LOCUS       sequence-179825-        1043 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  titin in DNA-binding domain (BD) vector, used for yeast-two hybrid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1043)
  TITLE     Shiang Lab CMYA5 project plasmids (unpublished)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: TTN/pAS2-1
FEATURES             Location/Qualifiers
     source          1..1043
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      837..1024
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(892..916)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 gccggtattg caatacccag ctttgactca tatggccatg ggctccagaa atgtatactc
       61 ccttgaaatc cgaaatgcat cagtcagcga cagtggaaag tacacaatta aggccaaaaa
      121 tttccgtggc cagtgttcag ctacagcttc cttaatggtc cttcctctag ttgaagaacc
      181 ttccagagag gtagtattga gaacaagtgg tgacacaagc ttgcaaggaa gcttctcgtc
      241 tcagtcagtc caaatgtctg cctccaagca ggaggcctcc ttcagcagtt tcagcagcag
      301 cagtgctagc agcatgactg agatgaaatt tgcaagcatg tctgcccaaa gcatgtcctc
      361 catgcaagag tcctttgtag aaatgagttc cagcagcttt atgggaatat ctaatatgac
      421 acaactggaa agctcaacta gtaaaatgct taaagcaggc ataagaggaa ttccgcctaa
      481 aattgaagct cttccatctg atatcagcat tgatgaaggc aaagttctaa cagtagcctg
      541 tgctttcacg ggtgagccta ccccagaagt aacatggtcc tgtggtggaa gaaaaatcca
      601 cagtcaagaa caggggaggt tccacattga aaacacagat gacctgacaa ccctgatcat
      661 catggacgta cagaaacaag atggtggact ttataccctg agtttaggga atgaatttgg
      721 atctgactct gccactgtga atatacatat tcgatccatt taagagggcc tgtgcgaatt
      781 caatcgaatt cctgcagccc gggggatccg tcgacctgca gccaagctaa ttccgggcga
      841 atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca
      901 aattttaaag tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc
      961 tgtaggtcag gttgctttct caggtatagc atgaggtcgc tcttattgac cacacctcta
     1021 ccggcatgca agcttggcgt aat
//
