LOCUS       sequence-18116-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Zhou Z, Sim J, Griffith J, Reed R
  TITLE     Purification and electron microscopic visualization of functional 
            human spliceosomes.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Sep 17. 99(19):12203-7.
  PUBMED    12215496
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2002 Sep 17. 99(19):12203-7."
COMMENT     Sequence Label: AdML-M3
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(395..413)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(396..413)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(494..513)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     613..635
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(673..691)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        759..863
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgcgtcatct ggtcatctag gatctgatat catcgatgaa ttcgagctcg gtaccccgtt
       61 cgtcctcact ctcttccgca tcgctgtctg cgagggccag ctgttggggt gagtactccc
      121 tctcaaaagc gggcatgact tctgccctcg agttattaac cctcactaaa ggcagtagtc
      181 aagggtttcc ttgaagcttt cgtgctgacc ctgtcccttt tttttccaca gctgcaggtc
      241 gacgttgagg acaaactctt cgcggtcttt ccagtactct tggatccgat atccgtacac
      301 catcagggta cgagctagcc catggcgtac accatcaggg tacgactagt agatctcgta
      361 caccatcagg gtacggaatt ctctagagtc gagttctata gtgtcaccta aatcgtatgt
      421 gtatgataca taaggttatg tattaattgt agccgcgttc taacgacaat atgtccatat
      481 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
      541 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
      601 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
      661 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
      721 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
      781 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
      841 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattcc
//
