LOCUS       sequence-179969-       13204 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transfer plasmid for the production of lentiviral vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13204)
  AUTHORS   Merienne N, Vachey G, de Longprez L, Meunier C, Zimmer V, Perriard 
            G, Canales M, Mathias A, Herrgott L, Beltraminelli T, Maulet A, 
            Dequesne T, Pythoud C, Rey M, Pellerin L, Brouillet E, Perrier AL, 
            du Pasquier R, Deglon N
  TITLE     The Self-Inactivating KamiCas9 System for the Editing of CNS Disease
            Genes.
  JOURNAL   Cell Rep. 2017 Sep 19;20(12):2980-2991. doi: 
            10.1016/j.celrep.2017.08.075.
  PUBMED    28930690
REFERENCE   2  (bases 1 to 13204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.08"; journalName: "Cell Rep"; date: 
            "2017-09-19- 19"; volume: "20"; issue: "12"; pages: "2980-2991"
COMMENT     Sequence Label: SIN-CMV-Ca9-WPRE
FEATURES             Location/Qualifiers
     source          1..13204
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(45..65)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        50..379
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      230..365
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          1513..1578
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1708..1728
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2153..2287
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2190..2209)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2244..2263
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2455..3088
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3135..3260
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3753..3986
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4171..4215
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4482..4599
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4622..4925
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4926..5129
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5080..5100
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5126..5150
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    5256..5280
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5286..9389
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5286..9389
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5286..9389
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             9402..9422
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(9466..9490)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(9513..9529)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9514..9530)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    9543..10131
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9596..9616)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10014..10025)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10220..10453
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(10478..10496)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(10479..10496)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(10786..10805)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(11019..11038)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11138..11160
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11198..11216)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11284..11388
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11389..11457,11458..12249)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11607..11626)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12420..13008
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12909..12928
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13162..13179
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctgtggaatg tgtgtcagtt
       61 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      121 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      181 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
      241 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
      301 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
      361 aggcctaggc ttttgcaaaa agcttggaca caagacaggc ttgcgagata tgtttgagaa
      421 taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa
      481 tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca
      541 ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca
      601 cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac
      661 aatggaaagg cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg
      721 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
      781 gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga
      841 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
      901 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
      961 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     1021 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
     1081 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     1141 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
     1201 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
     1261 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgcg
     1321 aaatcctcga cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
     1381 tatacgatac ggtgattggc taccgtggcg gcaactggat ttatgagtgg gccccggatc
     1441 tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt
     1501 taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta
     1561 attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat
     1621 gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct
     1681 actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag
     1741 gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt
     1801 gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg
     1861 gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt
     1921 tttcttactc cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt
     1981 acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg
     2041 actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     2101 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     2161 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     2221 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     2281 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
     2341 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
     2401 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag
     2461 ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag
     2521 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
     2581 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     2641 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     2701 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
     2761 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
     2821 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
     2881 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     2941 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     3001 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     3061 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     3121 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     3181 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     3241 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     3301 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     3361 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     3421 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     3481 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     3541 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     3601 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     3661 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     3721 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     3781 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     3841 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     3901 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     3961 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     4021 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     4081 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     4141 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     4201 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     4261 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     4321 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     4381 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     4441 tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag aaaagggggg
     4501 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     4561 aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgataagct tgggagttcc
     4621 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     4681 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     4741 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     4801 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     4861 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     4921 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     4981 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     5041 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     5101 tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac
     5161 gccatccacg ctgttttgac ctccatagaa gacaccgact ctagctagag gatcttccct
     5221 agaactagtg gatcccccgg gctgcaggaa ttatcacaag tttgtacaaa aaagcaggct
     5281 ccaccatgga caagaagtac tccattgggc tcgatatcgg cacaaacagc gtcggctggg
     5341 ccgtcattac ggacgagtac aaggtgccga gcaaaaaatt caaagttctg ggcaataccg
     5401 atcgccacag cataaagaag aacctcattg gcgccctcct gttcgactcc ggggagacgg
     5461 ccgaagccac gcggctcaaa agaacagcac ggcgcagata tacccgcaga aagaatcgga
     5521 tctgctacct gcaggagatc tttagtaatg agatggctaa ggtggatgac tctttcttcc
     5581 ataggctgga ggagtccttt ttggtggagg aggataaaaa gcacgagcgc cacccaatct
     5641 ttggcaatat cgtggacgag gtggcgtacc atgaaaagta cccaaccata tatcatctga
     5701 ggaagaagct tgtagacagt actgataagg ctgacttgcg gttgatctat ctcgcgctgg
     5761 cgcatatgat caaatttcgg ggacacttcc tcatcgaggg ggacctgaac ccagacaaca
     5821 gcgatgtcga caaactcttt atccaactgg ttcagactta caatcagctt ttcgaagaga
     5881 acccgatcaa cgcatccgga gttgacgcca aagcaatcct gagcgctagg ctgtccaaat
     5941 cccggcggct cgaaaacctc atcgcacagc tccctgggga gaagaagaac ggcctgtttg
     6001 gtaatcttat cgccctgtca ctcgggctga cccccaactt taaatctaac ttcgacctgg
     6061 ccgaagatgc caagcttcaa ctgagcaaag acacctacga tgatgatctc gacaatctgc
     6121 tggcccagat cggcgaccag tacgcagacc tttttttggc ggcaaagaac ctgtcagacg
     6181 ccattctgct gagtgatatt ctgcgagtga acacggagat caccaaagct ccgctgagcg
     6241 ctagtatgat caagcgctat gatgagcacc accaagactt gactttgctg aaggcccttg
     6301 tcagacagca actgcctgag aagtacaagg aaattttctt cgatcagtct aaaaatggct
     6361 acgccggata cattgacggc ggagcaagcc aggaggaatt ttacaaattt attaagccca
     6421 tcttggaaaa aatggacggc accgaggagc tgctggtaaa gcttaacaga gaagatctgt
     6481 tgcgcaaaca gcgcactttc gacaatggaa gcatccccca ccagattcac ctgggcgaac
     6541 tgcacgctat cctcaggcgg caagaggatt tctacccctt tttgaaagat aacagggaaa
     6601 agattgagaa aatcctcaca tttcggatac cctactatgt aggccccctc gcccggggaa
     6661 attccagatt cgcgtggatg actcgcaaat cagaagagac catcactccc tggaacttcg
     6721 aggaagtcgt ggataagggg gcctctgccc agtccttcat cgaaaggatg actaactttg
     6781 ataaaaatct gcctaacgaa aaggtgcttc ctaaacactc tctgctgtac gagtacttca
     6841 cagtttataa cgagctcacc aaggtcaaat acgtcacaga agggatgaga aagccagcat
     6901 tcctgtctgg agagcagaag aaagctatcg tggacctcct cttcaagacg aaccggaaag
     6961 ttaccgtgaa acagctcaaa gaagactatt tcaaaaagat tgaatgtttc gactctgttg
     7021 aaatcagcgg agtggaggat cgcttcaacg catccctggg aacgtatcac gatctcctga
     7081 aaatcattaa agacaaggac ttcctggaca atgaggagaa cgaggacatt cttgaggaca
     7141 ttgtcctcac ccttacgttg tttgaagata gggagatgat tgaagaacgc ttgaaaactt
     7201 acgctcatct cttcgacgac aaagtcatga aacagctcaa gaggcgccga tatacaggat
     7261 gggggcggct gtcaagaaaa ctgatcaatg ggatccgaga caagcagagt ggaaagacaa
     7321 tcctggattt tcttaagtcc gatggatttg ccaaccggaa cttcatgcag ttgatccatg
     7381 atgactctct cacctttaag gaggacatcc agaaagcaca agtttctggc cagggggaca
     7441 gtcttcacga gcacatcgct aatcttgcag gtagcccagc tatcaaaaag ggaatactgc
     7501 agaccgttaa ggtcgtggat gaactcgtca aagtaatggg aaggcataag cccgagaata
     7561 tcgttatcga gatggcccga gagaaccaaa ctacccagaa gggacagaag aacagtaggg
     7621 aaaggatgaa gaggattgaa gagggtataa aagaactggg gtcccaaatc cttaaggaac
     7681 acccagttga aaacacccag cttcagaatg agaagctcta cctgtactac ctgcagaacg
     7741 gcagggacat gtacgtggat caggaactgg acatcaatcg gctctccgac tacgacgtgg
     7801 atcatatcgt gccccagtct tttctcaaag atgattctat tgataataaa gtgttgacaa
     7861 gatccgataa aaatagaggg aagagtgata acgtcccctc agaagaagtt gtcaagaaaa
     7921 tgaaaaatta ttggcggcag ctgctgaacg ccaaactgat cacacaacgg aagttcgata
     7981 atctgactaa ggctgaacga ggtggcctgt ctgagttgga taaagccggc ttcatcaaaa
     8041 ggcagcttgt tgagacacgc cagatcacca agcacgtggc ccaaattctc gattcacgca
     8101 tgaacaccaa gtacgatgaa aatgacaaac tgattcgaga ggtgaaagtt attactctga
     8161 agtctaagct ggtctcagat ttcagaaagg actttcagtt ttataaggtg agagagatca
     8221 acaattacca ccatgcgcat gatgcctacc tgaatgcagt ggtaggcact gcacttatca
     8281 aaaaatatcc caagcttgaa tctgaatttg tttacggaga ctataaagtg tacgatgtta
     8341 ggaaaatgat cgcaaagtct gagcaggaaa taggcaaggc caccgctaag tacttctttt
     8401 acagcaatat tatgaatttt ttcaagaccg agattacact ggccaatgga gagattcgga
     8461 agcgaccact tatcgaaaca aacggagaaa caggagaaat cgtgtgggac aagggtaggg
     8521 atttcgcgac agtccggaag gtcctgtcca tgccgcaggt gaacatcgtt aaaaagaccg
     8581 aagtacagac cggaggcttc tccaaggaaa gtatcctccc gaaaaggaac agcgacaagc
     8641 tgatcgcacg caaaaaagat tgggacccca agaaatacgg cggattcgat tctcctacag
     8701 tcgcttacag tgtactggtt gtggccaaag tggagaaagg gaagtctaaa aaactcaaaa
     8761 gcgtcaagga actgctgggc atcacaatca tggagcgatc aagcttcgaa aaaaacccca
     8821 tcgactttct cgaggcgaaa ggatataaag aggtcaaaaa agacctcatc attaagcttc
     8881 ccaagtactc tctctttgag cttgaaaacg gccggaaacg aatgctcgct agtgcgggcg
     8941 agctgcagaa aggtaacgag ctggcactgc cctctaaata cgttaatttc ttgtatctgg
     9001 ccagccacta tgaaaagctc aaagggtctc ccgaagataa tgagcagaag cagctgttcg
     9061 tggaacaaca caaacactac cttgatgaga tcatcgagca aataagcgaa ttctccaaaa
     9121 gagtgatcct cgccgacgct aacctcgata aggtgctttc tgcttacaat aagcacaggg
     9181 ataagcccat cagggagcag gcagaaaaca ttatccactt gtttactctg accaacttgg
     9241 gcgcgcctgc agccttcaag tacttcgaca ccaccataga cagaaagcgg tacacctcta
     9301 caaaggaggt cctggacgcc acactgattc atcagtcaat tacggggctc tatgaaacaa
     9361 gaatcgacct ctctcagctc ggtggagaca gcagggctga ccccaagaag aagaggaagg
     9421 tgtgaaaggg ttcgatccct accggttagt aatgagttta tctagaccca gctttcttgt
     9481 acaaagtggt gataattcga tatcaagctt atcgataccg tcgacctcga gggaattccg
     9541 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     9601 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     9661 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     9721 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     9781 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     9841 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     9901 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     9961 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
    10021 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
    10081 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgggaat
    10141 tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
    10201 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
    10261 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
    10321 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
    10381 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
    10441 aaaatctcta gcagcatcta gaattaattc cgtgtattct atagtgtcac ctaaatcgta
    10501 tgtgtatgat acataaggtt atgtattaat tgtagccgcg ttctaacgac aatatgtaca
    10561 agcctaattg tgtagcatct ggcttactga agcagaccct atcatctctc tcgtaaactg
    10621 ccgtcagagt cggtttggtt ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc
    10681 cggaaatggt cagcgaacca atcagcaggg tcatcgctag ccagatcctc tacgccggac
    10741 gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca
    10801 tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg
    10861 gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat
    10921 tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg
    10981 agtcgcataa gggagagcgt cgaatggtgc actctcagta caatctgctc tgatgccgca
    11041 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
    11101 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
    11161 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
    11221 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
    11281 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
    11341 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
    11401 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
    11461 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
    11521 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
    11581 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
    11641 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
    11701 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
    11761 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
    11821 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
    11881 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
    11941 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
    12001 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
    12061 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
    12121 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
    12181 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
    12241 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
    12301 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
    12361 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
    12421 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
    12481 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
    12541 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
    12601 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    12661 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
    12721 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    12781 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
    12841 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    12901 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    12961 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    13021 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    13081 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
    13141 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
    13201 cgca
//
