LOCUS       sequence-181209-         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses lambdaN+-L+-GST fusion protein for affinity purification 
            with ARiBo method.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Di Tomasso G, Lampron P, Dagenais P, Omichinski JG, Legault P
  TITLE     The ARiBo tag: a reliable tool for affinity purification of RNAs 
            under native conditions.
  JOURNAL   Nucleic Acids Res. 2011 Feb;39(3):e18. doi: 10.1093/nar/gkq1084. 
            Epub 2010 Nov 11.
  PUBMED    21071425
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-02"; volume: "39(3):e18. doi"; pages: " 
            10.1093/nar/gkq1084. Epub 2010 Nov 11"
COMMENT     Sequence Label: pET42a-lambdaN+-L+-GSH
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             130..783
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLDFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISILEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     741..763
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 atggggaatg caaaaacacg ccgccgcgaa cgtcgcgcag agaaacaggc tcaatggaaa
       61 gcagcaaatg gcgcaggtgc aggcgcaggt gcaggcgcag gtgcaggcgc aggtgcaggc
      121 gcaggtgcaa tgtcccctat actaggttat tggaaaatta agggccttgt gcaacccact
      181 cgacttcttt tggaatatct tgaagaaaaa tatgaagagc atttgtatga gcgcgatgaa
      241 ggtgataaat ggcgaaacaa aaagtttgaa ttgggtttgg actttcccaa tcttccttat
      301 tatattgatg gtgatgttaa attaacacag tctatggcca tcatacgtta tatagctgac
      361 aagcacaaca tgttgggtgg ttgtccaaaa gagcgtgcag agatttcaat ccttgaagga
      421 gcggttttgg atattagata cggtgtttcg agaattgcat atagtaaaga ctttgaaact
      481 ctcaaagttg attttcttag caagctacct gaaatgctga aaatgttcga agatcgttta
      541 tgtcataaaa catatttaaa tggtgatcat gtaacccatc ctgacttcat gttgtatgac
      601 gctcttgatg ttgttttata catggaccca atgtgcctgg atgcgttccc aaaattagtt
      661 tgttttaaaa aacgtattga agctatccca caaattgata agtacttgaa atccagcaag
      721 tatatagcat ggcctttgca gggctggcaa gccacgtttg gtggtggcga ccatcctcca
      781 aaatcggatt aa
//
