-NEAT1pr_v1
            vector, but also works with px330-NEAT1pr_v2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2839)
  TITLE     Unpublished Fox lab plasmids
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pYFP-NEAT1pr_v1-RT
FEATURES             Location/Qualifiers
     source          1..2839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        696..999
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1000..1203
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1154..1174
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1242..1251
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1248..1250,1251..1253,1254..1964)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1293..1314)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1554..1573)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1901..1922
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1965..2030
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    2154..2275
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2191..2210)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2245..2264
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ggacgctatc agcccgcagc agggtttcct ggccagagaa accgcctgtt ggggtgcgga
       61 gcgttccacg tcccctcgca gcaccccgct ggccttctgg gccgccccgc ccccaacccg
      121 cgcccgggcg ctgacgtcat cggccgagcc cgactcggaa ccaccgcccg aaagtcacgc
      181 gcgcctcccg gcgggacgtg ccctctgcag agatccctcc gccgccgcct ggaattttcc
      241 agatgtcctg ccggcccttc tttcgggcct cggctgggcg tggtgacctc aagcgacccc
      301 ggcggctccc gtcgcccact caagagggcg cccccgcccg ccacgccccc agcccctctc
      361 ggggaagcgc gtcccccgcc cgacctcaac aacatccggg aagaaaaggg gtcttcttcc
      421 tcatggcatt cgcctcccaa atgtcacctt gagcgctgtc cgcgatccca aaaagcactg
      481 ttaaagagaa gcggggatac actggggtcc ttgcgtgggg gccgcctggg agaccatgca
      541 ccgcccggga gtctctccgg gcagggtcgg ggagggactt ttttgccggt ggccgtggag
      601 gaatcgtccc gttgagcaat gaccccggtg acgcggctga gggcatagta atcaattacg
      661 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
      721 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
      781 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
      841 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
      901 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
      961 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     1021 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     1081 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     1141 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     1201 gctggtttag tgaaccgtca gatccgctag cgctaccggt cgccaccatg gtgagcaagg
     1261 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     1321 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     1381 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct
     1441 tcggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct
     1501 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     1561 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     1621 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     1681 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     1741 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     1801 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc
     1861 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     1921 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtccgga ctcagatctc
     1981 gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc accggatcta
     2041 gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     2101 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     2161 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     2221 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaattgt
     2281 ggcaagtcca gccggagtta gcgacaggga gggatgcgcg cctgggtgta gttgtggggg
     2341 aggaagtggc tagctcaggg cttcagggga cagacaggga gagatgactg agttagatga
     2401 gacgaggggg cgggctgggg gtgcgagaag gaagcttggc aaggagacta ggtctagggg
     2461 gaccacagtg gggcaggctg catggaaaat atccgcaggg tcccccaggc agaacagcca
     2521 cgctccaggc caggctgtcc ctactgcctg gtggaggggg aacttgacct ctgggagggc
     2581 gccgctcttg catagctgag cgagcccggg tgcgctggtc tgtgtggaag gaggaaggca
     2641 gggagaggta gaaggggtgg aggagtcagg aggaataggc cgcagcagcc ctggaaatga
     2701 tcaggaaggc aggcagtggg tgcagggctg caggagggcc gggagggcta atcttcaact
     2761 tgtccatgcc agcagcccct ttttttccag accaagggct gtgaacccgc ctggggatga
     2821 ggcctggtct tgtggaact
//
