LOCUS       sequence-180289-       11557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ZNF217.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11557)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 11557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: ZNF217_pLX307
FEATURES             Location/Qualifiers
     source          1..11557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4468..4492)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4500..4541
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4592..5180
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4645..4665)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5063..5074)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5252..5485
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5563..5684
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5600..5619)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5654..5673
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5724..5859
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5879..5898)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5880..5898)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5908..5925)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5908..5924)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5917..5939)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6066..6521
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6153..6172)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6363..6384
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6547..6651
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6652..6720,6721..7512)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6870..6889)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7683..8271
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8172..8191
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8425..8442
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8559..8580
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8595..8600,8601..8618,8619..8625)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8633..8649
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8638..8660
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8657..8673
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8657..8673
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8692..8712
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8694..8712
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8740..8966
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8967..9147
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9194..9319
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9812..10045
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10230..10274
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10505..10715
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        10527..10723
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10574..10709
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10755..11357
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10755..10774)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11251..11271
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11400..11517
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tgcaatcgaa agtgacagga aacatgccaa ctcaatccct cttaatgtac atggatgggc
     1381 cagaagtgat tggcagctct cttggcagtc cgatggagat ggaggatgcc ttgtcaatga
     1441 aagggaccgc tgttgttcca ttccgagcta cacaagaaaa aaatgtcatc caaatcgagg
     1501 ggtatatgcc cttggattgc atgttctgca gccagacctt cacacattca gaagacctta
     1561 ataaacatgt cttaatgcaa caccggccta ccctctgtga accagcagtt cttcgggttg
     1621 aagcagagta tctcagtccg cttgataaaa gtcaagtgcg aacagaacct cccaaggaaa
     1681 agaattgcaa ggaaaatgaa tttagctgtg aggtatgtgg gcagacattt agagtcgctt
     1741 ttgatgttga gatccacatg agaacacaca aagattcttt cacttacggg tgtaacatgt
     1801 gcggaagaag attcaaggag ccttggtttc ttaaaaatca catgcggaca cataatggca
     1861 aatcgggggc cagaagcaaa ctgcagcaag gcttggagag tagtccagca acgatcaacg
     1921 aggtcgtcca ggtgcacgcg gccgagagca tctcctctcc ttacaaaatc tgcatggttt
     1981 gtggcttcct atttccaaat aaagaaagtc taattgagca ccgcaaggtg cacaccaaaa
     2041 aaactgcttt cggtaccagc agcgcgcaga cagactctcc acaaggagga atgccgtcct
     2101 cgagggagga cttcctgcag ttgttcaact tgagaccaaa atctcaccct gaaacgggga
     2161 agaagcctgt cagatgcatc cctcagctcg atccgttcac caccttccag gcttggcagc
     2221 tggctaccaa aggaaaagtt gccatttgcc aagaagtgaa ggaatcgggg caagaaggga
     2281 gcaccgacaa cgacgattcg agttccgaga aggagcttgg agaaacaaat aagggcagtt
     2341 gtgcaggcct ctcgcaagag aaagagaagt gcaaacactc ccacggcgaa gcgccctccg
     2401 tggacgcgga tcccaagtta cccagtagca aggagaagcc cactcactgc tccgagtgcg
     2461 gcaaagcttt cagaacctac caccagctgg tcttgcactc cagggtccac aagaaggacc
     2521 ggagggccgg cgcggagtcg cccaccatgt ctgtggacgg gaggcagccg gggacgtgtt
     2581 ctcctgacct cgccgcccct ctggatgaaa atggagccgt ggatcgaggg gaaggtggtt
     2641 ctgaagacgg atctgaggat gggcttcccg aaggaatcca tctggataaa aatgatgatg
     2701 gaggaaaaat aaaacatctt acatcttcaa gagagtgtag ttattgtgga aagtttttcc
     2761 gttcaaatta ttacctcaat attcatctca gaacgcatac aggtgaaaaa ccatacaaat
     2821 gtgaattttg tgaatatgct gcagcccaga agacatctct gaggtatcac ttggagagac
     2881 atcacaagga aaaacaaacc gatgttgctg ctgaagtcaa gaacgatggt aaaaatcagg
     2941 acactgaaga tgcactatta accgctgaca gtgcgcaaac caaaaatttg aaaagatttt
     3001 ttgatggtgc caaagatgtt acaggcagtc cacctgcaaa gcagcttaag gagatgcctt
     3061 ctgtttttca gaatgttctg ggcagcgctg tcctctcacc agcacacaaa gatactcagg
     3121 atttccataa aaatgcagct gatgacagtg ctgataaagt gaataaaaac cctacccctg
     3181 cttacctgga cctgttaaaa aagagatcag cagttgaaac tcaggcaaat aacctcatct
     3241 gtagaaccaa ggcggatgtt actcctcctc cggatggcag taccacccat aaccttgaag
     3301 ttagccccaa agagaagcaa acggagaccg cagctgactg cagatacagg ccaagtgtgg
     3361 attgtcacga aaaaccttta aatttatccg tgggggctct tcacaattgc ccggcaattt
     3421 ctttgagtaa aagtttgatt ccaagtatca cctgtccatt ttgtaccttc aagacatttt
     3481 atccagaagt tttaatgatg caccagagac tggagcataa atacaatcct gacgttcata
     3541 aaaactgtcg aaacaagtcc ttgcttagaa gtcgacgtac cggatgcccg ccagcgttgc
     3601 tgggaaaaga tgtgcctccc ctctctagtt tctgtaaacc caagcccaag tctgctttcc
     3661 cggcgcagtc caaatccctg ccatctgcga aggggaagca gagccctcct gggccaggca
     3721 aggcccctct gacttcaggg atagactcta gcactttagc cccaagtaac ctgaagtccc
     3781 acagaccaca gcagaatgtg ggggtccaag gggccgccac caggcaacag caatctgaga
     3841 tgtttcctaa aaccagtgtt tcccctgcac cggataagac aaaaagaccc gagacaaaat
     3901 tgaaacctct tccagtagct ccttctcagc ccaccctcgg cagcagtaac atcaatggtt
     3961 ccatcgacta ccccgccaag aacgacagcc cgtgggcacc tccgggaaga gactatttct
     4021 gtaatcggag tgccagcaat actgcagcag aatttggtga gccccttcca aaaagactga
     4081 agtccagcgt ggttgccctt gacgttgacc agcccggggc caattacaga agaggctatg
     4141 accttcccaa gtaccatatg gtcagaggca tcacatcact gttaccgcag gactgtgtgt
     4201 atccgtcgca ggcgctgcct cccaaaccaa ggttcctgag ctccagcgag gtcgattctc
     4261 caaatgtgct gactgttcag aagccctatg gtggctccgg gccactttac acttgtgtgc
     4321 ctgctggtag tccagcatcc agctcgacgt tagaaggaaa aaggcctgtg tcatatcaac
     4381 acttatctaa cagcatggca caaaagagaa actatgagaa ttttattggg aatgcacatt
     4441 atcgaccaaa tgacaaaaaa actccacccc caactttctt gtacaaagtg gttgatatcg
     4501 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga actagtaccg
     4561 gttaagtcga caatcaacgc gttaagtcga caatcaacct ctggattaca aaatttgtga
     4621 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     4681 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     4741 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     4801 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     4861 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     4921 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     4981 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     5041 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     5101 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     5161 cctttgggcc gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat
     5221 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     5281 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     5341 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     5401 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     5461 tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag
     5521 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     5581 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     5641 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     5701 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     5761 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     5821 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc
     5881 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     5941 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     6001 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     6061 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     6121 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     6181 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     6241 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     6301 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     6361 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     6421 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     6481 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     6541 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     6601 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     6661 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     6721 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     6781 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     6841 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     6901 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     6961 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     7021 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     7081 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     7141 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     7201 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     7261 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     7321 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     7381 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     7441 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     7501 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     7561 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     7621 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     7681 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     7741 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     7801 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     7861 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     7921 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     7981 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     8041 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     8101 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     8161 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     8221 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     8281 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     8341 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     8401 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     8461 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     8521 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     8581 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     8641 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
     8701 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
     8761 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
     8821 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
     8881 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
     8941 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
     9001 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     9061 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     9121 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     9181 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     9241 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     9301 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     9361 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     9421 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     9481 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     9541 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     9601 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     9661 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     9721 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     9781 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     9841 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     9901 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     9961 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
    10021 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
    10081 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
    10141 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
    10201 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
    10261 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
    10321 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
    10381 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
    10441 ggggacccat gcattgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
    10501 cccagcaggc agaagtatgc aaagcatgcg tctcaattag tcagcaacca tagtcccgcc
    10561 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
    10621 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
    10681 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttt ctagaggtac
    10741 caccggagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
    10801 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
    10861 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
    10921 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcc gtggcggtct
    10981 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
    11041 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
    11101 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
    11161 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
    11221 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
    11281 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
    11341 agcccggtgc cagttaacga attctagatc ttgagacaaa tggcagtatt catccacaat
    11401 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
    11461 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
    11521 gtttattaca gggacagcag agatccactt tggcgcc
//
