LOCUS       sequence-181144-        1966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Linker to construct BB1 from PCR product with FS3 and FS4 (in with 
            BsaI, out with BbsI).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Sarkari P, Marx H, Blumhoff ML, Mattanovich D, Sauer M, Steiger MG
  TITLE     An efficient tool for metabolic pathway construction and gene 
            integration for Aspergillus niger.
  JOURNAL   Bioresour Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004.
  PUBMED    28533066
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bioresour 
            Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004."
COMMENT     Sequence Label: BB1_L_34_syn_BsaI
FEATURES             Location/Qualifiers
     source          1..1966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(41..57)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(41..57)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      105..136
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        137..239
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             240..1055
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(313..332)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1079..1106
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1118..1705)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1198..1217)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1745..1762
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1746..1762
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gatcggtcca cgatcagcta gattatctag tcagcttgat gtcatagctg tttcctgagg
       61 ctcaatactg accatttaaa tcatacctga cctccatagc agaaagtcaa aagcctccga
      121 ccggaggctt ttgacttgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa
      181 gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa
      241 tgagccatat tcaacgggaa acgtcttgct tgaagccgcg attaaattcc aacatggatg
      301 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
      361 atcgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg
      421 ttgccaatga tgttacagat gagatggtca ggctaaactg gctgacggaa tttatgcctc
      481 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
      541 tcccagggaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
      601 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
      661 ttaacggcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
      721 ttggtgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
      781 aaatgcataa actcttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
      841 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
      901 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
      961 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
     1021 tgcagtttca cttgatgctc gatgagtttt tctaatgagg acctaaatgt aatcacctgg
     1081 ctcaccttcg ggtgggcctt tctgcgttgc tggcgttttt ccataggctc cgcccccctg
     1141 acgagcatca caaaaatcga tgctcaagtc agaggtggcg aaacccgaca ggactataaa
     1201 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     1261 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     1321 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     1381 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     1441 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     1501 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     1561 cagtatttgg tatctgcgct ctgctgaagc cagttacctc ggaaaaagag ttggtagctc
     1621 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     1681 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatt ttctaccgaa gaaaggccca
     1741 cccgtgtaaa acgacggcca gtttatctag tcagcttgat tctagctgat cgtggaccgg
     1801 aaggtgagcc agtgagttga ttgcagtcca gttacgctgg agtctgaggc tcgtcctgaa
     1861 tgatatgcgg cctcgaattc gaagacgcgc tttgagaccg gatccatctc gagggtctca
     1921 cgctgcgtct tcgaattcgc gcgtgatctt acggcattat acgtat
//
