LOCUS       sequence-179085-        7513 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SOBIR1 split YFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7513)
  AUTHORS   Postma J, Liebrand TW, Bi G, Evrard A, Bye RR, Mbengue M, Kuhn H, 
            Joosten MH, Robatzek S
  TITLE     Avr4 promotes Cf-4 receptor-like protein association with the 
            BAK1/SERK3 receptor-like kinase to initiate receptor endocytosis and
            plant immunity.
  JOURNAL   New Phytol. 2016 Apr;210(2):627-42. doi: 10.1111/nph.13802. Epub 
            2016 Jan 14.
  PUBMED    26765243
REFERENCE   2  (bases 1 to 7513)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.13802";
            journalName: "New Phytol"; date: "2016-04"; volume: "210"; issue: 
            "2"; pages: "627-42"
COMMENT     Sequence Label: p35S::AtSOBIR1-YFPc
FEATURES             Location/Qualifiers
     source          1..7513
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..345
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     347..363
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     348..364
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    380..404
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2364..2388)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2399..2650
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     2587..2608
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2942..2966
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      3078..3787
                     /label=oriV
                     /note="incP origin of replication"
     promoter        3860..3951
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3952..4020,4021..4812)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4170..4189)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4983..5571
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5472..5491
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    5883..5907
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(6001..6253)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6273..6824)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(6866..7045)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcgaaagga cagtagaaaa ggaagatggc ttctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ctctaccgac agtggtccca aagatggacc
      181 cccacccacg aggaacatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacctcga ggtcgacggt
      361 atcgataagc ttcccatcaa caagtttgta caaaaaagca ggctccgcgg ccgccccctt
      421 caccatggct gttcccacgg gaagcgccaa tctctttctc cggcctctca tcctcgccgt
      481 tctctccttt cttcttctct cttcattcgt ctcctctgtt gagtggctcg atatcgattc
      541 ctcagatctc aaagctcttc aggtcatcga aacagagctc ggagtcaaca gtcaacgttc
      601 ttcggccagt gatgtcaacc cttgcggtcg ccgaggagtt ttctgcgaga gacggcactc
      661 tgccaccacc ggagaatacg ttctccgtgt cacgaggctc gtctaccgat ccaggagctt
      721 gacggggact atctctccgg tcatcggaat gttgtcggag ctcaaggaac ttactttgtc
      781 taataaccag ttggtcaacg ctgttccggt ggatatctta agctgtaagc aacttgaagt
      841 tcttgatctt cggaaaaata gattttccgg tcagattccg ggaaactttt ctagtttgag
      901 ccgtcttcga atccttgatc tctcttcgaa caagttatcc gggaacttga atttcttgaa
      961 gaatctacgc aatctggaga atctctctgt tgcaaacaat ctcttctcag gcaaaatccc
     1021 ggaacaaatt gtgtcgttcc acaatctccg gttctttgat ttctccggaa accggtattt
     1081 ggaaggtccg gctccggtta tgagcagtat caagcttcag acatctccac atcaaacaag
     1141 acacattctt gctgaaactc caacttcaag tcccacgaac aaacccaaca acagcactac
     1201 atcaaaagct ccaaagggag ctccaaaacc agggaagttg aagaagaaga agaagaagag
     1261 caagaagaag aaagtagcgg catggatctt agggtttgtc gttggagcca taggaggaac
     1321 aatctctggg tttgtcttct cggtgttgtt caaattgatt attcaagcaa taagaggatc
     1381 agaaaaacca ccaggtcctt ccatattcag cccattgatc aagaaagctg aagatttagc
     1441 tttcttggag aatgaagaag cgttagcttc tctggagatc ataggaagag gaggttgtgg
     1501 agaagttttc aaagctgaac taccaggaag caatgggaag atcatagctg tgaagaaagt
     1561 gatccaaccg cctaaagacg ccgatgaact aacagatgaa gattccaagt ttctgaacaa
     1621 gaaaatgagg caaattagat ccgagatcaa cacagtcggc catatccggc accggaatct
     1681 tctcccattg ttagcacacg tgtcaagacc cgagtgccac tacctcgttt acgagtacat
     1741 ggaaaagggg agtttgcaag atatattgac tgacgtacaa gctggaaatc aagaactaat
     1801 gtggccagcg aggcacaaga ttgctttagg tatagctgca gggcttgagt accttcacat
     1861 ggatcataac ccacgaatca ttcacagaga cttaaagcca gccaatgttc ttcttgatga
     1921 tgacatggaa gccagaattt cagatttcgg actggcaaag gcaatgccag atgcagtcac
     1981 acatattaca acctcgcatg ttgcaggtac tgtgggatac atagcaccag agttttatca
     2041 aacccacaag ttcacagata aatgtgatat ctatagtttt ggagtgattc ttgggattct
     2101 ggtgattggg aagcttcctt cagatgagtt ttttcagcat actgacgaga tgagtctgat
     2161 aaagtggatg aggaacataa taacatcgga aaatcctagc cttgcgattg atccgaagtt
     2221 gatggatcaa ggattcgatg aacagatgct tctggttctg aagatcgcct gttactgtac
     2281 tttggatgat ccaaaacaga ggccgaacag taaagatgtc aggaccatgt tgtcccagat
     2341 caagcacaag ggtgggcgcg ccgacccagc tttcttgtac aaagtggttg atgggatgga
     2401 caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcgg
     2461 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     2521 gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg
     2581 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     2641 gctgtacaag taatctagag tccgcaaaaa tcaccagtct ctctctacaa atctatctct
     2701 ctctattttt ctccagaata atgtgtgagt agttcccaga taagggaatt agggttctta
     2761 tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa
     2821 aatacttcta tcaataaaat ttctaattcc taaaaccaaa atccagtgac cgggcggccg
     2881 ccaccgcggt ggagggggat cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt
     2941 ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga
     3001 tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgta catcaccgac
     3061 gagcaaggca agaccgagcg cctttccgac gctcaccggg ctggttgccc tcgccgctgg
     3121 gctggcggcc gtctatggcc ctgcaaacgc gccagaaacg ccgtcgaagc cgtgtgcgag
     3181 acaccggccg ccggcgttgt ggatacctcg cggaaaactt ggccctcact gacagatgag
     3241 gggcggacgt tgacacttga ggggccgact cacccggcgc ggcgttgaca gatgaggggc
     3301 aggctcgatt tcggccggcg acgtggagct ggccagcctc gcaaatcggc gaaaacgcct
     3361 gattttacgc gagtttccca cagatgatgt ggacaagcct ggggataagt gccctgcggt
     3421 attgacactt gaggggcgcg actactgaca gatgaggggc gcgatccttg acacttgagg
     3481 ggcagagtgc tgacagatga ggggcgcacc tattgacatt tgaggggctg tccacaggca
     3541 gaaaatccag catttgcaag ggtttccgcc cgtttttcgg ccaccgctaa cctgtctttt
     3601 aacctgcttt taaaccaata tttataaacc ttgtttttaa ccagggctgc gccctgtgcg
     3661 cgtgaccgcg cacgccgaag gggggtgccc ccccttctcg aaccctcccg gcccgctaac
     3721 gcgggcctcc catcccccca ggggctgcgc ccctcggccg cgaacggcct caccccaaaa
     3781 atggcagcgc tggcagtcct tgccattgcc gggatcgggg cagtaacggg atgggcgatc
     3841 agcccgaaag ctacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     3901 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     3961 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     4021 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     4081 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4141 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     4201 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     4261 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     4321 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     4381 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     4441 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     4501 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     4561 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     4621 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     4681 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     4741 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     4801 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     4861 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     4921 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     4981 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5041 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5101 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     5161 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     5221 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     5281 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     5341 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     5401 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     5461 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     5521 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     5581 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat ggactctagc
     5641 tagaggatca caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga
     5701 gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac
     5761 atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg
     5821 ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct
     5881 ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg
     5941 cggacgtttt taatgatcga atactaacgt ctctaccaga tatcagcttg catgccggtc
     6001 gatctagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg cgctatattt
     6061 tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa acccatctca
     6121 taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc aacagaaatt
     6181 atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt tattgccaaa
     6241 tgtttgaacg atctgcttga ctctaggggt catcagattt cggtgacggg caggaccgga
     6301 cggggcggca ccggcaggct gaagtccagc tgccagaaac ccacgtcatg ccagttcccg
     6361 tgcttgaagc cggccgcccg cagcatgccg cggggggcat atccgagcgc ctcgtgcatg
     6421 cgcacgctcg ggtcgttggg cagcccgatg acagcgacca cgctcttgaa gccctgtgcc
     6481 tccagggact tcagcaggtg ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg
     6541 ggggagacgt acacggtcga ctcggccgtc cagtcgtagg cgttgcgtgc cttccaggga
     6601 cccgcgtagg cgatgccggc gacctcgccg tccacctcgg cgacgagcca gggatagcgc
     6661 tcccgcagac ggacgaggtc gtccgtccac tcctgcggtt cctgcggctc ggtacggaag
     6721 ttgaccgtgc ttgtctcgat gtagtggttg acgatggtgc agaccgccgg catgtccgcc
     6781 tcggtggcac ggcggatgtc ggccgggcgt cgttctgggc tcatggtaga tcccctcgat
     6841 cgagttgaga gtgaatatga gactctaatt ggataccgag gggaatttat ggaacgtcag
     6901 tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg acttttgaac
     6961 gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa cccgcggctc
     7021 agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct tgtcccgcgt
     7081 catcggcggg ggtcataacg tgactccctt aattctccgc tcatgatcag attgtcgttt
     7141 cccgccttcg gtttgggcgc gcccggttca gaagaccaga gggctattga gacttttcaa
     7201 caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc
     7261 gaaaggacag tagaaaagga agatggcttc tacaaatgcc atcattgcga taaaggaaag
     7321 gctatcgttc aagatgcctc taccgacagt ggtcccaaag atggaccccc acccacgagg
     7381 aacatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat
     7441 acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag
     7501 accagagggc tat
//
