LOCUS       sequence-180177-        6820 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HCV E1E2 genes cloned from patient serum. United 
            Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone 
            number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6820)
  AUTHORS   Urbanowicz RA, McClure CP, Brown RJ, Tsoleridis T, Persson MA, Krey 
            T, Irving WL, Ball JK, Tarr AW
  TITLE     A Diverse Panel of Hepatitis C Virus Glycoproteins for Use in 
            Vaccine Research Reveals Extremes of Monoclonal Antibody 
            Neutralization Resistance.
  JOURNAL   J Virol. 2015 Dec 23;90(7):3288-301. doi: 10.1128/JVI.02700-15.
  PUBMED    26699643
REFERENCE   2  (bases 1 to 6820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-12-23- 23"; volume: "90"; issue:
            "7"; pages: "3288-301"
COMMENT     Sequence Label: UKNP3.2.3
FEATURES             Location/Qualifiers
     source          1..6820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1836..1853)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1842..2066
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2198..2786)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2278..2297)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3115..3162
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3187..3206
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3394..4182)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEHQGL
                     APAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALA
                     TRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3505..3524)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4109..4128
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4217..4574)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4231..4366
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        4383..4574
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4531..4551
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(4634..5425,5426..5494))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5257..5276
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5495..5599)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5626..6081
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5713..5732)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5923..5944
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        6093..6472
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6473..6676
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6627..6647
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6721..6740
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6721..6739
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 caccatgggt tgctctttct ctatcttcct ccttgccctg ttctcttgtt tgatccatcc
       61 agcagccagt ttagagtggc ggaatacgtc cggcctctat gtccttacca acgactgtcc
      121 caatagcagt attgtgtacg aggctgacga cgttatcctg cacacacccg gctgtatacc
      181 ctgtgttcag gacggcaaca tatctaggtg ctggacccca gtgacaccta cagtggcggt
      241 caggtacgtc ggagcaacca ccgcttcggt acgcagtcat gtggatctgt tagtgggcgc
      301 ggccacgacg tgctctgcgc tctatgtagg tgacatgtgc ggggccgtct tcctcgtggg
      361 acaagccttc acattcagac ctcgtcgcca tcaaacggtc cagacctgta actgctcact
      421 gtacccaggc catctttcag gacatcgaat ggcttgggat atgatgatga attggtcccc
      481 cgccgtgggt atggtggtag cacacgtcct gcgtctgccc cagaccttat tcgacatatt
      541 ggccggggcc cattggggca tcttggcggg tctcgcctat tattccatgc agggcaactg
      601 ggccaaggtt ggtatcatcc tggttatgtt ttccggggtc gatgccgtta cgtacatcac
      661 tggtggcact gcagctcgtg gggcccatgg gcttgctagc ttattcagtc cgggcgccag
      721 ccagcgcctg gagctgatca acaccaatgg ctcgtggcac atcaacagga ctgccctcaa
      781 ctgcaatgag tccataaaca cagggttctt agctgggttg ttttattatc ataagttcaa
      841 ctctactgga tgtccccaaa ggctcagcag ctgcaagccc atcactttct tcagacaggg
      901 gtggggcccc ttgacagatg ccaacatcac cggcccttct gatgacaaac cgtactgctg
      961 gcactacgca cctagacctt gtgacactgt taaagcagca actgtctgcg gtcctgtgta
     1021 ctgcttcaca ccatcgccag tggttgtagg cactactaat cctaagggcg ctccaaccta
     1081 taactggggc gagaatgaga cagacgtgtt cctgctgaag tccttgcggc ctcctagtgg
     1141 tcggtggttt gggtgcacgt ggatgaactc cacggggttt gtcaagacgt gcggaggtcc
     1201 cccttgtgac atctatgggg gtggggggag atccaccaat ggttcagacc tcttctgccc
     1261 caccgactgc ttcaggaaac atcctgaggc cacatacagc cggtgcggct cggggccctg
     1321 gttgacacct cgatgcatgg tcgactatcc ataccggctt tggcattacc catgtacagt
     1381 caattttaca ctgttcaagg tgaggatgtt tgtgggtggg tttgagcatc ggtttaccgc
     1441 cgcttgcaac tggactaggg gggagcgctg cgatatcgag gatcgtgacc gcagcgagca
     1501 acatcccctg ctgcattcaa caactgagct tgccatactg ccttgctctt tcacgcccat
     1561 gcccgcattg tcaacagggt taatacacct ccaccaaaac atcgtggatg tccaatacct
     1621 ttatggcgtt ggatctggca tggtgggatg ggcgttgaaa tgggaatttg tcatcctcgt
     1681 tttcctcctc ctagcagatg cacgcgtgtg cgttgccctt tggttgatgc tgatgatatc
     1741 acaggcagaa gcataattgg aagccgaatt ctgcagatat ccagcacagt ggcggccgct
     1801 cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     1861 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     1921 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     1981 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     2041 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagtg gcggtaatac
     2101 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     2161 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     2221 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2281 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     2341 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     2401 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     2461 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     2521 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     2581 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     2641 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     2701 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     2761 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     2821 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     2881 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     2941 aacctgaggc tatggcaggg cctgccgccc cgacgttggc tgcgagccct gggccttcac
     3001 ccgaacttgg ggggtggggt ggggaaaagg aagaaacgcg ggcgtattgg ccccaatggg
     3061 gtctcggtgg ggtatcgaca gagtgccagc cctgggaccg aaccccgcgt ttatgaacaa
     3121 acgacccaac accgtgcgtt ttattctgtc tttttattgc cgtcatagcg cgggttcctt
     3181 ccggtattgt ctccttccgt gtttcagtta gcctccccct agggtgggcg aagaactcca
     3241 gcatgagatc cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc
     3301 ccaacctttc atagaaggcg gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg
     3361 cttggtcggt catttcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata
     3421 gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc
     3481 ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg
     3541 gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat
     3601 gatattcggc aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgct
     3661 cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt gatcatcctg
     3721 atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg
     3781 gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat
     3841 ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc
     3901 caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac
     3961 gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct tgcagttcat tcagggcacc
     4021 ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc
     4081 ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca
     4141 agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc
     4201 tgtctcttga tcgatctttg caaaagccta ggcctccaaa aaagcctcct cactacttct
     4261 ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc
     4321 catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg
     4381 gcgggactat ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg
     4441 agcctgggga ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc
     4501 tgcctgctgg ggagcctggg gactttccac accctaactg acacacattc cacagctggt
     4561 tctttccgcc tcaggactct tcctttttca ataaatcaat ctaaagtata tatgagtaaa
     4621 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     4681 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     4741 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     4801 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     4861 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     4921 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     4981 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     5041 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     5101 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     5161 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     5221 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     5281 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     5341 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     5401 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     5461 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     5521 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     5581 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg
     5641 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     5701 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     5761 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     5821 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     5881 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     5941 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     6001 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     6061 aaatattaac gcttacaatt tacgcgcgcg ttgacattga ttattgacta gttattaata
     6121 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     6181 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     6241 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggacta
     6301 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     6361 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     6421 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     6481 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     6541 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     6601 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     6661 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
     6721 taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa gcttggtacc
     6781 gagctcggat ccactagtcc agtgtggtgg aattcggctt
//
