LOCUS       sequence-180957-        8945 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8945)
  AUTHORS   Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA
  TITLE     Human Induced Pluripotent Stem Cells Free of Vector and Transgene 
            Sequences
  JOURNAL   Science. 2009 Mar 26.
  PUBMED    19325077
REFERENCE   2  (bases 1 to 8945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2009 Mar 26."
COMMENT     Sequence Label: pSIN4-CMV-K2M
FEATURES             Location/Qualifiers
     source          1..8945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(863..1066)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(892..912)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1067..1370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    1462..1579
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1866..1910)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2095..2328
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2821..2946
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2993..3626
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(3785..4373)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3865..3884)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4544..5335,5336..5404))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5167..5186
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5405..5509)
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      5553..5562
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5605..5829
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     5818..5835
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             5986..6219
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(6328..7647)
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     misc_feature    7707..8259
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7709..8282
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7869..7888)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     8098..8115
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 gcggcggccc gccgttgtag ggcgccacca ccaccgggtg gctgccgtca gggctgcctt
       61 tgctgacgct gatgaccgac gggctgccgt actcgctgcc aggggcgctc agcgacgcct
      121 tcagcacgaa cttgcccatc agcccgccac ctggcggctg cggctgctgc ggcggaatgt
      181 acaccgggtc caattctggc cgcaggagct cggccacgaa gccgcccgag gggctcacgt
      241 cgttgatgtc cgccaggttg aagggagccg tcggaggggg agcggactcc ctgccataga
      301 ggaggcctcc gcccgtgccg cccggcgcca cgcccgggtc gttcccggcc cggatcggat
      361 aggtgaagct gcaggtggag ggcgcgctgg cagggccgct gctcgacggc gacgacgaag
      421 aggaggctga cgctgacgag gacacggtgg cggccactga ctccggagga tgggtcagcg
      481 aattggagag aataaagtcc aggtccagga gatcgttgaa ctcctcggtc tctctccgag
      541 gtaggggcgc caggttgcta ccgccgcaag ccgcaccggc tccgccgctc tccaggtctg
      601 tggccacggt cgccgccgcc aggtcatagg ggcggccggg aagcactggg ggaagtcgct
      661 tcatgtggga gagctcctcc cgccagcggt tattcggggc acctgcttga cgcagtgtct
      721 tctcccttcc cgccgggcca gacgcgaacg tggagaaaga tgggagcagc gcgtcgctga
      781 cagccatggc gatcgcgaat tcgaagcttg agctcgagat ctgagtccgg tagcgctagc
      841 ggatctgacg gttcactaaa ccagctctgc ttatatagac ctcccaccgt acacgcctac
      901 cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt
      961 ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt
     1021 caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga
     1081 tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat
     1141 aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat
     1201 acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat
     1261 ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg
     1321 ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca
     1381 tatatgggct atgaactaat gaccccgtaa ttgattacta ttaatccaaa gtggatctct
     1441 gctgtccctg taataaaccc gaaaattttg aatttttgta atttgttttt gtaattcttt
     1501 agtttgtatg tctgttgcta ttatgtctac tattctttcc cctgcactgt accccccaat
     1561 cccccccttt cttttaaaat tgtggatgaa tactgccatt tgtggcgata ccgtcgagat
     1621 ccgttcacta atcgaatgga tctgtctctg tctctctctc caccttcttc ttctattcct
     1681 tcgggcctgt cgggtcccct cggggttggg aggtgggtct gaaacgataa tggtgaatat
     1741 ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa acctaccaag
     1801 cctcctacta tcattatgaa taattttata taccacagcc aatttgttat gttaaaccaa
     1861 ttccacaaac ttgcccattt atctaattcc aataattctt gttcattctt ttcttgctgg
     1921 ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc
     1981 cactccatcc aggtcgtgtg attccaaatc tgttccagag atttattact ccaactagca
     2041 ttccaaggca cagcagtggt gcaaatgagt tttccagagc aaccccaaat ccccaggagc
     2101 tgttgatcct ttaggtatct ttccacagcc aggattcttg cctggagctg cttgatgccc
     2161 cagactgtga gttgcaacag atgctgttgc gcctcaatag ccctcagcaa attgttctgc
     2221 tgctgcacta taccagacaa taattgtctg gcctgtaccg tcagcgtcat tgacgctgcg
     2281 cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca aagctcctat tcccactgct
     2341 cttttttctc tctgcaccac tcttctcttt gccttggtgg gtgctactcc taatggttca
     2401 atttttacta ctttatattt atataattca cttctccaat tgtccctcat atctcctcct
     2461 ccaggtctga agatcagcgg ccgcttgctg tgcggtggtc ttacttttgt tttgctcttc
     2521 ctctatcttg tctaaagctt ccttggtgtc ttttatctct atcctttgat gcacacaata
     2581 gagggttgct actgtattat ataatgatct aagttcttct gatcctgtct gaagggatgg
     2641 ttgtagctgt cccagtattt gtctacagcc ttctgatgtt tctaacaggc caggattaac
     2701 tgcgaatcgt tctagctccc tgcttgccca tactatatgt tttaatttat attttttctt
     2761 tccccctggc cttaaccgaa ttttttccca tcgcgatcta attctccccc gcttaatact
     2821 gacgctctcg cacccatctc tctccttcta gcctccgcta gtcaaaattt ttggcgtact
     2881 caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag tcctgcgtcg
     2941 agagagctcc tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc actgctagag
     3001 attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga
     3061 cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc
     3121 cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga cccagtacag
     3181 gcaaaaagca gctgcttata tgcaggatct gagggctcgc cactccccag tcccgcccag
     3241 gccacgcctc cctggaaagt ccccagcgga aagtcccttg tagcaagctc gatatcagca
     3301 gttcttgaag tactccggat gcagctctcg ggccacgtga tgaaatgcta ggcggctgtc
     3361 aaacctccac tctaacactt ctctctccgg gtcatccatc ccatgcaggc tcacagggtg
     3421 taacaagctg gtgttctctc ctttattggc ctcttctacc ttatctggct caactggtac
     3481 tagcttgtag caccatccaa aggtcagtgg atatctgacc cctggccctg gtgtgtagtt
     3541 ctgctaatca gggaagtagc cttgtgtgtg gtagatccac agatcaagga tatcttgtct
     3601 tctttgggag tgaattagcc cttccaacta ctaagtttgt agtacatatt taacaaatac
     3661 aatttcttta aaatgaaaat aattcagagg aatcacaggt ttagagtaaa tgaaaccaca
     3721 ggtaattggc agtggcggtc cgtgcaaaag gccagaaccg taaaaaggcc gcgttgctgg
     3781 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     3841 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     3901 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     3961 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     4021 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     4081 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     4141 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     4201 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     4261 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     4321 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     4381 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     4441 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     4501 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     4561 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     4621 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     4681 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     4741 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     4801 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     4861 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     4921 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     4981 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     5041 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     5101 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     5161 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     5221 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     5281 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     5341 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     5401 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcgtgagcg
     5461 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     5521 gaaaagtgcc acctgacgtc aaacagctat gaccatggcg gcctcgaggg gcgcgccccc
     5581 agctggttct ttccgcctca gaagccatag agcccaccgc atccccagca tgcctgctat
     5641 tgtcttccca atcctccccc ttgctgtcct gccccacccc accccccaga atagaatgac
     5701 acctactcag acaatgcgat gcaatttcct cattttatta ggaaaggaca gtgggagtgg
     5761 caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc tggcaactag
     5821 aaggcacagt cgaggctgat cagcgagctc tagaactttg cctgtgtgct ccactcctag
     5881 ttcaattcct gctaaatcta ttataatgtc aaggcctctc actctctgat attcatttct
     5941 ttgcaagtta taaatactga ataataagat gacatgaact actactgcta gagattttcc
     6001 acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac
     6061 acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt
     6121 agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta caagcaaaaa
     6181 gcagatcttg tcttcgttgg gagtgaatta gcccttccag tccccccttt tcttttaaaa
     6241 agtggctaag atctacagct gccttgtaag tcattggtct taaagtgcgg atccttcgaa
     6301 ctagtttacg cgtgggggcg gccaaactta cgcacaagag ttccgtagct gttcaagttt
     6361 gtgtttcaac tgttctcgtc gtttccgcaa caagtcctct tcagaaatga gcttttgctc
     6421 ctctgcttgg acggacagga tgtatgctgt ggctttttta aggataacta ccttgggggc
     6481 cttttcattg ttttccaact ccgggatctg gtcacgcagg gcaaaaaagc tccgttttag
     6541 ctcgttcctc ctctggcgct ccaagacgtt gtgtgttcgc ctcttgacat tctcctcggt
     6601 gtccgaggac ctggggctgg tgcattttcg gttgttgctg atctgtctca ggactctgac
     6661 actgtccaac ttgaccctct tggcagcagg atagtccttc cgagtggagg gaggcgctgc
     6721 gtagttgtgc tgatgtgtgg agacgtggca cctcttgagg accagtgggc tgtgaggagg
     6781 tttgctgtgg cctccagcag aaggtgatcc agactctgac cttttgccag gagcctgcct
     6841 cttttccaca gaaacaacat cgatttcttc ctcatcttct tgttcctcct cagagtcgct
     6901 gctggtggtg ggcggtgtct cctcatggag caccaggggc tcggggctgc cctgcgggga
     6961 ggactccgtc gaggagagca gagaatccga ggacggagag aaggcgctgg agtcttgcga
     7021 ggcgcaggac ttgggcgagc tgctgtcgtt gagagggtag gggaagacca ccgaggggtc
     7081 gatgcactct gaggcggcgg cgctcagatc ctgcaggtac aagctggagg tggagcagac
     7141 gctgtggccg cgggcggggt tcgggctgcc gctgtctttg cgcgcagcct ggtaggaggc
     7201 cagcttctct gagacgagct tggcggcggc cgagaagccg ctccacatac agtcctggat
     7261 gatgatgttt ttgatgaagg tctcgtcgtc cgggtcgcag atgaaactct ggttcaccat
     7321 gtctcctccc agcagctcgg tcaccatctc cagctggtcg gccgtggaga agctcccgcc
     7381 accgccgtcg ttgtctcccc gaagggagaa gggtgtgacc gcaacgtagg agggcgagca
     7441 gagcccggag cggcggctag gggacagggg cggggtgggc agcagctcga atttcttcca
     7501 gatatcctcg ctgggcgccg ggggctgcag ctcgctctgc tgctgctgct ggtagaagtt
     7561 ctcctcctcg tcgcagtaga aatacggctg caccgagtcg tagtcgaggt catagttcct
     7621 gttggtgaag ctaacgttga ggggcatggc gatcgcgaat tcgaagcttg agctcgactg
     7681 cagaattcgc gatcgcggtg tggccatatt atcatcgtgt ttttcaaagg aaaaccacgt
     7741 ccccgtggtt cggggggcct agacgttttt ttaacctcga ctaaacacat gtaaagcatg
     7801 tgtaccgagg ccccagatca gatcccatac aatggggtac cttctgggca tccttcagcc
     7861 ccttgttgaa tacgcttgag gagagccatt tgactctttc cacaactatc caactcacaa
     7921 cgtggcactg gggttgtgcc gcctttgcag gtgtatctta tacacgtggc ttttggccgc
     7981 agaggcacct gtcgccaggt ggggggttcc gctgcctgca aagggtcgct acagacgttg
     8041 tttgtcttca agaagcttcc agaggaactg cttccttcac gacattcaac agaccttgca
     8101 ttcctttggc gagaggggaa agacccctag gaatgctcgt caagaagaca gggccaggtt
     8161 tccgggccct cacattgcca aaagacggca atatggtgga aaataacata tagacaaacg
     8221 cacaccggcc ttattccaag cggcttcggc cagtaacgtt aggggggggg gagggagagg
     8281 ggcggatcgt ttaaacgggt catatccact gtctgggatt taaaaatgcc tcttcatgtg
     8341 taaggcgagg tggtccgacc tggaaaatgc tcggtcgcat ttttggcact ggaacgggcg
     8401 gtgccccgtg tgtttacggt agtgcctggt cagttcatct gagcgggcga atttccatcc
     8461 acagccgtcc cagtcacagt ggtaaggttt ctcacctgtg tgggttcgca ggtgtgcctt
     8521 gagatgggaa ctctttgtgt aggttttgcc gcagcccgcg taatcacaag tgtgggtggc
     8581 ggtccttttc cggggccacg atcgtcttcc cctctttggc ttgggctcct ctggcatgca
     8641 ggaaccgggt ggcatgagct cttggtaatg gagcggcggg acttgcggct gcatctgatc
     8701 gggcaggaag gatgggtaat tgggccccgg gtggggatgg aagccgggag gaagcggcag
     8761 ggcagggtga cagtccctgc tgctcagcac ttcctcaaga cccagggtcg gggtagtcct
     8821 gctggggagc tgccgcccca gggggaagtc gtgtgcagcc ggccggtggc cattgctgag
     8881 agggggtcca gcgcccaagt gggtgcacga agagaccgcc tcctgcgtga tcttggggca
     8941 cgtgc
//
