LOCUS       sequence-178879-        5076 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration vector at an attB site in Bacteroides; contains the 
            P1T_DP aTC-inducible promoter with the RBS GH023.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   Lim B, Zimmermann M, Barry NA, Goodman AL
  TITLE     Engineered Regulatory Systems Modulate Gene Expression of Human 
            Commensals in the Gut.
  JOURNAL   Cell. 2017 Apr 20;169(3):547-558.e15. doi: 
            10.1016/j.cell.2017.03.045.
  PUBMED    28431252
REFERENCE   2  (bases 1 to 5076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.03"; journalName: "Cell"; date: "2017-04-20- 
            20"; volume: "169"; issue: "3"; pages: "547-558.e15"
COMMENT     Sequence Label: pNBU2_erm_P1T_DP-GH023
FEATURES             Location/Qualifiers
     source          1..5076
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            102..211
                     /note="incP origin of transfer"
     rep_origin      382..770
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     protein_bind    885..903
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    947..965
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             join(4187..4255,4256..5047)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4405..4424)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 caattcgagg gggatcaatt ccgtgatagg tgggctgccc ttcctggttg gcttggtttc
       61 atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc
      121 aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct
      181 cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa
      241 gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga
      301 aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaaacggaa ttgatccggc
      361 cacgatgcgt ccggcgtaga ggatctgaag atcagcagtt caacctgttg atagtacgta
      421 ctaagctctc atgtttcacg tactaagctc tcatgtttaa cgtactaagc tctcatgttt
      481 aacgaactaa accctcatgg ctaacgtact aagctctcat ggctaacgta ctaagctctc
      541 atgtttcacg tactaagctc tcatgtttga acaataaaat taatataaat cagcaactta
      601 aatagcctct aaggttttaa gttttataag aaaaaaaaga atatataagg cttttaaagc
      661 ttttaaggtt taacggttgt ggacaacaag ccagggatgt aacgcactga gaagccctta
      721 gagcctctca aagcaatttt gagtgacaca ggaacactta acggctgaca tgggaattcc
      781 cctccaccgc ggtggctttt gcacccgctt tccaagagaa gaaagccttg ttaaattgac
      841 ttagtgtaaa agcgcagtac tgcttgacca taagaacaaa aaaatctcta tcactgatag
      901 ggataaagtt tggaagataa agctaaaagt tcttatcttt gcagtctccc tatcagtgat
      961 agagacgaaa taaagacata taaaagaaaa gacaccatgg tgcaggaatt cgatatcaag
     1021 cttatcgata ccgtcgacta attgcctatc ttccagtgat ggaacagcat ttgtgcattg
     1081 gctgcaacaa tcagccttac ttgtgcctgt tctatttccg aaccgaccgc ttgtatgaat
     1141 gcatcaaaat tcgttttctc tacgttggat tccttgttgc tcatattgtg atgataattt
     1201 ctacaaatat agtcattggt aactatctat gaaactgttt gatactttta tagttgatta
     1261 aacttgttca tggcatttgc cttaatatca tccgctatgt caatgtaggg tttcatagct
     1321 ttgtagtcgc tgtgtcccgt ccatttcatg accacctgtg ccgggattcc gagagccagc
     1381 gcattgcaga tgaatgtcct ttttcctgca tgggtactga gcaaagcgta tttgggtgtg
     1441 acttcatcaa tacgttcatt tcccttgtag taggtttccc gtacaggctc gttgatttct
     1501 gccagttcgc ccagctcttt caggtaatcg ttcatcttct ggttgctgat gacgggcaga
     1561 gccatgtaat tctcgaaatg gatgtccttg tatttgtcca gtatggcttt gctgtatttg
     1621 ttcagttcaa tcgtcaggct gtcggcagtc ttgactgtgg ttatttcgat gtggtcggac
     1681 ttcacatcgc ttcttttcag attgcgaaca tccgaatacc gcaaactcgt aaagcagcag
     1741 aacaggaaaa catcacgcac acgttccagg tattgcttat ccttgggtat ctggtagtct
     1801 ttcagcttgt tcagttcatc ccaagtcagg aagattactt ttttcgaggt ggttttcagt
     1861 ttcggtttga acgtatcgta tgcaatgttc tgatgatgtc ctttcttgaa gctccagcgc
     1921 aggaaccatt tgaggaatcc catttgcttg ccgatggtgc tgtttctcat atccttggtg
     1981 tcacgcagga agttgacgta ttcgttcaat ccaaactcgt tgaaatagtt gaacgttgca
     2041 tcctccttga actctttgag gtggttcctc actgctgcaa atttttcata ggtggatgcc
     2101 gtccagttat tctggttacc gcactctttt acaaactcat cgaacacctc ccaaaagctg
     2161 acaggggctt cttccggctg ttcttcactg gtgtctttca ttctcatgtt gaaagcttcc
     2221 ttcaactgtt gggtcgttgg catgacctcc tgcacctcaa attccttgaa aatattctgg
     2281 atttcggcat agtatttcag caagtccgta ttgatttcgg ctgcactttg ctttagcttg
     2341 ttggtacatc cgttctttac ccgctgctta tctgcatccc atttggctac gtcaatccgg
     2401 tagcccgttg taaactcgat acgttggctg gcaaagatga cacgcatacg gatgggtacg
     2461 ttctctacga ttggcacacc gttctttttc cggctctcca atgcaaaaat gatgttgcgc
     2521 ttgatattca taattgggtg cgtttgaaat tctacaccca aatatacacc caattattga
     2581 gatagcaaaa gacatttaga aacatttact tttactctat attgtaattt acacttgatt
     2641 atcagtcgtt tgcagtttta tgatattctg tgaaagtata agttcgagag cctgtctctc
     2701 cgcaaaaaac gctgaaaatc agcagattgc aaaacaaaca ccctgtttta cacccaagaa
     2761 tgtaaagtcg ggtgtttttg ttttatttaa gataatacaa ccactacata ataaaagagt
     2821 agcgatatta aaagaatccg atgagaaaag actaatattt atctatccat tcagtttgat
     2881 ttctcaggac tttacatcgt cctgaaagta tttgttggta ccgaggacgc gtaaacattt
     2941 acagttgcat gtggcctatt gtttttagcc gttaaatatt ttataactat taaatagcga
     3001 tacaaattgt tcgaaactaa tattgtttat atcatatatt ctcgcatgtt ttaaagcttt
     3061 attaaattga ttttttgtaa acagtttttc gtactctttg ttaacccatt tcattacaaa
     3121 agtttcatat ttttttctct ctttaaatgc catttttgct ggctttcttt ttaatacaat
     3181 taatgtgcta tccactttag gttttggatg gaaataatac ctaggaattt ttgctaatat
     3241 agaaatatct acctctgcca ttaacagcaa tgctagtgat ctgtttgtat ctaataacat
     3301 tttagcaaaa ccatattcca ctattaaata acttattgtg gctgaacttt caaaaacaat
     3361 ttttcgaatt atatttgtgc ttatgttgta aggtatgctg ccaaatattt tatatggatt
     3421 gtggctagga aatgtaaatt tcagtatatc atcatttact atttgatagt taggataatt
     3481 taagagctta ttacgagtta cctcacataa tttagaatca atttctatcg ccgttacaaa
     3541 attacatctc tttaccaatc cagcagtaaa atgacctttc cctgcaccta tttcaaagat
     3601 gttatctttt tcatctaaac ttatgcaatt cattattttt tctatgtgat attttgaagt
     3661 aataaaattt tgactatctt ttatatttac tttgttcatt ataacctctc cttaatttat
     3721 tgcatctctt ttcgaatatt tatgtttttt gagaaaagaa cgtactcatg gttcatcccg
     3781 atatgcgtat cggtctgtat atcagcaact ttctatgtgt ttcaactaca atagtcatct
     3841 attctcatct ttctgagtcc accccctgca aagcccctct ttacgacata aaaattcggt
     3901 cggaaaaggt atgcaaaaga tgtttctctc tttaagagaa actcttcggg atgcaaaaat
     3961 atgaaaataa ctccaattca ccaaattata tagcgacttt tttacaaaat gctaaaattt
     4021 gttgatttcc gtcaagcaat tgttgagcaa aaatgtcttt tacgataaaa tgatacctca
     4081 atatcaactg tttagcaaaa cgatatttct cttaaagaga gaaacacctt tttgttcacc
     4141 aatccccgac ttttaatccc gcggccatga ttgaaaaagg aagagtatga gtattcaaca
     4201 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     4261 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     4321 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     4381 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     4441 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     4501 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     4561 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     4621 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     4681 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     4741 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     4801 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     4861 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     4921 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     4981 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     5041 ttggtaactg tcagaccaag tttactcata acgcgt
//
