LOCUS       sequence-180574-        9333 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses 7A mutant BRD4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9333)
  AUTHORS   Shu S, Lin CY, He HH, Witwicki RM, Tabassum DP, Roberts JM, 
            Janiszewska M, Huh SJ, Liang Y, Ryan J, Doherty E, Mohammed H, Guo 
            H, Stover DG, Ekram MB, Peluffo G, Brown J, D'Santos C, Krop IE, 
            Dillon D, McKeown M, Ott C, Qi J, Ni M, Rao PK, Duarte M, Wu SY, 
            Chiang CM, Anders L, Young RA, Winer EP, Letai A, Barry WT, Carroll 
            JS, Long HW, Brown M, Liu XS, Meyer CA, Bradner JE, Polyak K
  TITLE     Response and resistance to BET bromodomain inhibitors in 
            triple-negative breast cancer.
  JOURNAL   Nature. 2016 Jan 21;529(7586):413-7. doi: 10.1038/nature16508. Epub 
            2016 Jan 6.
  PUBMED    26735014
REFERENCE   2  (bases 1 to 9333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature16508"; journalName: "Nature"; date: "2016-01-21- 
            21"; volume: "529"; issue: "7586"; pages: "413-7"
COMMENT     Sequence Label: pcDNA5-Flag-BRD4-7A
FEATURES             Location/Qualifiers
     source          1..9333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        103..482
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        483..686
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     637..657
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    688..706
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    709..727
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     737..761
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             860..883
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3101..3118
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5153..5170)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5159..5383
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5516..5535)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5667..5714
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5722..6744
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5722..6744
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6874..6995
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6911..6930)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6965..6984
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7044..7060)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7044..7060)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7057..7079)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7068..7084
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7092..7098,7099..7116,7117..7122))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7137..7158
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7275..7292)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7446..8034)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7526..7545)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8205..8996,8997..9065))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8828..8847
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9066..9170)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9245..9264)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
       61 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta
      121 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      181 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      241 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      301 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      361 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      421 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      481 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      541 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      601 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
      661 ggtgggaggt ctatataagc agagctctcc ctatcagtga tagagatctc cctatcagtg
      721 atagagatcg tcgacgagct cgtttagtga accgtcagat cgcctggaga cgccatccac
      781 gctgttttga cctccataga agacaccggg accgatccag cctccggact ctagcgttta
      841 aacttaagct tccaccatgg attacaagga tgacgacgat aagggatcta tgtctgcgga
      901 gagcggccct gggacgagat tgagaaatct gccagtaatg ggggatggac tagaaacttc
      961 ccaaatgtct acaacacagg cccaggccca accccagcca gccaacgcag ccagcaccaa
     1021 ccccccgccc ccagagacct ccaaccctaa caagcccaag aggcagacca accaactgca
     1081 atacctgctc agagtggtgc tcaagacact atggaaacac cagtttgcat ggcctttcca
     1141 gcagcctgtg gatgccgtca agctgaacct ccctgattac tataagatca ttaaaacgcc
     1201 tatggatatg ggaacaataa agaagcgctt ggaaaacaac tattactgga atgctcagga
     1261 atgtatccag gacttcaaca ctatgtttac aaattgttac atctacaaca agcctggaga
     1321 tgacatagtc ttaatggcag aagctctgga aaagctcttc ttgcaaaaaa taaatgagct
     1381 acccacagaa gaaaccgaga tcatgatagt ccaggcaaaa ggaagaggac gtgggaggaa
     1441 agaaacaggg acagcaaaac ctggcgtttc cacggtacca aacacaactc aagcatcgac
     1501 tcctccgcag acccagaccc ctcagccgaa tcctcctcct gtgcaggcca cgcctcaccc
     1561 cttccctgcc gtcaccccgg acctcatcgt ccagacccct gtcatgacag tggtgcctcc
     1621 ccagccactg cagacgcccc cgccagtgcc cccccagcca caacccccac ccgctccagc
     1681 tccccagccc gtacagagcc acccacccat catcgcggcc accccacagc ctgtgaagac
     1741 aaagaaggga gtgaagagga aagcagacac caccaccccc accaccattg accccattca
     1801 cgagccaccc tcgctgcccc cggagcccaa gaccaccaag ctgggccagc ggcgggagag
     1861 cagccggcct gtgaaacctc caaagaagga cgtgcccgac tctcagcagc acccagcacc
     1921 agagaagagc agcaaggtct cggagcagct caagtgctgc agcggcatcc tcaaggagat
     1981 gtttgccaag aagcacgccg cctacgcctg gcccttctac aagcctgtgg acgtggaggc
     2041 actgggccta cacgactact gtgacatcat caagcacccc atggacatga gcacaatcaa
     2101 gtctaaactg gaggcccgtg agtaccgtga tgctcaggag tttggtgctg acgtccgatt
     2161 gatgttctcc aactgctata agtacaaccc tcctgaccat gaggtggtgg ccatggcccg
     2221 caagctccag gatgtgttcg aaatgcgctt tgccaagatg ccggacgagc ctgaggagcc
     2281 agtggtggcc gtgtcctccc cggcagtgcc ccctcccacc aaggttgtgg ccccgcccgc
     2341 atccagcgac gccagcagcg atgcctccgc ggacagtgac gctgcgactg atgacgctga
     2401 ggaggagcga gcccagcggc tggctgagct ccaggagcag ctcaaagccg tgcacgagca
     2461 gcttgcagcc ctctctcagc cccagcagaa caaaccaaag aaaaaggaga aagacaagaa
     2521 ggaaaagaaa aaagaaaagc acaaaaggaa agaggaagtg gaagagaata aaaaaagcaa
     2581 agccaaggaa cctcctccta aaaagacgaa gaaaaataat agcagcaaca gcaatgtgag
     2641 caagaaggag ccagcgccca tgaagagcaa gccccctccc acgtatgagt cggaggaaga
     2701 ggacaagtgc aagcctatgt cctatgagga gaagcggcag ctcagcttgg acatcaacaa
     2761 gctccccggc gagaagctgg gccgcgtggt gcacatcatc cagtcacggg agccctccct
     2821 gaagaattcc aaccccgacg agattgaaat cgactttgag accctgaagc cgtccacact
     2881 gcgtgagctg gagcgctatg tcacctcctg tttgcggaag aaaaggaaac ctcaagctga
     2941 gaaagttgat gtgattgccg gctcctccaa gatgaagggc ttctcgtcct cagagtcgga
     3001 gagctccagt gagtccagct cctctgacag cgaagactcc gaaacagaga tggctccgaa
     3061 gtcaaaaaag aaggggcacc ccgggaggga gcagaagaag caccatcatc accaccatca
     3121 gcagatgcag caggccccgg ctcctgtgcc ccagcagccg cccccgcctc cccagcagcc
     3181 cccaccgcct ccacctccgc agcagcaaca gcagccgcca cccccgcctc ccccaccctc
     3241 catgccgcag caggcagccc cggcgatgaa gtcctcgccc ccacccttca ttgccaccca
     3301 ggtgcccgtc ctggagcccc agctcccagg cagcgtcttt gaccccatcg gccacttcac
     3361 ccagcccatc ctgcacctgc cgcagcctga gctgccccct cacctgcccc agccgcctga
     3421 gcacagcact ccaccccatc tcaaccagca cgcagtggtc tctcctccag ctttgcacaa
     3481 cgcactaccc cagcagccat cacggcccag caaccgagcc gctgccctgc ctcccaagcc
     3541 cgcccggccc ccagccgtgt caccagcctt gacccaaaca cccctgctcc cacagccccc
     3601 catggcccaa cccccccaag tgctgctgga ggatgaagag ccacctgccc cacccctcac
     3661 ctccatgcag atgcagctgt acctgcagca gctgcagaag gtgcagcccc ctacgccgct
     3721 actcccttcc gtgaaggtgc agtcccagcc cccacccccc ctgccgcccc caccccaccc
     3781 ctctgtgcag cagcagctgc agcagcagcc gccaccaccc ccaccacccc agccccagcc
     3841 tccaccccag cagcagcatc agccccctcc acggcccgtg cacttgcagc ccatgcagtt
     3901 ttccacccac atccaacagc ccccgccacc ccagggccag cagccccccc atccgccccc
     3961 aggccagcag ccacccccgc cgcagcctgc caagcctcag caagtcatcc agcaccacca
     4021 ttcaccccgg caccacaagt cggaccccta ctcaaccggt cacctccgcg aagccccctc
     4081 cccgcttatg atacattccc cccagatgtc acagttccag agcctgaccc accagtctcc
     4141 accccagcaa aacgtccagc ctaagaaaca ggagctgcgt gctgcctccg tggtccagcc
     4201 ccagcccctc gtggtggtga aggaggagaa gatccactca cccatcatcc gcagcgagcc
     4261 cttcagcccc tcgctgcggc cggagccccc caagcacccg gagagcatca aggcccccgt
     4321 ccacctgccc cagcggccgg aaatgaagcc tgtggatgtc gggaggcctg tgatccggcc
     4381 cccagagcag aacgcaccgc caccaggggc ccctgacaag gacaaacaga aacaggagcc
     4441 gaagactcca gttgcgccca aaaaggacct gaaaatcaag aacatgggct cctgggccag
     4501 cctagtgcag aagcatccga ccaccccctc ctccacagcc aagtcatcca gcgacagctt
     4561 cgagcagttc cgccgcgccg ctcgggagaa agaggagcgt gagaaggccc tgaaggctca
     4621 ggccgagcac gctgagaagg agaaggagcg gctgcggcag gagcgcatga ggagccgaga
     4681 ggacgaggat gcgctggagc aggcccggcg ggcccatgag gaggcacgtc ggcgccagga
     4741 gcagcagcag cagcagcgcc aggagcaaca gcagcagcag caacagcaag cagctgcggt
     4801 ggctgccgcc gccaccccac aggcccagag ctcccagccc cagtccatgc tggaccagca
     4861 gagggagttg gcccggaagc gggagcagga gcgaagacgc cgggaagcca tggcagctac
     4921 cattgacatg aatttccaga gtgatctatt gtcaatattt gaagaaaatc ttttctgagc
     4981 gcacctaggt ggcttctgac tttgattttc tggcaaaaca ttgactttcc atagtgttag
     5041 gggcggtggt ggaggtggga tcagcggcca ggggatgcct cagggcctgg ccctcctgca
     5101 tgcaagcttc agctgctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact
     5161 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     5221 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     5281 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     5341 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     5401 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     5461 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     5521 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     5581 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5641 gattagggtg atggttcacg tacctagaag ttcctattcc gaagttccta ttctctagaa
     5701 agtataggaa cttccttggc caaaaagcct gaactcaccg cgacgtctgt cgagaagttt
     5761 ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct
     5821 cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc
     5881 gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt
     5941 ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt
     6001 gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg
     6061 gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc
     6121 ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt
     6181 gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc
     6241 gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc
     6301 gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc
     6361 attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc
     6421 tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg
     6481 gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc
     6541 tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac
     6601 gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg
     6661 gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc
     6721 actcgtccga gggcaaagga atagcacgta ctacgagatt tcgattccac cgccgccttc
     6781 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     6841 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     6901 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6961 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     7021 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     7081 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     7141 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7201 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7261 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7321 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     7381 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7441 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7501 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7561 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7621 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     7681 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     7741 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     7801 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     7861 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     7921 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     7981 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     8041 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     8101 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     8161 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     8221 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     8281 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     8341 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     8401 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     8461 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     8521 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     8581 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     8641 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     8701 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     8761 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     8821 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     8881 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     8941 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     9001 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     9061 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     9121 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     9181 cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc
     9241 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
     9301 tggaggtcgc tgagtagtgc gcgagcaaaa ttt
//
