LOCUS       sequence-180275-        8866 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of RPS18.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8866)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 8866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: RPS18_pLX307
FEATURES             Location/Qualifiers
     source          1..8866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1777..1801)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1809..1850
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1901..2489
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1954..1974)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2372..2383)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2561..2794
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2872..2993
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2909..2928)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2963..2982
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3033..3168
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3188..3207)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3189..3207)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3217..3234)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3217..3233)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3226..3248)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3375..3830
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3462..3481)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3672..3693
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3856..3960
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3961..4029,4030..4821)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4179..4198)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4992..5580
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5481..5500
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5734..5751
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5868..5889
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5904..5909,5910..5927,5928..5934)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5942..5958
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5947..5969
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5966..5982
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5966..5982
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6001..6021
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6003..6021
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6049..6275
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6276..6456
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6503..6628
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7121..7354
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7539..7583
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7814..8024
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7836..8032
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7883..8018
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8064..8666
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8064..8083)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8560..8580
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8709..8826
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tgtctctagt gatccctgaa aagttccagc atattttgcg agtactcaac accaacatcg
     1381 atgggcggcg gaaaatagcc tttgccatca ctgccattaa gggtgtgggc cgaagatatg
     1441 ctcatgtggt gttgaggaaa gcagacattg acctcaccaa gagggcggga gaactcactg
     1501 aggatgaggt ggaacgtgtg atcaccatta tgcagaatcc acgccagtac aagatcccag
     1561 actggttctt gaacagacag aaggatgtaa aggatggaaa atacagccag gtcctagcca
     1621 atggtctgga caacaagctc cgtgaagacc tggagcgact gaagaagatt cgggcccata
     1681 gagggctgcg tcacttctgg ggccttcgtg tccgaggcca gcacaccaag accactggcc
     1741 gccgtggccg caccgtgggt gtgtccaaga agaaattgcc aactttcttg tacaaagtgg
     1801 ttgatatcgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgaa
     1861 ctagtaccgg ttaagtcgac aatcaacgcg ttaagtcgac aatcaacctc tggattacaa
     1921 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     1981 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     2041 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     2101 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     2161 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     2221 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     2281 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     2341 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     2401 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     2461 tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca
     2521 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     2581 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     2641 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     2701 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     2761 agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt
     2821 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     2881 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     2941 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     3001 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     3061 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     3121 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc
     3181 caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg
     3241 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     3301 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     3361 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     3421 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     3481 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     3541 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     3601 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     3661 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     3721 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     3781 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
     3841 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3901 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     3961 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4021 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4081 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4141 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     4201 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     4261 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     4321 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     4381 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     4441 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     4501 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     4561 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     4621 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     4681 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     4741 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     4801 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     4861 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     4921 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     4981 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5041 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5101 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     5161 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5221 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5281 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5341 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5401 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5461 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     5521 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     5581 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     5641 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     5701 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     5761 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     5821 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     5881 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     5941 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
     6001 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
     6061 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
     6121 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
     6181 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
     6241 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
     6301 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     6361 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     6421 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     6481 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     6541 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     6601 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     6661 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     6721 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     6781 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     6841 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     6901 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     6961 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     7021 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     7081 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     7141 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     7201 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     7261 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     7321 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     7381 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     7441 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     7501 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     7561 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     7621 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     7681 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     7741 caaccccgag gggacccatg cattgcatct caattagtca gcaaccaggt gtggaaagtc
     7801 cccaggctcc ccagcaggca gaagtatgca aagcatgcgt ctcaattagt cagcaaccat
     7861 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     7921 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     7981 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctttc
     8041 tagaggtacc accggagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     8101 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     8161 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     8221 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgccg
     8281 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     8341 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     8401 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     8461 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     8521 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     8581 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     8641 tgacccgcaa gcccggtgcc agttaacgaa ttctagatct tgagacaaat ggcagtattc
     8701 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     8761 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     8821 aattttcggg tttattacag ggacagcaga gatccacttt ggcgcc
//
