LOCUS       sequence-180100-         692 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 692)
  TITLE     SGC Oxford 2017
REFERENCE   2  (bases 1 to 692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ZMYND8 (PRKCBP1A-c097)
FEATURES             Location/Qualifiers
     source          1..692
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             31..201
                     /codon_start=1
                     /product="Z domain of staphylococcal protein A, engineered 
                     to contain multiple positive charges (Hedhammar and Hober, 
                     2007)"
                     /label=Z-basic tag
                     /note="useful for purifying tagged proteins by 
                     cation-exchange chromatography"
                     /translation="VDNKFNKERRRARREIRHLPNLNREQRRAFIRSLRDDPSQSANLL
                     AEAKKLNDAQPK"
     CDS             208..228
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             672..689
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtggataaca agttcaacaa ggagcgtcga
       61 agagctcgcc gtgaaattcg ccatctgccg aacctgaacc gcgaacagcg tcgcgcattt
      121 attcgcagcc tgcgcgatga tccgagccag agcgcgaacc tgctggcgga agcgaagaag
      181 ctgaacgatg cgcagccgaa gggtaccgag aacctgtact tccaatccat gtctaaaaac
      241 actactggaa gcacaatagc tgagattcgc aggctgagga tcgagataga gaagctccag
      301 tggctgcacc agcaagagct ctccgaaatg aaacacaact tagagctgac catggcggag
      361 atgcggcaga gcctggagca ggagcgggac cggctcatcg ccgaggtgaa gaagcagctg
      421 gagttggaga agcagcaggc ggtggatgag accaagaaga agcagtggtg cgccaactgc
      481 aagaaggagg ccatctttta ctgctgttgg aacaccagct actgtgacta cccctgccag
      541 caagcccact ggcctgagca catgaagtcc tgcacccagt cagctactgc tcctcagcag
      601 gaagggtgac agtaaaggtg gatacggatc cgaattcgag ctccgtcgac aagcttgcgg
      661 ccgcactcga gcaccaccac caccaccact ga
//
