LOCUS       sequence-17960-e         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Meister G, Landthaler M, Peters L, Chen PY, Urlaub H, Luhrmann R, 
            Tuschl T
  TITLE     Identification of novel argonaute-associated proteins.
  JOURNAL   Curr Biol. 2005 Dec 6;15(23):2149-55. doi: 
            10.1016/j.cub.2005.10.048. Epub 2005 Nov 10.
  PUBMED    16289642
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2005.10.048"; journalName: "Curr Biol"; date: 
            "2005-12-6- 6"; volume: "15"; issue: "23"; pages: "2149-55"
COMMENT     Sequence Label: pFLAG/HA-TNRC6B
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             42..65
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             66..92
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    108..132
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atcatcgata gtgtggtgga attctgatcg ccgccgccat ggactacaag gacgacgatg
       61 acaagtaccc ttatgacgtg cccgattacg ctttgaacca gatatcaaca agtttgtaca
      121 aaaaagcagg ctccaccatg ggaaccaatt cagtcgacat gagagagaag gagcaagaaa
      181 gggaagaaca gttaatggaa gacaagaaaa ggaagaaaga ggataaaaag aaaaaagaag
      241 ccactcagaa ggtcacggaa caaaaaacca aagtgcccga agtgacgaaa ccaagtttaa
      301 gccaaccaac ggccgccagc ccaattggca gctctccatc gccaccagtc aatggtggca
      361 acaatgccaa aagggtggca gtgccgaacg gacaaccgcc aagcgccgcc cgctacatgc
      421 ctcgggaggt gccgccgcga ttccgttgcc agcaggacca caaagtgtta ctaaaacgtg
      481 ggcagccccc tccaccgtcc tgcatgctcc ttgggggntg gggcagggcc tcctccctgc
      541 acagcacctg gagcaaaccc aaacaacgca caagtgacag gagcgctgct gcagagtgag
      601 agtgggactg cgccagactc aacccttgga ggtgctgctg cttcaaatta tgcaaattcc
      661 acttggggct cgggagcctc ctccacaacg gcacctcccc caacccat
//
