LOCUS       sequence-181039-        1029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Huntingtin Chromobody 2: ""iVHH4"" intrabody recognizing amino acids
            49-148 of huntingtin, fused to eYFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Maiuri T, Mocle AJ, Hung CL, Xia J, van Roon-Mom WM, Truant R
  TITLE     Huntingtin is a scaffolding protein in the ATM oxidative DNA damage 
            response complex.
  JOURNAL   Hum Mol Genet. 2016 Dec 25. pii: ddw395. doi: 10.1093/hmg/ddw395.
  PUBMED    28017939
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mol 
            Genet. 2016 Dec 25. pii: ddw395. doi: 10.1093/hmg/ddw395."
COMMENT     Sequence Label: HCB2
FEATURES             Location/Qualifiers
     source          1..1029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(25..46)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(join(181..693,694..696,697..699))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     374..393
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     633..654
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gccgagngtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc
       61 gttggggtct ttgctcaggg cggactggta gctcaggtag tggttgtcgg gcagcagcac
      121 ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc
      181 ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat
      241 gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc
      301 ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt
      361 cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac
      421 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
      481 ggcgaagcac tgcaggccgt agccgaaggt ggtcacgagg gtgggccagg gcacgggcag
      541 cttgccggtg gtgcagatga acttcagggt cagcttgccg tangtggcat cgccctcgcc
      601 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
      661 caccaccccg gtgaacagct cctcgccctt gctcaccatg gatcctcctg aggagacggt
      721 gacctgggtc ccctggcccc agtagtcata atacccatta caatagtcgc tatagggagc
      781 gcggactgtc gcacagtaat aaacggccgt gtcctcangt ttcnngctgt tcatttgcag
      841 atacaccgtg ttcttggcat tgtctctgga gatggtgaat cggcccttca cggagtctgc
      901 atagtatgtg ctaccatcac tactactaat acatgananc ccctcgcgct ccttccctgg
      961 ggcctggngg aaccagccta tggcataata atccaaagtg aatccagang ctgcacagga
     1021 nantctnag
//
