LOCUS       sequence-17975-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  TITLE     Unpublished, Paco
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pYC6Lys-TRP1URA3
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..25
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6..24
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             44..715
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
ORIGIN
        1 agctgtaata cgactcacta tagggaatat taagctcgcc cttatgtcng ttattaattt
       61 cacaggtagt tctggtccat tggtgaaagt ttgcggcttg cagagcacag aggccgcaga
      121 atgtgctcta gattccgatg ctgacttgct gggtattata tgtgtgccca atagaaagag
      181 aacaattgac ccggttattg caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag
      241 ttcaggcact ccgaaatact tggttggcgt gtttcgtaat caacctaagg aggatgtttt
      301 ggctctggtc aatgattacg gcattgatat cgtccaactg catggagatg agtcgtggca
      361 agaataccaa gagttcctcg gtttgccagt tattaaaaga ctcgtatttc caaaagactg
      421 caacatacta ctcagtgcag cttcacagaa acctcattcg tttattccct tgtttgattc
      481 agaagcaggt gggacaggtg aacttttgga ttggaactcg atttctgact gggttggaag
      541 gcaagagagc cccgaaagct tacattttat gttagctggt ggactgacgc cagaaaatgt
      601 tggtgatgcg cttagattaa atggcgttat tggtgttgat gtaagcggag gtgtggagac
      661 aaatggtgta aaagactcta acaaaatagc aaatttcgtc aaaaatgcta agaaaaaggg
      721 cgagcttcga ggtcacccat cgaaagctac atataaggaa cgtgctgcta ctcatcctag
      781 tcctgttgct gccaagctat ttaatatcat g
//
