LOCUS       sequence-178725-        1312 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a HR repair template for knock-in of an EGFP insert that 
            fused to the 5'-end of human SFPQ gene, without disturbing its 
            promoter. Best used with px330-GFP-SFPQ vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Li R, Harvey AR, Hodgetts SI, Fox AH
  TITLE     Functional dissection of NEAT1 using genome editing reveals 
            substantial localisation of the NEAT1_1 isoform outside 
            paraspeckles.
  JOURNAL   RNA. 2017 Mar 21. pii: rna.059477.116. doi: 10.1261/rna.059477.116.
  PUBMED    28325845
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2017 
            Mar 21. pii: rna.059477.116. doi: 10.1261/rna.059477.116."
COMMENT     Sequence Label: pUC57-GFP-SFPQ-RT
FEATURES             Location/Qualifiers
     source          1..1312
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(300..302,303..305,306..1016)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(300..302,303..305,306..1016)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(345..366)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(606..625)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     953..974
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cttcttcgct cactggccaa ctggaaactt cagtcccccc atgcctcccg cctctcaccc
       61 aggggccaat aggaatgatc agaggttcgc aacttagtgt catcaagcat agtaattgca
      121 attggctatt ggagctgtcg atcgtggagt aggcggggcc gtgccagctg gcctatataa
      181 gacgaggaca aaggcggcgc gccgcctgtg tcatccgcca ttttgtgaga agcaaggtgg
      241 cctccacgtt tcctgagcgt cttcttcgct tttgcctcga ccgccccttg accacagaca
      301 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
      361 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
      421 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
      481 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
      541 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
      601 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
      661 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
      721 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
      781 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
      841 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
      901 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
      961 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaaggctg
     1021 cagcggctgc aatgtctcgg gatcggttcc ggagtcgtgg cggtggcggt ggtggcttcc
     1081 acaggcgtgg aggaggcggc ggccgcggcg gcctccacga cttccgttct ccgccgcccg
     1141 gcatgggcct caatcagaat cgcggcccca tgggtcctgg cccgggccag agcggcccta
     1201 agcctccgat cccgccaccg cctccacacc aacagcagca acagccacca ccgcagcagc
     1261 caccgccgca gcagccgcca ccgcatcagc cgccgccgca tccacagccg ca
//
