LOCUS       sequence-178847-        7692 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pPBC-G3-tdT expresses PiggyBac inverted terminal repeat-flanked 
            GCaMP3 and tdTomato under the control of the CAG promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7692)
  AUTHORS   Gee JM, Gibbons MB, Taheri M, Palumbos S, Morris SC, Smeal RM, Flynn
            KF, Economo MN, Cizek CG, Capecchi MR, Tvrdik P, Wilcox KS, White JA
  TITLE     Imaging activity in astrocytes and neurons with genetically encoded 
            calcium indicators following in utero electroporation.
  JOURNAL   Front Mol Neurosci. 2015 Apr 15;8:10. doi: 10.3389/fnmol.2015.00010.
            eCollection 2015.
  PUBMED    25926768
REFERENCE   2  (bases 1 to 7692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Mol 
            Neurosci."; date: "2015-04-15"; volume: "8:10. doi"; pages: " 
            10.3389/fnmol.2015.00010. eCollection 2015"
COMMENT     Sequence Label: pPBC-G3-tdT
FEATURES             Location/Qualifiers
     source          1..7692
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     39..61
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     123..142
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      189..198
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(195..203,204..221,222..224,225..257,258..260,261..284,
                     285..311,312..368,369..374,375..644,645..662,663..1097,
                     1098..1100,1101..1103,1104..1547)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6f
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNTRDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     CDS             join(195..203,204..221,222..224,225..257,258..260,261..284,
                     285..311,312..368,369..374,375..644,645..662,663..1097,
                     1098..1100,1101..1103,1104..1547)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNTRDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      291..300
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     581..602
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(708..729)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(969..988)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    1568..2141
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1591..2143
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1735..1752)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1962..1981
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2158..3588
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     primer_bind     complement(3770..3789)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3814..3861
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     repeat_region   4209..4243
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(4367..4386)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4368..4386)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4473..4490)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4644..5232)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4724..4743)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5403..6194,6195..6263))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6026..6045
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6264..6368)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6436..6454
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6492..6514)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6614..6633
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6714..6732
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     6714..6731
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    6798..6819
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6834..6839,6840..6857,6858..6864)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6872..6888
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6877..6899
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6896..6912
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6896..6912
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        6933..6951
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   7134..7196
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
       61 cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
      121 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagctagcg ctaccggact
      181 cagatctcgc caccatgggt tctcatcatc atcatcatca tggtatggct agcatgactg
      241 gtggacagca aatgggtcgg gatctgtacg acgatgacga taaggatctc gccaccatgg
      301 tcgactcatc acgtcgtaag tggaataaga caggtcacgc agtcagagct ataggtcggc
      361 tgagctcact cgagaacgtc tatatcaagg ccgacaagca gaagaacggc atcaaggcga
      421 acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgcctac cactaccagc
      481 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcgtgc
      541 agtccaaact ttcgaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
      601 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggcggt accggaggga
      661 gcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      721 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggtgagggc gatgccacct
      781 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
      841 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
      901 agcagcacga cttcttcaag tccgccatgc ccgaaggcta catccaggag cgcaccatct
      961 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     1021 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     1081 acaagctgga gtacaacacg cgtgaccaac tgactgaaga gcagatcgca gaatttaaag
     1141 aggctttctc cctatttgac aaggacgggg atgggacaat aacaaccaag gagctgggga
     1201 cggtgatgcg gtctctgggg cagaacccca cagaagcaga gctgcaggac atgatcaatg
     1261 aagtagatgc cgacggtgac ggcacaatcg acttccctga gttcctgaca atgatggcaa
     1321 gaaaaatgaa agacacagac agtgaagaag aaattagaga agcgttccgt gtgtttgata
     1381 aggatggcaa tggctacatc agtgcagcag agcttcgcca cgtgatgaca aaccttggag
     1441 agaagttaac agatgaagag gttgatgaaa tgatcaggga agcagacatc gatggggatg
     1501 gtcaggtaaa ctacgaagag tttgtacaaa tgatgacagc gaagtgagcg gccgcctagc
     1561 ggatctgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
     1621 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     1681 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     1741 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     1801 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     1861 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     1921 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     1981 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     2041 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     2101 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac aacatatatg
     2161 gtgagcaagg gcgaggaggt catcaaagag ttcatgcgct tcaaggtgcg catggagggc
     2221 tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     2281 acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
     2341 ctgtcccccc agttcatgta cggctccaag gcgtacgtga agcaccccgc cgacatcccc
     2401 gattacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     2461 gacggcggtc tggtgaccgt gacccaggac tcctccctgc aggacggcac gctgatctac
     2521 aaggtgaaga tgcgcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacc
     2581 atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag
     2641 atccaccagg ccctgaagct gaaggacggc ggccactacc tggtggagtt caagaccatc
     2701 tacatggcca agaagcccgt gcaactgccc ggctactact acgtggacac caagctggac
     2761 atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgctc cgagggccgc
     2821 caccacctgt tcctggggca tggcaccggc agcaccggca gcggcagctc cggcaccgcc
     2881 tcctccgagg acaacaacat ggccgtcatc aaagagttca tgcgcttcaa ggtgcgcatg
     2941 gagggctcca tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     3001 gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg
     3061 gacatcctgt ccccccagtt catgtacggc tccaaggcgt acgtgaagca ccccgccgac
     3121 atccccgatt acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     3181 ttcgaggacg gcggtctggt gaccgtgacc caggactcct ccctgcagga cggcacgctg
     3241 atctacaagg tgaagatgcg cggcaccaac ttcccccccg acggccccgt aatgcagaag
     3301 aagaccatgg gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag
     3361 ggcgagatcc accaggccct gaagctgaag gacggcggcc actacctggt ggagttcaag
     3421 accatctaca tggccaagaa gcccgtgcaa ctgcccggct actactacgt ggacaccaag
     3481 ctggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga gcgctccgag
     3541 ggccgccacc acctgttcct gtacggcatg gacgagctgt acaagtaagc ggccgcttcg
     3601 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
     3661 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     3721 gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga
     3781 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc
     3841 acggtgttgg gtcgtttgtt cggcgcgcca tcgaagcagc tccagcctac acaatcgctc
     3901 aagacgtgta atgcttttat tatatattag tcacgatatc tataacaaga aaatatatat
     3961 ataataagtt atcacgtaag tagaacatga aataacaata taattatcgt atgagttaaa
     4021 tcttaaaagt cacgtaaaag ataatcatgc gtcattttga ctcacgcggt cgttatagtt
     4081 caaaatcagt gacacttacc gcattgacaa gcacgcctca cgggagctcc aagcggcgac
     4141 tgagatgtcc taaatgcaca gcgacggatt cgcgctattt agaaagagag agcaatattt
     4201 caagaatgca tgcgtcaatt ttacgcagac tatctttcta gggttaaaaa agatttgcgc
     4261 tttactcgac ctaaacttta aacacgtcat agaatcttcg tttgacaaaa accacattgt
     4321 ggggtaccga gctcgaattc atcgatgata tcagatctgc cggtctccct atagtgagtc
     4381 gtattaattt cgataagcca ggttaacctg cattaatgaa tcggccaacg cgcggggaga
     4441 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     4501 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     4561 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     4621 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     4681 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     4741 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     4801 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     4861 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4921 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4981 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     5041 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     5101 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     5161 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     5221 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     5281 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     5341 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     5401 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     5461 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     5521 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     5581 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     5641 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     5701 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     5761 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     5821 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     5881 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     5941 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     6001 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     6061 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     6121 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     6181 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     6241 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     6301 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     6361 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     6421 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
     6481 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     6541 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     6601 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat ggacatattg
     6661 tcgttagaac gcggctacaa ttaatacata accttatgta tcatacacat acgatttagg
     6721 tgacactata gaactcgacc tcgaggctgg cacgacaggt ttcccgactg gaaagcgggc
     6781 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     6841 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     6901 aacagctatg accatgatta cgccaagctc gaaattaacc ctcactaaag ggaacaaaag
     6961 ctggagctcg tctttgatca aaacgcaaat cgacgaaaat gtgtcggaca atatcaagtc
     7021 gatgagcgaa aaactaaaaa ggctagaata cgacaatctc acagacagcg ttgagatata
     7081 cggtattcac gacagcaggc tgaataataa aaaaattaga aactattatt taaccctaga
     7141 aagataatca tattgtgacg tacgttaaag ataatcatgc gtaaaattga cgcatgtgtt
     7201 ttatcgatct gtatatcgag gtttatttat taatttgaat agatattaag ttttattata
     7261 tttacactta catactaata ataaattcaa caaacaattt atttatgttt atttatttat
     7321 taaaaaaaaa caaaaactca aaatttcttc tataaagtaa caaaactttt aaacattctc
     7381 tcttttacaa aaataaactt attttgtact ttaaaaacag tcatgttgta ttataaaata
     7441 agtaattagc ttaacttata cataatagaa acaaattata cttattagac attgattatt
     7501 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     7561 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     7621 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     7681 tcaatgggtg ga
//
