LOCUS       sequence-18119-e         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Luo MJ, Reed R
  TITLE     Splicing is required for rapid and efficient mRNA export in 
            metazoans.
  JOURNAL   Proc Natl Acad Sci U S A. 1999 Dec 21. 96(26):14937-42.
  PUBMED    10611316
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1999 Dec 21. 96(26):14937-42."
COMMENT     Sequence Label: AdML
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(285..303)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(286..303)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(384..403)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     503..525
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(563..581)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctaggatctg atatcatcga tgaattcgag ctcggtaccc cgttcgtcct cactctcttc
       61 cgcatcgctg tctgcgaggg ccagctgttg gggtgagtac tccctctcaa aagcgggcat
      121 gacttctgcc ctcgagttat taaccctcac taaaggcagt agtcaagggt ttccttgaag
      181 ctttcgtgct gaccctgtcc cttttttttc cacagctgca ggtcgacgtt gaggacaaac
      241 tcttcgcggt ctttccagta ctcttggatc ctctagagtc gagttctata gtgtcaccta
      301 aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc taacgacaat
      361 atgtccatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
      421 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
      481 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
      541 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
      601 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
      661 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
      721 taaatgcttc
//
