LOCUS       sequence-181519-        9812 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression for Cas13a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9812)
  AUTHORS   East-Seletsky A, O'Connell MR, Burstein D, Knott GJ, Doudna JA
  TITLE     RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas 
            Enzymes.
  JOURNAL   Mol Cell. 2017 May 4;66(3):373-383.e3. doi: 
            10.1016/j.molcel.2017.04.008.
  PUBMED    28475872
REFERENCE   2  (bases 1 to 9812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2017.04"; journalName: "Mol Cell"; date: 
            "2017-05-4- 4"; volume: "66"; issue: "3"; pages: "373-383.e3"
COMMENT     Sequence Label: p2CT-His-MBP-Rca_Cas13a_WT
FEATURES             Location/Qualifiers
     source          1..9812
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..111
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     345..362
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    377..516
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(442..461)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     602..624
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(618..809)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     2267..2286
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     2370..2389
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2370..2388
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             2477..2499
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2519..2536
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2546..3646
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     3620..3643
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             3701..3721
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(7711..7729)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      7725..7772
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(8138..8143,8144..8160,8161..8166))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(8193..8211)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8279..8383
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8384..8452,8453..9244)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8602..8621)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9415..9812,1..191)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
       61 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      121 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      181 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      241 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
      301 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
      361 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
      421 accgcaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
      481 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
      541 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
      601 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg
      661 cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg
      721 tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg
      781 ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc
      841 atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat
      901 gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg
      961 gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt
     1021 ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct
     1081 gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct
     1141 caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca
     1201 ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg
     1261 atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg
     1321 gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc
     1381 cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg
     1441 cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga
     1501 caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg
     1561 cataaatcgc cgtgacgatc agcggtccaa tgatcgaagt taggctggta agagccgcga
     1621 gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca
     1681 acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc
     1741 ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa
     1801 tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg
     1861 cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc
     1921 ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa
     1981 agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga
     2041 ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta
     2101 ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat
     2161 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
     2221 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
     2281 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
     2341 agaggatcga gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg
     2401 gtttccctct agtgccggct ccggagagct ctttaattaa gcggccgccc tgcaggactc
     2461 gagttctaga aataattttg tttaacttta agaaggagat atacatatga aatcttctca
     2521 ccatcaccat caccatggtt cttctatgaa aatcgaagaa ggtaaactgg taatctggat
     2581 taacggcgat aaaggctata acggtctcgc tgaagtcggt aagaaattcg agaaagatac
     2641 cggaattaaa gtcaccgttg agcatccgga taaactggaa gagaaattcc cacaggttgc
     2701 ggcaactggc gatggccctg acattatctt ctgggcacac gaccgctttg gtggctacgc
     2761 tcaatctggc ctgttggctg aaatcacccc ggacaaagcg ttccaggaca agctgtatcc
     2821 gtttacctgg gatgccgtac gttacaacgg caagctgatt gcttacccga tcgctgttga
     2881 agcgttatcg ctgatttata acaaagatct gctgccgaac ccgccaaaaa cctgggaaga
     2941 gatcccggcg ctggataaag aactgaaagc gaaaggtaag agcgcgctga tgttcaacct
     3001 gcaagaaccg tacttcacct ggccgctgat tgctgctgac gggggttatg cgttcaagta
     3061 tgaaaacggc aagtacgaca ttaaagacgt gggcgtggat aacgctggcg cgaaagcggg
     3121 tctgaccttc ctggttgacc tgattaaaaa caaacacatg aatgcagaca ccgattactc
     3181 catcgcagaa gctgccttta ataaaggcga aacagcgatg accatcaacg gcccgtgggc
     3241 atggtccaac atcgacacca gcaaagtgaa ttatggtgta acggtactgc cgaccttcaa
     3301 gggtcaacca tccaaaccgt tcgttggcgt gctgagcgca ggtattaacg ccgccagtcc
     3361 gaacaaagag ctggcaaaag agttcctcga aaactatctg ctgactgatg aaggtctgga
     3421 agcggttaat aaagacaaac cgctgggtgc cgtagcgctg aagtcttacg aggaagagtt
     3481 ggcgaaagat ccacgtattg ccgccactat ggaaaacgcc cagaaaggtg aaatcatgcc
     3541 gaacatcccg cagatgtccg ctttctggta tgccgtgcgt actgcggtga tcaacgccgc
     3601 cagcggtcgt cagactgtcg atgaagccct gaaagacgcg cagactaatt cgagctcgaa
     3661 caacaacaac aataacaata acaacaacct cgggatcgag gaaaacctgt acttccaatc
     3721 caatgcaatg cagattggaa aagttcaagg ccgcacgatt tccgagttcg gcgaccctgc
     3781 tggcggctta aaacgtaaaa tttcgacaga cggtaagaat cgcaaagagt tgcctgcaca
     3841 tcttagctca gatccgaagg ctctgatcgg tcagtggatc agtgggatcg ataaaatcta
     3901 tcgcaagcca gattctcgta agtcggacgg caaggctatt cacagcccca ctccgtcgaa
     3961 gatgcagttt gatgcacgtg acgacttagg tgaagccttc tggaaacttg ttagcgaagc
     4021 cgggttggcc caagattcgg attacgacca atttaaacgt cgtcttcatc catatgggga
     4081 caagttccaa cctgcagact cgggagctaa attgaagttc gaggcggacc caccagaacc
     4141 gcaagcgttt cacggccgct ggtacggtgc tatgagcaaa cgcggaaatg acgcgaaaga
     4201 gctggcagct gcattatacg agcatcttca tgtcgacgaa aagcgcatcg acggtcagcc
     4261 aaaacgcaac cccaagacgg ataaatttgc gcccggatta gtcgtggctc gcgctctggg
     4321 tattgagtcc tcggttttac cgcgtgggat ggcgcgtctt gcacgtaatt ggggcgagga
     4381 ggaaattcaa acttatttcg tcgtcgacgt cgcagctagc gtaaaagaag ttgcgaaggc
     4441 ggccgtatcg gccgctcagg ctttcgaccc tccacgtcaa gtttcgggcc gttcgctttc
     4501 acccaaggta ggttttgcct tagctgagca tctggaacgc gtcactggta gcaagcgttg
     4561 tagctttgac cctgctgcag gccccagtgt attggcgctg catgatgagg taaagaaaac
     4621 ttacaagcgt ttgtgcgcgc gtggtaagaa tgctgcgcgt gcgtttcccg ctgacaaaac
     4681 ggaattgtta gcattgatgc gccataccca tgaaaaccgc gtgcgtaacc agatggtgcg
     4741 tatgggacgc gtcagcgagt accgtgggca acaagccggt gacctggcgc agtcacatta
     4801 ttggacgagc gcaggacaaa ctgaaattaa ggagtctgaa atctttgtcc gtctttgggt
     4861 aggtgccttt gccttagctg ggcgttccat gaaagcatgg attgacccca tgggaaagat
     4921 tgtaaacaca gaaaagaatg accgtgattt gactgccgct gtgaatatcc gccaagtgat
     4981 ctccaataaa gaaatggtgg cagaagctat ggctcgtcgc ggtatttact ttggcgagac
     5041 tccggaattg gatcgcttgg gggcggaagg caatgaagga tttgtttttg cgttattacg
     5101 ttatttacgt gggtgtcgta accagacctt ccatctgggc gcacgtgctg gttttttaaa
     5161 agaaatccgc aaggagcttg aaaagacacg ttgggggaag gcaaaagagg cggagcacgt
     5221 tgtgttaaca gataagacgg tagctgcaat ccgtgcgatt attgacaatg atgcgaaggc
     5281 tttaggagca cgcctgctgg ccgacttatc cggagcattt gtagcgcact acgcatctaa
     5341 agagcatttt tcgaccttgt actctgagat cgttaaggct gtcaaggacg ctcccgaagt
     5401 ctcgtcagga ttgccccgct tgaaactgct gcttaagcgc gccgacgggg tgcgcgggta
     5461 tgttcatggg ttgcgtgaca ctcgtaagca tgctttcgcc accaaattgc ccccaccacc
     5521 ggcgccacgt gagcttgacg acccagctac caaagcacgt tatattgctt tgctgcgcct
     5581 gtatgacggt ccatttcgcg cctatgcgtc aggcatcaca gggacggctt tggccggacc
     5641 cgcggcgcgt gctaaggaag ctgccactgc ccttgcacaa agtgtcaacg ttaccaaagc
     5701 atattcagat gttatggagg ggcgctcttc acgcctgcgc ccgcccaacg acggtgagac
     5761 cctgcgtgag tacttaagtg ctttaacggg cgagactgcg accgaattcc gtgtccaaat
     5821 cggatacgag tcggattctg aaaacgcacg caaacaagcg gaatttattg agaattatcg
     5881 ccgtgatatg ttggctttta tgtttgagga ttacattcgc gctaaaggtt ttgattggat
     5941 cttaaaaatc gaaccgggag ccacagcaat gacccgtgca ccagtgctgc ctgaacctat
     6001 tgacacacgt ggacaatacg aacattggca ggcggcattg tatctggtta tgcattttgt
     6061 gcccgcgtcc gacgtaagta atcttcttca tcagttacgt aaatgggagg ctcttcaagg
     6121 taaatatgaa ttagtacagg atggcgacgc aaccgaccaa gcggatgcac gtcgtgaggc
     6181 tttggattta gttaaacgtt tccgcgacgt tttggtactt tttcttaaga caggagaggc
     6241 acgcttcgaa ggccgcgcgg ctccctttga tttgaagcct ttccgtgctt tgttcgcgaa
     6301 ccccgcgact tttgaccgcc tgttcatggc cacacctaca accgcacgtc cggcggaaga
     6361 tgaccccgaa ggtgacgggg caagcgaacc cgaacttcgc gtcgcacgca cgctgcgcgg
     6421 acttcgccag atcgcgcgct acaaccacat ggcagttttg tcggatcttt tcgcgaaaca
     6481 taaggtgcgc gatgaagaag tggcccgctt agccgaaatt gaggatgaga cgcaggaaaa
     6541 atcccagatt gttgcggcac aggaactgcg tacggatttg catgacaagg taatgaagtg
     6601 ccatccaaag acgattagtc ctgaagaacg tcagagttac gcggccgcca tcaagaccat
     6661 cgaggagcat cgttttctgg ttgggcgtgt gtacttggga gatcatttac gtttgcatcg
     6721 tctgatgatg gatgtgatcg gacgtttgat cgactatgca ggcgcctatg aacgtgatac
     6781 cggcacgttt cttattaatg cttcaaagca attgggtgct ggagccgact gggctgtcac
     6841 gatcgctggt gcggcgaaca cagacgctcg tacacaaacg cgtaaggacc ttgcgcactt
     6901 taatgtgttg gaccgtgcag atggcactcc cgatttaacc gcgttggtga accgcgctcg
     6961 tgagatgatg gcttacgacc gtaaacgcaa gaatgctgta cctcgctcaa ttttagatat
     7021 gctggctcgt ctgggattga ctttaaaatg gcaaatgaaa gatcatttgt tgcaagacgc
     7081 tactattacc caggcggcca tcaagcactt agacaaggtg cgcttgaccg tcggaggccc
     7141 tgccgcagta actgaagccc gtttcagcca agactacttg cagatggtag ctgctgtatt
     7201 taatggaagt gtacagaatc ctaagcctcg ccgtcgcgac gatggtgacg cctggcacaa
     7261 gccacccaag ccagccacgg cgcaaagtca acctgaccaa aagccaccca acaaagcgcc
     7321 ttccgctggt tctcgtcttc cgcctcccca agttggtgag gtgtacgaag gggtagttgt
     7381 gaaggtcatt gacacaggct cacttgggtt ccttgccgtg gagggggtcg caggcaatat
     7441 cggtcttcac atctcacgtc ttcgtcgtat tcgtgaagac gcgattattg taggacgtcg
     7501 ttatcgcttt cgcgtagaga tttatgtgcc tcctaagtcg aatactagca agttaaacgc
     7561 tgcggacctg gttcgtatcg attaataaca ttggaagtgg ataacggatc cgcgatcgcg
     7621 gcgcgccacc tggtggccgg ccggtaccac gcgtgcgcgc tgatccggct gctaacaaag
     7681 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
     7741 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc
     7801 cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg
     7861 agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga
     7921 aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa
     7981 tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca
     8041 tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc
     8101 tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc
     8161 tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
     8221 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     8281 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     8341 ataaccctga taaatgcttc aataacattg aaaaaggaag agtatgagta ttcaacattt
     8401 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     8461 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     8521 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     8581 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
     8641 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     8701 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     8761 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     8821 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     8881 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac
     8941 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     9001 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     9061 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     9121 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     9181 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     9241 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     9301 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     9361 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     9421 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     9481 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     9541 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     9601 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     9661 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     9721 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     9781 cgaactgaga tacctacagc gtgagctatg ag
//
