LOCUS       sequence-179968-        7796 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Syntaxin17 tagged with Turquoise2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7796)
  AUTHORS   Tsuboyama K, Koyama-Honda I, Sakamaki Y, Koike M, Morishita H, 
            Mizushima N
  TITLE     The ATG conjugation systems are important for degradation of the 
            inner autophagosomal membrane.
  JOURNAL   Science. 2016 Nov 25;354(6315):1036-1041. Epub 2016 Oct 20.
  PUBMED    27885029
REFERENCE   2  (bases 1 to 7796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-11-25"; volume: "354(6315)"; pages: "1036-1041. Epub 
            2016 Oct 20"
COMMENT     Sequence Label: pMRXIP Turquoise2-Syntaxin17
FEATURES             Location/Qualifiers
     source          1..7796
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    11..368
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             433..849
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     779..801
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     817..833
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    859..1233
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1171..1187
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             join(1250..1252,1253..1255,1256..1966)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1295..1316)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1556..1575)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1903..1924
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2991..3557
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3007..3559
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3151..3168)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3378..3397
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3571..4170
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3571..3590)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4067..4087
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4394..4863
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4993..5010)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5164..5752)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5244..5263)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5916..6707,6708..6776))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6539..6558
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6777..6881)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6949..6967
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        7069..7448
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 accgggaggt aagctggcca gcaacttatc tgtgtctgtc cgattgtcta gtgtctatga
       61 ctgattttat gcgcctgcgt cggtactagt tagctaacta gctctgtatc tggcggaccc
      121 gtggtggaac tgacgagttc ggaacacccg gccgcaaccc tgggagacgt cccagggact
      181 tcgggggccg tttttgtggc ccgacctgag tccaaaaatc ccgatcgttt tggactcttt
      241 ggtgcacccc ccttagagga gggatatgtg gttctggtag gagacgagaa cctaaaacag
      301 ttcccgcctc cgtctgaatt tttgctttcg gtttgggacc gaagccgcgc cgcgcgtctt
      361 gtctgctgca gcatcgttct gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa
      421 aatatgggcc cgggccagac tgttaccact cccttaagtt tgaccttagg tcactggaaa
      481 gatgtcgagc ggatcgctca caaccagtcg gtagatgtca agaagagacg ttgggttacc
      541 ttctgctctg cagaatggcc aacctttaac gtcggatggc cgcgagacgg cacctttaac
      601 cgagacctca tcacccaggt taagatcaag gtcttttcac ctggcccgca tggacaccca
      661 gaccaggtcc cctacatcgt gacctgggaa gccttggctt ttgacccccc tccctgggtc
      721 aagccctttg tacaccctaa gcctccgcct cctcttcctc catccgcccc gtctctcccc
      781 cttgaacctc ctcgttcgac cccgcctcga tcctcccttt atccagccct cactccttct
      841 ctaggcgccc ccatatggcc atatgagatc ttatatgggg cacccccgcc ccttgtaaac
      901 ttccctgacc ctgacatgac aagagttact aacagcccct ctctccaagc tcacttacag
      961 gctctctact tagtccagca cgaagtctgg agacctctgg cggcagccta ccaagaacaa
     1021 ctggaccgac cggtggtacc tcacccttac cgagtcggcg acacagtgtg ggtccgccga
     1081 caccagacta agaacctaga acctcgctgg aaaggacctt acacagtcct gctgaccacc
     1141 cccaccgccc tcaaagtaga cggcatcgca gcttggatac acgccgccca cgtgaaggct
     1201 gccgaccccg ggggtggacc atcctctaga ctgccggatc atcgatacca tggtgagcaa
     1261 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     1321 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     1381 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     1441 cctgtcctgg ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
     1501 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     1561 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     1621 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     1681 caactacttt agcgacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc
     1741 caacttcaag atccgccaca acatcgagga cggcggcgtg cagctcgccg accactacca
     1801 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     1861 ccagtccaag ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     1921 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagggtg gcggtggctc
     1981 gggcggtggt gggtcgggtg gcggcggatc tatcgatgaa ttgaattcac gcgttcgcga
     2041 gttaacggat ccgatgtctg aagatgaaga aaaagtgaaa ttacgccgtc ttgaaccagc
     2101 tatccagaaa ttcattaaga tagtaatccc aacagacctg gaaaggttaa gaaagcacca
     2161 gataaatatt gagaagtatc aaaggtgcag aatctgggac aagttgcatg aagagcatat
     2221 caatgcagga cgtacagttc agcaactccg atccaatatc cgagaaattg agaaactttg
     2281 tttgaaagtc cgaaaggatg acctagtact tctgaagaga atgatagatc ctgttaaaga
     2341 agaagcatca gcagcaacag cagaatttct ccaactccat ttggaatctg tagaagaact
     2401 taagaagcaa tttaatgatg aagaaacttt gctacagcct cctttgacca gatccatgac
     2461 tgttggtgga gcatttcata ctactgaagc tgaagctagt tctcagagtt tgactcagat
     2521 atatgcctta cctgaaattc ctcaagatca aaatgctgca gaatcgtggg aaaccttaga
     2581 agcggactta attgaactta gccaactggt cactgacttc tctctcctag tgaattctca
     2641 gcaggagaag attgacagca ttgcagacca tgtcaacagt gctgctgtga atgttgaaga
     2701 gggaaccaaa aacttaggga aggctgcaaa atacaagctg gcagctctgc ctgtggcagg
     2761 tgcactcatc gggggaatgg tagggggtcc tattggcctc cttgcaggct tcaaagtggc
     2821 aggaattgca gctgcacttg gtggtggggt gttgggcttc acaggtggaa aattgataca
     2881 aagaaagaaa cagaaaatga tggagaagct cacttccagc tgtccagatc ttcccagcca
     2941 aactgacaag aaatgcagtt aaggatccct cgagcggccg ctacgtaaat tccgcccccc
     3001 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     3061 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     3121 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     3181 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     3241 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     3301 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     3361 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     3421 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     3481 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     3541 gaaaaacacg atgataatat ggccacaacc atgaccgagt acaagcccac ggtgcgcctc
     3601 gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac
     3661 cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac cgagctgcaa
     3721 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     3781 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     3841 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     3901 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     3961 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     4021 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     4081 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     4141 tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc
     4201 gaaaggagcg cacgacccca tggctcgacg ataaaataaa agattttatt tagtctccag
     4261 aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca
     4321 ttttgcaagg catggaaaaa tacataactg agaatagaga agttcagatc aaggtcagga
     4381 acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
     4441 ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat atctgtggta
     4501 agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg gtccagccct
     4561 cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc
     4621 tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct
     4681 ccccgagctc aataaaagag cccacaaccc ctcactcggg gcgccagtcc tccgattgac
     4741 tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc cgacttgtgg
     4801 tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag cgggggtctt
     4861 tcacatgcag catgtatcaa aattaatttg gttttttttc ttaagtattt acattaaatg
     4921 gccatagttg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     4981 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     5041 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     5101 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     5161 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     5221 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     5281 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     5341 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     5401 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     5461 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     5521 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     5581 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     5641 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     5701 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     5761 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     5821 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttgcggccgg ccgcaaatca
     5881 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     5941 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     6001 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     6061 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     6121 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     6181 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     6241 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     6301 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     6361 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     6421 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     6481 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat
     6541 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     6601 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     6661 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     6721 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     6781 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     6841 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     6901 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     6961 acgaggccct ttcgtcttca ctcgaggagc ttggcccatt gcatacgttg tatccatatc
     7021 ataatatgta catttatatt ggctcatgtc caacattacc gccatgttga cattgattat
     7081 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     7141 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     7201 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     7261 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     7321 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     7381 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     7441 ttaccatgct agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct
     7501 gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc
     7561 cccgagctca ataaaagagc ccacaacccc tcactcggcg cgccagtcct ccgattgact
     7621 gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt
     7681 ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt
     7741 catttggggg ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccacc
//
