LOCUS       sequence-178815-        8273 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  contains nrp1b enhancer sequence, and nrp1b specific gene promoter 
            driving expression of eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Quillien A, Abdalla M, Yu J, Ou J, Zhu LJ, Lawson ND
  TITLE     Robust Identification of Developmentally Active Endothelial 
            Enhancers in Zebrafish Using FANS-Assisted ATAC-Seq.
  JOURNAL   Cell Rep. 2017 Jul 18;20(3):709-720. doi: 
            10.1016/j.celrep.2017.06.070.
  PUBMED    28723572
REFERENCE   2  (bases 1 to 8273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.06"; journalName: "Cell Rep"; date: 
            "2017-07-18- 18"; volume: "20"; issue: "3"; pages: "709-720"
COMMENT     Sequence Label: pT2-cryR;nrp1bCE1-P1Egfp
FEATURES             Location/Qualifiers
     source          1..8273
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     620..636
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    656..676
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1271..1295
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2272..2281
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2278..2280,2281..2283,2284..2994)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2278..2280,2281..2283,2284..2994)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2323..2344)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2584..2603)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2931..2952
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2995..3060
                     /label=MCS
                     /note="multiple cloning site"
     protein_bind    complement(3081..3105)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3131..3151)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(3159..3175)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    3191..3325
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(3764..3951,3952..3963)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(3997..4013)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3997..4013)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4050..4070)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4050..4068)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4089..4105)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4102..4124)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4113..4129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4137..4143,4144..4161,4162..4167))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4182..4203
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4320..4337)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4491..5079)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4571..4590)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5250..6041,6042..6110))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5873..5892
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6111..6215)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6378..6399)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(6748..6759,6760..6922)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     polyA_signal    7291..7412
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(7535..8245)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEDDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGHHSTGGMDELYK"
     primer_bind     complement(7615..7638)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(7813..7832)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8082..8100
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      8242..8251
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aactccttac tgtaacttat tgcacctgat gaacaaaaga tgtgaactga acgcaagtag
       61 gaggctaata gaggctagta cagtacaaag atccccaaat gatgtgtgcc ggccttagtg
      121 gaggcagacc cctggttata tacctgtgct gcagaaagag gggcttacaa atggatctct
      181 ctggaatggc attatctcat ggtgggaaca gtgttgtcag cagaatcagc ggggtggact
      241 ctgacctgag aatgacagtc tagcactgaa tggctcagac aatactcttg tggccaacta
      301 tcaccggaaa ataagtgcct gccaaaacaa accagaccag atgaagctac atggctgtag
      361 aacgtcagta tttaatgtgt tgtggccact tggttcacca gatttaagag ctaatttttt
      421 taaatatcac agataatcaa tcgctttttt ttttcttgac tgaaaagctt agactggaaa
      481 attctttgaa aaagtttgca ctacagaaaa taaatacata acatcctgca ctgaatgcac
      541 actattaata gaaaagagag cattattgta ggaaatgtat tgtatatgta cattctgtgc
      601 atttcacaat tttctcgagc aggaaacagc tatgaccatg attacgccaa gctatcaact
      661 ttgtatagaa aagttgggga tgtgctgaaa atgtctctaa cgcacctatg tgacgtgctt
      721 taactaggtt aacgatggtg aacggtcggc acggctcatt aataggcctt cacgtccact
      781 taaagatggc tccatcagaa agagaggtag aagagtcctt gtaacgtcct tctccctcgt
      841 tcctccattt gtcttctagt tttatcttgc tcaagctgaa ggggaagccc tgcgctgcgg
      901 gaccgccgct gttttacctt cgacccaccg ctatatttat cccattgtgc tgaactaatt
      961 gctggtctga catcacctct tgaagaagga cagctgatga acaaagcatc tgcggattat
     1021 ttttagtttg ttcttaagac ggagcttcag ggacacacag gcagagagag agagagacag
     1081 gaagtgctcg tgcagctgga gacaatgtta tcttggtagc aggacaggaa atggggtcca
     1141 atcacttcct ggggcctgag agctttgcta agacactggt ttgagttctg atcggctgtt
     1201 tttccacctc aagggtttca gcacaagggg gtttgagaag tagcttttta gtgctgataa
     1261 tcagatttag tcaagtttgt acaaaaaagc aggctcgcta aatgacatgt gtgatattaa
     1321 ctcaacagtg acatgcttgc tttcctgcac ctattaatgg attaagactg cacatatttt
     1381 ttattttatt ttactatgtg aattgggtaa gcttaactga cagtagtgta tgtgtgtgaa
     1441 tgagagtgta tggatgtttc ccaatgatgg gttgcagctg gaagaataat tattaatatt
     1501 atttagatca gagcaaacta tttttaacta ttagaggaca taccccttcc ttcaccccca
     1561 tatatcttgt tttgatgttt gccagggagg atcaagcatt acttaggggg tcagaccccc
     1621 ccccctcccc ccattcacat tctgacttag agtaaaatat ctaacttgta atgttagtgt
     1681 ttactgaaac cttatttgaa aatgcagtta ttaacatata caacagatgc gtaaacaaac
     1741 atacctcaac ttttttattg acttcttctt aggttgtcac gttaattggg ggtgccaaac
     1801 ccctcatttc tctgcactgc gctcccacgc agaggagcgc gaagtctctc tggtttggcc
     1861 cccggtctcc gtgtctggtt ggcagagaga gacagcgcgt cggtctctgc ctggaagggg
     1921 tgagagggtt tgctcgtttc cagtcctgcc cattttctct caaggaggag gaacgggagc
     1981 aaccagtgct gccgagagtt ttgacaccct cagtcttcat atcggatgct gacaccttcg
     2041 cactagtgag aacctgctaa atatacccag cagtgcccgt gtacgcgcga cacagaggac
     2101 ggaaatcagt catatattag ggagtgaccc gactgctgaa gcgcccgcag acagcgagcc
     2161 gagcccagga cgcgctgtca agcatctcca acacttcgga ttatgaaatt gaagcaaaca
     2221 cagcagtggc ggtgactcta cgagaatctc ccgctttcca gggttgtgat cgccaccatg
     2281 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     2341 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     2401 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     2461 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     2521 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     2581 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     2641 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     2701 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     2761 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     2821 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     2881 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     2941 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtccgga
     3001 ctcagatctc gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc
     3061 accggatcta gataactgat acccagcttt cttgtacaaa gtggggcgcg cctacgtaac
     3121 tagtctcgag caactttatt atacatagtt gataattcac tggccgtcgt tttacggtac
     3181 catcgatgat gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     3241 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     3301 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     3361 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
     3421 tgattatgat cctctagatc agatctgcga agatacggcc acgggtgctc ttgatcctgt
     3481 ggctgatttt ggactgtgct gctcgcagct gctgatgaat cacatacttc ctccattttc
     3541 ttccactgat tgactgttat aatttcccta atttccaggt caaggtgctg tgcattgtgg
     3601 taatagatgt gacatgacgt cacttccaaa ggaccaatga acatgtctga ccaatttcat
     3661 ataatgtgaa aacgattttc ataggcagaa taaataacat ttaaattaaa ctgggcatca
     3721 gcgcaattca attggtttgg taatagcaag ggaaaataga atgaagtgat ctccaaaaaa
     3781 taagtacttt ttgactgtaa ataaaattgt aaggagtaaa aagtactttt ttttctaaaa
     3841 aaatgtaatt aagtaaaagt aaaagtattg atttttaatt gtactcaagt aaagtaaaaa
     3901 tccccaaaaa taatacttaa gtacagtaat caagtaaaat tactcaagta ctttacacct
     3961 ctggttcttg accccctacc ttcagcaagc ccagcagatc cactagttct agagcggccg
     4021 ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg
     4081 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     4141 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     4201 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     4261 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     4321 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     4381 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     4441 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     4501 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     4561 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     4621 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     4681 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     4741 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     4801 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     4861 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     4921 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     4981 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     5041 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     5101 acggggtctg acgctcagtg gaacgaaaac tcacgttaag gcattttggt catgagatta
     5161 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     5221 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     5281 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     5341 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     5401 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     5461 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     5521 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     5581 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     5641 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     5701 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     5761 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     5821 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     5881 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     5941 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     6001 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     6061 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     6121 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     6181 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     6241 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     6301 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     6361 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     6421 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc acgctggcta agaactcatc
     6481 agcctccccg gtccatctac ccacgtacca atgcaccaat tggccacaat gacggctact
     6541 acatggtgcc attccttcct ctttatagga atggagacta cctcctgtcc aacaatgctc
     6601 ttggatacga gtacgcctac ctgttggacc caggtcattg cacaacacca gaaatgccct
     6661 ctgatctgca aaagacgtga atatctgttc agacacccat atccactctg ttccacacag
     6721 gtcagaggtt tgtccaggag ttcttgacag aggtgtaaaa agtactcaaa aattttactc
     6781 aagtgaaagt acaagtactt agggaaaatt ttactcaatt aaaagtaaaa gtatctggct
     6841 agaatcttac ttgagtaaaa gtaaaaaagt actccattaa aattgtactt gagtattaag
     6901 gaagtaaaag taaaagcaag aaagaaaact agagattctt gtttaagctt ttaatctcaa
     6961 aaaacattaa atgaaatgca tacaaggttt tatcctgctt tagaactgtt tgtatttaat
     7021 tatcaaacta taagacagac aatctaatgc cagtacacgc tactcaaagt tgtaaaacct
     7081 cagatttaac ttcagtagaa gctgattctc aaaattgtta gtgtcaagcc tagctctttt
     7141 ggggctgaaa agcaatcctg cagtgctgaa aagcctctca caggcagccg atgcgggaag
     7201 aggtgtatta gtcttgatag agaggctgca aatagcagga aacgtgagca gagactccct
     7261 ggtgtctgaa acacaggccc atttacgcct taagatacat tgatgagttt ggacaaacca
     7321 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     7381 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     7441 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     7501 gtatggctga ttatgatcta gagtcgcggc cgctttactt gtacagctcg tccatgccgc
     7561 cggtggagtg gtggccctcg gcgcgctcgt actgttccac gatggtgtag tcctcgttgt
     7621 gggaggtgat gtccagcttg atgttgacgt tgtaggcgcc gggcagctgc acgggcttct
     7681 tggccttgta ggtggtcttg acctcggcgt cgtagtggcc gccgtccttc agcttcagcc
     7741 tctgcttgat ctcgcccttc agggcgccgt cctcggggta catccgctcg gaggaggcct
     7801 cccagcccat ggtcttcttc tgcattacgg ggccgtcgga ggggaagttg gtgccgcgca
     7861 gcttcacctt gtagatgaac tcgccgtcct gcagggagga gtcctgggtc acggtcacca
     7921 cgccgccgtc ctcgaagttc atcacgcgct cccacttgaa gccctcgggg aaggacagct
     7981 tcaagtagtc ggggatgtcg gcggggtgct tcacgtaggc cttggagccg tacatgaact
     8041 gaggggacag gatgtcccag gcgaagggca gggggccgcc cttggtcacc ttcagcttgg
     8101 cggtctgggt gccctcgtag gggcggccct cgccctcgcc ctcgatctcg aactcgtggc
     8161 cgttcacgga gccctccatg tgcaccttga agcgcatgaa ctccttgatg atggccatgt
     8221 tgtcatcctc gcccttgctc accatggtgg cgaccggtaa tgtcagacct ggt
//
