LOCUS       sequence-179036-        4571 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses AMPK alpha1  in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Schaffer BE, Levin RS, Hertz NT, Maures TJ, Schoof ML, Hollstein PE,
            Benayoun BA, Banko MR, Shaw RJ, Shokat KM, Brunet A
  TITLE     Identification of AMPK Phosphorylation Sites Reveals a Network of 
            Proteins Involved in Cell Invasion and Facilitates Large-Scale 
            Substrate Prediction.
  JOURNAL   Cell Metab. 2015 Nov 3;22(5):907-21. doi: 
            10.1016/j.cmet.2015.09.009. Epub 2015 Oct 8.
  PUBMED    26456332
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2015.09.009"; journalName: "Cell Metab"; date: 
            "2015-11-3- 3"; volume: "22"; issue: "5"; pages: "907-21"
COMMENT     Sequence Label: pECE HA AMPKa1 WT
FEATURES             Location/Qualifiers
     source          1..4571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             320..346
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     polyA_signal    2129..2263
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2166..2185)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2220..2239
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2322..2341)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2606..2623)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2777..3365)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2857..2876)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3536..4327,4328..4396))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4159..4178
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4397..4501)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(4556..4571,1..5))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
       61 ttagtcagca aggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
      121 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
      181 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
      241 aggccgcctc ggcctctgag ctattccaga agtagtgaag aggctttttt ggaggagatc
      301 taagcttgcc gccaccatgt atgatgttcc tgattatgct agcctcccgg ggaattcggc
      361 gacagccgag aagcagaaac acgacgggcg ggtgaagatc ggccactaca ttctgggtga
      421 cacgctgggg gtcggcacct tcggcaaagt gaaggttggc aaacatgaat tgactgggca
      481 taaagtagct gtgaagatac tcaatcgaca gaagattcgg agccttgatg tggtaggaaa
      541 aatccgcaga gaaattcaga acctcaagct tttcaggcat cctcatataa ttaaactgta
      601 ccaggtcatc agtacaccat ctgatatttt catggtgatg gaatatgtct caggaggaga
      661 gctatttgat tatatctgta agaatggaag gctggatgaa aaagaaagtc ggcgtctgtt
      721 ccaacagatc ctttctggtg tggattattg tcacaggcat atggtggtcc atagagattt
      781 gaaacctgaa aatgtcctgc ttgatgcaca catgaatgca aagatagctg attttggtct
      841 ttcaaacatg atgtcagatg gtgaattttt aagaacaagt tgtggctcac ccaactatgc
      901 tgcaccagaa gtaatttcag gaagattgta tgcaggccca gaggtagata tatggagcag
      961 tggggttatt ctctatgctt tattatgtgg aacccttcca tttgatgatg accatgtgcc
     1021 aactcttttt aagaagatat gtgatgggat cttctatacc cctcaatatt taaatccttc
     1081 tgtgattagc cttttgaaac atatgctgca ggtggatccc atgaagaggg ccacaatcaa
     1141 agatatcagg gaacatgaat ggtttaaaca ggaccttcca aaatatctct ttcctgagga
     1201 tccatcatat agttcaacca tgattgatga tgaagcctta aaagaagtat gtgaaaagtt
     1261 tgagtgctca gaagaggaag ttctcagctg tctttacaac agaaatcacc aggatccttt
     1321 ggcagttgcc taccatctca taatagataa caggagaata atgaatgaag ccaaagattt
     1381 ctatttggcg acaagcccac ctgattcttt tcttgatgat catcacctga ctcggcccca
     1441 tcctgaaaga gtaccattct tggttgctga aacaccaagg gcacgccata cccttgatga
     1501 attaaatcca cagaaatcca aacaccaagg tgtaaggaaa gcaaaatggc atttaggaat
     1561 tagaagtcaa agtcgaccaa atgatattat ggcagaagta tgtagagcaa tcaaacaatt
     1621 ggattatgaa tggaaggttg taaacccata ttatttgcgt gtacgaagga agaatcctgt
     1681 gacaagcact tactccaaaa tgagtctaca gttataccaa gtggatagta gaacttatct
     1741 actggatttc cgtagtattg atgatgaaat tacagaagcc aaatcaggga ctgctactcc
     1801 acagagatcg ggatcagtta gcaactatcg atcttgccaa aggagtgatt cagatgctga
     1861 ggctcaagga aaatcctcag aagtttctct tacctcatct gtgacctcac ttgactcttc
     1921 tcctgttgac ctaactccaa gacctggaag tcacacaata gaattttttg agatgtgtgc
     1981 aaatctaatt aaaattcttg cacaataatc tagataagta atgatcataa tcagccatat
     2041 cacatctgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     2101 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     2161 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     2221 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcctctacg ccggacgcat
     2281 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
     2341 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat
     2401 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct
     2461 tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc
     2521 gcataaggga gagcgtcgat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     2581 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     2641 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     2701 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     2761 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     2821 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     2881 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     2941 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3001 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3061 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3121 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3181 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     3241 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3301 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3361 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     3421 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     3481 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     3541 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     3601 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     3661 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     3721 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     3781 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     3841 ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     3901 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     3961 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     4021 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     4081 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     4141 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     4201 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     4261 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     4321 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     4381 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     4441 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     4501 gcacatttcc ccgaaaagtg ccacctgacc ttctgaggcg gaaagaacca gctgtggaat
     4561 gtgtgtcagt t
//
