LOCUS       sequence-180294-        6813 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HCV E1E2 genes cloned from patient serum. United 
            Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone 
            number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6813)
  AUTHORS   Urbanowicz RA, McClure CP, Brown RJ, Tsoleridis T, Persson MA, Krey 
            T, Irving WL, Ball JK, Tarr AW
  TITLE     A Diverse Panel of Hepatitis C Virus Glycoproteins for Use in 
            Vaccine Research Reveals Extremes of Monoclonal Antibody 
            Neutralization Resistance.
  JOURNAL   J Virol. 2015 Dec 23;90(7):3288-301. doi: 10.1128/JVI.02700-15.
  PUBMED    26699643
REFERENCE   2  (bases 1 to 6813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-12-23- 23"; volume: "90"; issue:
            "7"; pages: "3288-301"
COMMENT     Sequence Label: UKNP6.1.2
FEATURES             Location/Qualifiers
     source          1..6813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1829..1846)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1835..2059
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2191..2779)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2271..2290)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3108..3155
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3180..3199
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3387..4175)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEHQGL
                     APAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALA
                     TRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3498..3517)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4102..4121
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4210..4567)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4224..4359
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        4376..4567
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4524..4544
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(4627..5418,5419..5487))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5250..5269
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5488..5592)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5619..6074
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5706..5725)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5916..5937
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        6086..6465
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6466..6669
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6620..6640
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6714..6733
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6714..6732
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 caccatgggt tgctctttct ctatcttcct tttggcacta ctctcgtgcc tcacaacgcc
       61 ggcttcggct cttacctacg gtaactccag tgggctgtac catcttacaa atgattgccc
      121 caactccagc atcgtgctgg aagcggatgc tatgatcttg catctgcctg gatgcttgcc
      181 ttgtgtgagg gtcgataatc ggtccacctg ctggcatgct gtgtccccca ccctagccat
      241 accaaatgct tctacgcccg caacgggatt ccgcaggcat gtggatcttc ttgcgggcgc
      301 cgcagtggtc tgctcatccc tatacattgg ggatctgtgc ggctctctct ttttggcggg
      361 acaactattc accttccagc cccgccgaca ttggaccgtg caagactgca actgctccat
      421 ctatacaggc cacgttaccg gccacaggat ggcttgggac atgatgatga attggtcacc
      481 cacaaccact ctggtcctat ctagcatctt gagggtacct gagatctgtg cgagtgtgat
      541 actcggtggc cactggggga tactattggc cgtcgcctac tttggtatgg ctggcaactg
      601 gctaaaagtt ctggctgttc tgttcttatt tgcaggggtt gaagcaacca ccaccaccgc
      661 ccacgaagcg gcccgcgcta ctgccggctt cgttgggctt ttcacctccg gtgccaggca
      721 gaagctacaa ctcatcaaca ccaacggcag ctggcatata aacaggactg ctttgaactg
      781 caatgattcc ctccagacgg ggttcatagc gtcactcttt tacttcaaca aattcaactc
      841 ctcgggttgc ccggagcgga tggctgcgtg taagcccctc gcggatttcc ggcaggggtg
      901 gggccaaata acctacaagg tcaacatctc gggcccctct gacgaccgtc cctactgctg
      961 gcattacgct cctaggtcat gtgacgtggt gccggcccgc acggtgtgcg gccctgttta
     1021 ctgcttcacg cccagccctg ttgtggtagg aaccactgat aggcggggca accccacata
     1081 cacctggggg gagaatgaga cggacgtatt catgctggaa agccttcggc cccctactgg
     1141 aggatggttt gggtgcacgt ggatgaactc tacgggcttt accaagacct gtggtgcccc
     1201 gccatgtcag atagtcccag gagattacaa cagctctgcc aacgagcttt tgtgccccac
     1261 cgactgcttc cgtaaacatc cggaagctac ataccagcgg tgcggatcgg gaccctggct
     1321 gacacctagg tgtctggtgg attaccctta caggctatgg cactacccct gcactgtcaa
     1381 ctttaccgtg cataaagtca ggatgttcgt gggaggcatt gagcaccggt ttgacgctgc
     1441 atgtaattgg accaggggcg agcggtgcga gctacatgac agagacagga ttgaaatgag
     1501 cccgctgctt ttctcaacta cgcagctcgc catacttccc tgctcatttt ccaccatgcc
     1561 ggccttgtca actggcctca tccatctgca tcagaacata gtggacgtgc agtacctcta
     1621 cggagtatcc tcgagcgtca cctcgtgggt ggtgaagtgg gagtacattg tcctgatgtt
     1681 cctggttctg gcagatgctc ggatttgtac gtgtctctgg ttaatgctgc tcataaccaa
     1741 cgttgaagca taaaagccga attctgcaga tatccagcac agtggcggcc gctcgagtct
     1801 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     1861 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     1921 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     1981 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     2041 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gtggcggtaa tacggttatc
     2101 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     2161 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     2221 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     2281 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2341 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     2401 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2461 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2521 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     2581 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     2641 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     2701 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2761 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     2821 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     2881 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaacctga
     2941 ggctatggca gggcctgccg ccccgacgtt ggctgcgagc cctgggcctt cacccgaact
     3001 tggggggtgg ggtggggaaa aggaagaaac gcgggcgtat tggccccaat ggggtctcgg
     3061 tggggtatcg acagagtgcc agccctggga ccgaaccccg cgtttatgaa caaacgaccc
     3121 aacaccgtgc gttttattct gtctttttat tgccgtcata gcgcgggttc cttccggtat
     3181 tgtctccttc cgtgtttcag ttagcctccc cctagggtgg gcgaagaact ccagcatgag
     3241 atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct
     3301 ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc
     3361 ggtcatttcg aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg
     3421 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg
     3481 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc
     3541 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc
     3601 ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gctcgccttg
     3661 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttgatcatc ctgatcgaca
     3721 agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat
     3781 gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact
     3841 ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc
     3901 agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc
     3961 gtggccagcc acgatagccg cgctgcctcg tcttgcagtt cattcagggc accggacagg
     4021 tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca
     4081 gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc
     4141 ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct
     4201 tgatcgatct ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag
     4261 ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg
     4321 cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac
     4381 tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     4441 ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     4501 tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagct ggttctttcc
     4561 gcctcaggac tcttcctttt tcaataaatc aatctaaagt atatatgagt aaacttggtc
     4621 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     4681 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     4741 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     4801 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     4861 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     4921 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     4981 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     5041 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     5101 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     5161 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     5221 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     5281 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     5341 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     5401 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     5461 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     5521 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     5581 aggggttccg cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt
     5641 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     5701 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     5761 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     5821 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     5881 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     5941 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     6001 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     6061 aacgcttaca atttacgcgc gcgttgacat tgattattga ctagttatta atagtaatca
     6121 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     6181 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     6241 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga ctatttacgg
     6301 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     6361 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     6421 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     6481 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     6541 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     6601 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     6661 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
     6721 actcactata gggagaccca agctggctag cgtttaaact taagcttggt accgagctcg
     6781 gatccactag tccagtgtgg tggaattcgg ctt
//
