LOCUS       sequence-181105-       13028 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast expression of S. cerevisiae Rpb1 with an internal PreScission 
            protease site and 10xHis tag for purification of the linker-CTD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13028)
  AUTHORS   Sdano MA, Fulcher JM, Palani S, Chandrasekharan MB, Parnell TJ, 
            Whitby FG, Formosa T, Hill CP
  TITLE     A novel SH2 recognition mechanism recruits Spt6 to the doubly 
            phosphorylated RNA polymerase II linker at sites of transcription.
  JOURNAL   Elife. 2017 Aug 16;6. pii: 28723. doi: 10.7554/eLife.28723.
  PUBMED    28826505
REFERENCE   2  (bases 1 to 13028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-08-16"; volume: "6. pii: 28723. doi"; pages: " 
            10.7554/eLife.28723"
COMMENT     Sequence Label: CPH3569 (YCp LEU2 Rpb1
            P1455_E1456InsGGGGGLEVL...
FEATURES             Location/Qualifiers
     source          1..13028
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(49..504)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(186..207)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     398..417
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     630..652
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     644..661
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     645..661
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     668..687
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        668..686
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6671..6694
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             6695..6724
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     promoter        complement(7836..7854)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7875..7891)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7875..7891)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7888..7910)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7899..7915
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7923..7929,7930..7947,7948..7953))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7968..7989
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8106..8123)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8277..8865)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8357..8376)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9036..9827,9828..9896))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9659..9678
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9897..10001)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    10038..10541
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     10583..10601
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10639..10661)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10761..10780
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(11274..12368)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(12369..12776)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
ORIGIN
        1 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg
       61 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
      121 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
      181 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
      241 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
      301 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
      361 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
      421 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
      481 ttaatgcgcc gctacagggc gcgtccattc gccattcagg ctgcgcaact gttgggaagg
      541 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
      601 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
      661 tgaattgtaa tacgactcac tatagggcga attggagctc ttgggaagct tcagtaccgc
      721 gatttttcag ctaatggatc agatgatata taaacaaaaa gtgggataca gcatatcgac
      781 gcctccacta caaattaaca gaaggatgaa aaccaataag aaaatttcta aaagaagaag
      841 cctgaaaaat ctccatggtg ccttaaaagg actattgaaa gagtccggca agaagagtga
      901 gtccaaaata cgaaaacata gcgattgcaa tcctgttcat cgagtatatc cgccaaatat
      961 cgaaaaacgg aagaccaaaa aggacgatgg aatttcacgg ccaattgctg aaagaaatgg
     1021 tcatgtgtat ataatgtcca aggaaaacca catcatccct aaactaaccg atgatgaagt
     1081 catggagcgc cacaaactag cagacgaaaa catgagaaaa gtgtggtcca acatcataag
     1141 caaatacgag tctattgaag aacaaggaga cctcgtagac ctgaaaactg gtgaaatagt
     1201 tgaagataat gggcatatca agacgctgac ggccaacaac agcacgaaag ataaaagaac
     1261 aaaatacaca agcgtgctca gagacattat cgacatcagc gacgaggaag atggtgataa
     1321 aaatgatgaa tatactcttt gggctaacga tagcgaggca agcgattcag aggtggatgc
     1381 tgacaacgac actgaagagg aaaaagacga gaaattgata gacgcagatt tcaagaagta
     1441 cgaggccaaa ctctcgaaaa ggatattacg agattaaggt gctaaaatgc cgtatattcg
     1501 aatttcatta ccatcagaat atatatatat atatatgagt ctatattagg gactatcata
     1561 cggtaacccg gacctccatt acccgcatga ctttactcac taaagacgat tgtcggtttt
     1621 aaacattttt tttttttcat ttcgaccgtc ggttctcctt agtttgttca gtggcaacaa
     1681 gtggacaaac aagaagagcg tgcagcacca ttcaccgcca gctttgtatc gtcctttaga
     1741 gtgcagcaag aacattaatt gctctcacag aggtatcata agaacatccg attcgttcat
     1801 taactgaaat catatctgtg ttgttctgat cgactctctt tgttagtttt atcttccatt
     1861 tttttttatt cctttcattt tccaataaaa tttgaacagg atcgatataa acagataaaa
     1921 accctccaaa gttaaccatt tgtttatatt tgcttttctg cataagtttt cctagcgaat
     1981 tccctgatca actttcaagg aaaactaaaa ctactgtatt ataagaggtt ttttttttca
     2041 ttccagatta attttgaaat acgatatcct caagtttatc taccagaata tttgactaag
     2101 aaatcaaact ctgttaataa taatataatt ataaaaacct caactagaaa ctccaaaaaa
     2161 aaaaatttac cattttttac tttctatcct tgttaaccaa atttcaaaaa aattttacct
     2221 tttctttttc cagaagaggg accaatcata aagatagcaa taacacttta ccccaaaata
     2281 taaatcagac atggtaggac aacagtattc tagtgctcca ctccgtacag taaaagaggt
     2341 ccaattcggt cttttctcac ctgaagaagt tagagcaatc agtgtggcca aaattagatt
     2401 tccagagaca atggatgaaa cccagacgag agcgaaaatt ggtggtctaa acgaccctag
     2461 gttaggctct attgatcgta atctgaagtg tcaaacttgt caagagggta tgaacgaatg
     2521 tcctggtcat tttggtcaca tagatttagc aaaacctgta tttcatgttg gttttattgc
     2581 caaaattaag aaagtatgtg agtgtgtctg tatgcactgt ggtaagctat tactggatga
     2641 acataatgaa ttaatgagac aagctctagc aatcaaagac agtaaaaaaa ggtttgctgc
     2701 aatttggact ttatgtaaaa caaaaatggt ctgcgaaaca gatgtccctt ctgaagatga
     2761 tcctactcag ctcgtatcaa ggggaggttg tggtaataca cagcctacaa ttcgtaagga
     2821 tgggttgaaa ttagttggta gttggaaaaa agatagagcc acgggggatg cggatgaacc
     2881 agaactaaga gttttaagta cggaggaaat cttgaatatt tttaagcata tctcagtaaa
     2941 agacttcact agtttgggtt tcaacgaagt tttttctcgt ccagaatgga tgattttaac
     3001 atgccttcct gtcccaccac caccggtgcg tccatccatt tccttcaatg aatctcaaag
     3061 aggtgaggat gatttaacct ttaaacttgc tgatatttta aaagctaata ttagtttgga
     3121 aacactagag cataacggtg ctccacatca tgctattgaa gaagcagaga gtttattaca
     3181 atttcatgtt gccacttata tggataatga tattgctggt caaccacaag ctctgcagaa
     3241 gtccggccgt cccgttaaat ctattcgtgc tcgtttgaag ggtaaagagg gtcgtatcag
     3301 aggtaattta atgggtaagc gtgtggattt ttcggcaaga actgttattt ctggtgatcc
     3361 taatttggaa ttagaccaag tcggtgttcc aaaatctatt gccaagactt taacataccc
     3421 agaagtggtc acaccatata acatagatcg tctgacgcaa cttgttagga atggaccaaa
     3481 tgagcacccc ggtgccaaat acgtcattcg tgatagcgga gaccgtatag atttaagata
     3541 cagtaaaagg gcaggtgata ttcaattaca gtatgggtgg aaagttgaac gtcatattat
     3601 ggacaatgat ccagttttat tcaaccgtca accttcgttg cacaaaatgt ccatgatggc
     3661 ccacagagta aaagttattc catattctac atttagattg aatttgtccg ttacatctcc
     3721 atacaatgcc gatttcgacg gtgacgaaat gaatcttcac gttcctcagt ctgaggaaac
     3781 aagggcggaa ctttctcaat tatgtgctgt tcctctgcaa attgtttcac cacaatctaa
     3841 caaaccttgt atgggtattg ttcaagatac tttgtgtggt attcgtaaac tgacattaag
     3901 agatacattt atagaacttg atcaagtttt gaatatgctt tattgggttc cagattggga
     3961 tggtgttatt ccgacacctg caattatcaa gcccaaacct ttgtggtccg gtaaacaaat
     4021 cttgtctgtg gctatcccaa acggtattca tttacaacgt tttgatgagg gcactactct
     4081 gctttctcca aaggataatg gtatgcttat tattgacggt caaatcattt ttggtgtagt
     4141 agagaaaaaa accgttggat cctccaatgg tggtttaatt catgttgtta cgagagaaaa
     4201 gggacctcaa gtttgtgcta agttgtttgg taacatacag aaagttgtta acttttggtt
     4261 actacataat gggttttcaa caggtattgg tgataccatt gcggacggcc caacaatgag
     4321 ggaaattaca gagacaattg cagaggctaa aaagaaagtt ttggatgtta cgaaagaagc
     4381 ccaggcaaac ttattgactg ctaaacatgg tatgactctc cgtgagtctt ttgaggataa
     4441 cgttgttcgg ttcctaaatg aagcaagaga taaggcaggt cgtttagctg aagtcaattt
     4501 gaaagatttg aacaatgtga aacaaatggt tatggcaggt tccaagggtt catttattaa
     4561 tatcgcgcaa atgtcagctt gtgtaggaca gcaatctgtt gaaggtaaac gtattgcttt
     4621 tgggttcgtt gatcgtacct tacctcattt ctctaaagat gattactccc cagagtctaa
     4681 aggttttgtt gagaactcat atttgagagg tttgacccca caagaatttt ttttccatgc
     4741 aatgggtggt cgtgaaggtc ttatcgatac cgccgtcaaa acagccgaaa caggttatat
     4801 tcaacgtcgt ttagtgaaag ctctagaaga tatcatggtt cattacgata acaccacaag
     4861 aaactcattg ggtaacgtta ttcagtttat ttatggtgaa gatggtatgg atgctgcgca
     4921 tattgaaaag caatcgctag atactattgg tggctccgat gcagcttttg aaaagagata
     4981 cagagttgat ttattgaata cagaccatac ccttgatccc tcactattgg aatccggatc
     5041 tgagatactt ggcgatttga aacttcaagt tctcctggat gaagaataca aacaattagt
     5101 gaaagatcgt aaatttttga gggaagtttt tgttgatggt gaagcaaact ggccattacc
     5161 agtcaacata agacgtatta ttcaaaatgc tcaacaaact ttccacatag atcatacgaa
     5221 accatctgat ttaacaatca aagacatcgt tcttggtgta aaggatttgc aagaaaactt
     5281 attagtgttg cgtggtaaga atgaaattat acaaaatgcc cagcgagatg cagttacatt
     5341 gttctgctgt ttattacgtt cccgtttggc cacacgtaga gttctacaag agtacagact
     5401 aacaaaacag gcattcgatt gggtattaag taatatcgag gcacaattcc tccgttctgt
     5461 tgttcaccct ggtgaaatgg ttggtgttct agcagcccaa tccattggtg aaccagccac
     5521 acaaatgacc cttaacacct tccattttgc tggtgttgct tccaaaaaag ttacttctgg
     5581 tgtcccccgt ttaaaggaaa ttttgaatgt ggccaaaaac atgaaaaccc cttccttgac
     5641 tgtatactta gagcccgggc atgctgccga tcaagaacaa gcgaagttga tcagatctgc
     5701 tatcgagcat accactttaa agagtgtcac tattgcttca gaaatttact atgatcctga
     5761 tccacgttcc acagttattc cagaagatga agaaattatc caacttcatt tctcattatt
     5821 ggatgaagag gctgaacaat cttttgacca acaatcacct tggttattac gtctggaact
     5881 ggatcgtgca gcaatgaatg ataaagactt aacaatgggt caggttggtg aaagaatcaa
     5941 gcaaacattc aaaaatgatt tgtttgttat ctggtctgaa gacaacgatg agaagttgat
     6001 catccgttgt cgtgttgttc gtccaaagtc actagatgct gagactgaag cagaagaaga
     6061 tcatatgttg aagaaaattg agaacacaat gttagagaat attacattac gtggtgtaga
     6121 gaacatcgag cgtgttgtca tgatgaaata tgaccgtaaa gtaccaagtc caactggtga
     6181 atacgttaag gaacctgaat gggtgttgga aacagatggt gttaacttat ctgaagttat
     6241 gactgttcct ggtatcgacc caaccagaat ctataccaac tccttcattg atataatgga
     6301 agttctaggt attgaagctg gtcgtgcagc cttgtataaa gaagtttaca atgttattgc
     6361 ttctgatggt tcgtatgtta actaccgtca tatggctttg ttagtcgatg ttatgacaac
     6421 ccaaggtggc ttaacttctg ttactcgtca tggtttcaac agatcaaata caggtgcctt
     6481 aatgagatgt tcatttgaag aaactgtcga aattttgttt gaagctggtg cttcagccga
     6541 attagatgat tgtcgtggtg tttcggaaaa tgtcattctt ggtcaaatgg ctccaatcgg
     6601 taccggtgca tttgatgtga tgatcgatga ggagtcactg gtaaaataca tgccaggagg
     6661 aggaggagga ctagaagttc tgttccaggg gccccaccac caccaccacc atcatcatca
     6721 tcatgaacaa aaaataactg agattgaaga cggacaagat ggtggcgtca caccatacag
     6781 taacgaaagt ggtttggtca atgcagatct tgacgttaac gatgagctaa tgttttcacc
     6841 tctggttaaa tcgggttcaa atgacgctat ggctggagga tttacagcgt acggtggtgc
     6901 tgattatggt gaagccacgt ctccatttgg tgcttatggt gaagcaccta catctcccgg
     6961 atttggagtc tcctcaccag gcttttctcc aacttcccca acatactctc ctacctctcc
     7021 agcgtactca ccaacatcac catcgtactc accaacatca ccatcgtact cgccaacatc
     7081 accatcgtac tcacctacat caccatcgta ttcaccaacg tcaccatcat attcgccaac
     7141 gtcaccatca tattcgccaa cgtcgccatc gtattctcca acgtcaccat cgtattcgcc
     7201 aacgtcgcct tcctactctc ccacgtcgcc aagctacagc cctacgtctc cttcttattc
     7261 tcctacatct ccatcatact ctcctacgtc accaagttac agcccaacgt caccaagtta
     7321 cagcccaacg tctccagcct attccccaac atcaccaagt tatagtccta catcgccttc
     7381 atactctcca acatcaccat cctattcccc aacatcacct tcttactctc ccacctctcc
     7441 aaactatagc cctacttcac cttcttactc cccaacatct ccaggctaca gcccaggatc
     7501 tcctgcatat tctccaaagc aagacgaaca aaagcataat gaaaatgaaa attccagatg
     7561 atatagtata tcatccttac gtatttgacg ttattacatt atatatagtt tctcaaataa
     7621 tatttctagt ttatttttgt atcataataa aaacgtatac caaatatacc attatttttc
     7681 atagcattat ggtagggata gggaatcaag taactaattt atatccgcag agcattggga
     7741 aaaccaacgg cgctagtaaa tgcatttaaa ttacgtccgt ccaacttcta agcttaaggg
     7801 tttctcgagg gggggcccgg taccagcttt tgttcccttt agtgagggtt aatttcgagc
     7861 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     7921 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     7981 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     8041 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     8101 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     8161 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8221 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8281 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8341 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8401 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8461 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8521 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8581 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8641 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8701 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8761 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8821 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     8881 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     8941 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     9001 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     9061 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     9121 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     9181 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     9241 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     9301 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     9361 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     9421 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     9481 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     9541 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     9601 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     9661 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     9721 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     9781 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     9841 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     9901 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     9961 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    10021 ccacctgggt ccttttcatc acgtgctata aaaataatta taatttaaat tttttaatat
    10081 aaatatataa attaaaaata gaaagtaaaa aaagaaatta aagaaaaaat agtttttgtt
    10141 ttccgaagat gtaaaagact ctagggggat cgccaacaaa tactaccttt tatcttgctc
    10201 ttcctgctct caggtattaa tgccgaattg tttcatcttg tctgtgtaga agaccacaca
    10261 cgaaaatcct gtgattttac attttactta tcgttaatcg aatgtatatc tatttaatct
    10321 gcttttcttg tctaataaat atatatgtaa agtacgcttt ttgttgaaat tttttaaacc
    10381 tttgtttatt tttttttctt cattccgtaa ctcttctacc ttctttattt actttctaaa
    10441 atccaaatac aaaacataaa aataaataaa cacagagtaa attcccaaat tattccatca
    10501 ttaaaagata cgaggcgcgt gtaagttaca ggcaagcgat ccgtcctaag aaaccattat
    10561 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
    10621 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
    10681 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
    10741 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatcga
    10801 ctacgtcgtt aaggccgttt ctgacagagt aaaattcttg agggaacttt caccattatg
    10861 ggaaatggtt caagaaggta ttgacttaaa ctccatcaaa tggtcaggtc attgagtgtt
    10921 ttttatttgt tgtatttttt tttttttaga gaaaatcctc caatatataa attaggaatc
    10981 atagtttcat gattttctgt tacacctaac tttttgtgtg gtgccctcct ccttgtcaat
    11041 attaatgtta aagtgcaatt ctttttcctt atcacgttga gccattagta tcaatttgct
    11101 tacctgtatt cctttacatc ctcctttttc tccttcttga taaatgtatg tagattgcgt
    11161 atatagtttc gtctacccta tgaacatatt ccattttgta atttcgtgtc gtttctatta
    11221 tgaatttcat ttataaagtt tatgtacaaa tatcataaaa aaagagaatc tttttaagca
    11281 aggattttct taacttcttc ggcgacagca tcaccgactt cggtggtact gttggaacca
    11341 cctaaatcac cagttctgat acctgcatcc aaaacctttt taactgcatc ttcaatggcc
    11401 ttaccttctt caggcaagtt caatgacaat ttcaacatca ttgcagcaga caagatagtg
    11461 gcgatagggt tgaccttatt ctttggcaaa tctggagcag aaccgtggca tggttcgtac
    11521 aaaccaaatg cggtgttctt gtctggcaaa gaggccaagg acgcagatgg caacaaaccc
    11581 aaggaacctg ggataacgga ggcttcatcg gagatgatat caccaaacat gttgctggtg
    11641 attataatac catttaggtg ggttgggttc ttaactagga tcatggcggc agaatcaatc
    11701 aattgatgtt gaaccttcaa tgtagggaat tcgttcttga tggtttcctc cacagttttt
    11761 ctccataatc ttgaagaggc caaaacatta gctttatcca aggaccaaat aggcaatggt
    11821 ggctcatgtt gtagggccat gaaagcggcc attcttgtga ttctttgcac ttctggaacg
    11881 gtgtattgtt cactatccca agcgacacca tcaccatcgt cttcctttct cttaccaaag
    11941 taaatacctc ccactaattc tctgacaaca acgaagtcag tacctttagc aaattgtggc
    12001 ttgattggag ataagtctaa aagagagtcg gatgcaaagt tacatggtct taagttggcg
    12061 tacaattgaa gttctttacg gatttttagt aaaccttgtt caggtctaac actaccggta
    12121 ccccatttag gaccacccac agcacctaac aaaacggcat cagccttctt ggaggcttcc
    12181 agcgcctcat ctggaagtgg aacacctgta gcatcgatag cagcaccacc aattaaatga
    12241 ttttcgaaat cgaacttgac attggaacga acatcagaaa tagctttaag aaccttaatg
    12301 gcttcggctg tgatttcttg accaacgtgg tcacctggca aaacgacgat cttcttaggg
    12361 gcagacatta gaatggtata tccttgaaat atatatatat atattgctga aatgtaaaag
    12421 gtaagaaaag ttagaaagta agacgattgc taaccaccta ttggaaaaaa caataggtcc
    12481 ttaaataata ttgtcaactt caagtattgt gatgcaagca tttagtcatg aacgcttctc
    12541 tattctatat gaaaagccgg ttccggcgct ctcacctttc ctttttctcc caatttttca
    12601 gttgaaaaag gtatatgcgt caggcgacct ctgaaattaa caaaaaattt ccagtcatcg
    12661 aatttgattc tgtgcgatag cgcccctgtg tgttctcgtt atgttgagga aaaaaataat
    12721 ggttgctaag agattcgaac tcttgcatct tacgatacct gagtattccc acagttaact
    12781 gcggtcaaga tatttcttga atcaggcgcc ttagaccgct cggccaaaca accaattact
    12841 tgttgagaaa tagagtataa ttatcctata aatataacgt ttttgaacac acatgaacaa
    12901 ggaagtacag gacaattgat tttgaagaga atgtggattt tgatgtaatt gttgggattc
    12961 catttttaat aaggcaataa tattaggtat gtagatatac tagaagttct cctcgaccgg
    13021 tcgatatg
//
