LOCUS       sequence-181561-        7942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of mouse Gfi1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7942)
  AUTHORS   Vo BT, Li C, Morgan MA, Theurillat I, Finkelstein D, Wright S, Hyle 
            J, Smith SM, Fan Y, Wang YD, Wu G, Orr BA, Northcott PA, Shilatifard
            A, Sherr CJ, Roussel MF
  TITLE     Inactivation of Ezh2 Upregulates Gfi1 and Drives Aggressive 
            Myc-Driven Group 3 Medulloblastoma.
  JOURNAL   Cell Rep. 2017 Mar 21;18(12):2907-2917. doi: 
            10.1016/j.celrep.2017.02.073.
  PUBMED    28329683
REFERENCE   2  (bases 1 to 7942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.02"; journalName: "Cell Rep"; date: 
            "2017-03-21- 21"; volume: "18"; issue: "12"; pages: "2907-2917"
COMMENT     Sequence Label: pT2K-CAGGS-mouse Gfi1-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..7942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     336..358
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     424..443
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     misc_feature    1789..2338
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1933..1950)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2160..2179
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(2341..2343,2344..2346,2347..3060)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2341..2343,2344..2346,2347..3057)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2386..2407)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2647..2666)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2994..3015
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3098..3117)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3163..3218
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3217..3236)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     misc_feature    join(3886..4073,4074..4085)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(4119..4135)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4119..4135)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4172..4192)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4172..4190)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4211..4227)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4211..4227)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4224..4246)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4235..4251
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4259..4265,4266..4283,4284..4289))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4304..4325
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4442..4459)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4613..5201)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4693..4712)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5372..6163,6164..6232))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5995..6014
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6233..6337)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6363..6818)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6500..6521)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6712..6731
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6945..6967
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6959..6976
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6960..6976
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6986..7005
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6986..7004
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg
       61 tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg
      121 acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc
      181 tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct
      241 tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg
      301 ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg
      361 cggctctagc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
      421 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc gatatcaccg
      481 gtaccatgcc gcgctcattc ctggtcaaga gcaagaaggc gcacagctat caccagccgc
      541 gttctccggg gccggactac tccctgcgcc tggagaccgt gcctgcgccg ggcagagcag
      601 agggcggcgc tgtgagtgca ggcgagtcga aaatggagcc ccgagagcgt ttgtcccccg
      661 actctcagct taccgaggct cccgacaggg cctccgcgtc ccccaacagc tgcgaaggca
      721 gcgtttgtga cccctgctcc gagttcgagg acttttggag gcccccttct ccctccgtgt
      781 ctccagcgtc ggagaagtca ctgtgccgct ctctggacga agcccagccc tacacgctgc
      841 ctttcaagcc ctatgcatgg agcggtcttg ctgggtctga cctgcggcac ctggtgcaga
      901 gctatcggca gtgcagcgcg ctggagcgca gcgcgggcct gagcctcttc tgcgagcgcg
      961 gctcggagcc gggccgcccg gcagcgcgct acggccccga gcaggctgcg ggcggagccg
     1021 gtgcgggaca gccagggagc tgcggggtcg ccgggggcgc caccagcgct gcgggcctgg
     1081 ggctctacgg cgacttcgcg cctgcggcgg ccgggctgta cgagcggccg agcacagcag
     1141 caggccggct gtaccaagat catggccacg agctgcacgc ggacaagagc gtgggcgtca
     1201 aggtggagtc ggagctgctt tgcacccgtc tgctgctggg cggcggctcc tacaaatgca
     1261 tcaaatgcag caaggtgttc tccacaccgc acgggctgga ggtgcacgtg cgccggtccc
     1321 acagcggcac aagacccttt gcgtgcgaga tgtgcggcaa gaccttcggg cacgcggtga
     1381 gcctggagca acacaaggca gtgcactccc aggaacgcag ctttgactgt aagatctgtg
     1441 gcaagagctt caagaggtca tccacgctgt ccacacatct gctcattcac tcggacaccc
     1501 ggccctatcc ctgtcagtac tgtggcaaaa gattccacca gaagtcagat atgaagaaac
     1561 acaccttcat ccacacaggt gagaagcccc acaaatgcca ggtgtgcggc aaagccttca
     1621 gtcagagctc caacctcatc actcatagca gaaagcacac aggcttcaag ccctttggct
     1681 gtgacctgtg tgggaagggc ttccagagga aggtggatct caggaggcac cgagagactc
     1741 agcatggact caaatgactc gagcgggatc aattccgccc cccccctaac gttactggcc
     1801 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
     1861 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
     1921 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
     1981 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
     2041 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
     2101 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
     2161 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
     2221 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
     2281 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgataatacc
     2341 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     2401 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     2461 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     2521 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     2581 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     2641 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     2701 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     2761 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     2821 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     2881 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     2941 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     3001 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     3061 agcggccgcg actctagagt cgagtctaga tatcagatct ttttccctct gccaaaaatt
     3121 atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt
     3181 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg
     3241 caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata
     3301 tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc
     3361 ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat
     3421 tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag
     3481 ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga
     3541 gatccctcga cctgcagccc aagcttctgc gaagatacgg ccacgggtgc tcttgatcct
     3601 gtggctgatt ttggactgtg ctgctcgcag ctgctgatga atcacatact tcctccattt
     3661 tcttccactg attgactgtt ataatttccc taatttccag gtcaaggtgc tgtgcattgt
     3721 ggtaatagat gtgacatgac gtcacttcca aaggaccaat gaacatgtct gaccaatttc
     3781 atataatgtg aaaacgattt tcataggcag aataaataac atttaaatta aactgggcat
     3841 cagcgcaatt caattggttt ggtaatagca agggaaaata gaatgaagtg atctccaaaa
     3901 aataagtact ttttgactgt aaataaaatt gtaaggagta aaaagtactt ttttttctaa
     3961 aaaaatgtaa ttaagtaaaa gtaaaagtat tgatttttaa ttgtactcaa gtaaagtaaa
     4021 aatccccaaa aataatactt aagtacagta atcaagtaaa attactcaag tactttacac
     4081 ctctggttct tgacccccta ccttcagcaa gcccagcaga tccactagtt ctagagcggc
     4141 cgccaccgcg gtggagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg
     4201 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     4261 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     4321 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     4381 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     4441 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     4501 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     4561 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     4621 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     4681 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     4741 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     4801 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     4861 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     4921 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     4981 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     5041 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     5101 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     5161 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     5221 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     5281 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     5341 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     5401 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     5461 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     5521 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     5581 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     5641 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     5701 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     5761 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     5821 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     5881 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     5941 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     6001 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     6061 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     6121 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     6181 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     6241 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     6301 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     6361 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     6421 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     6481 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     6541 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     6601 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     6661 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     6721 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     6781 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
     6841 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     6901 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     6961 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccaa
     7021 atagtaggaa ttacccacct gtacaagtgc tgaaaacttg gatgaataag cccgtttgcc
     7081 attttctact gctattttaa atcttttctg tatttgtctg catttgtctt tacccttgaa
     7141 taaatgtttt agttgttttt tttcaattat gactgtgttt aacagacatt attacaataa
     7201 tatgtaatag tagtacacac tattattatg taatagtact tgttgactgt atttgagaac
     7261 tggaccgagt gagtgttacg tcacccattc aaaatgactt acttctggct ccaacaaaat
     7321 gaagttaatt cagttgccat ttttcactgt atggacatcg ccgtgttgga gctagacgtt
     7381 gccaataagc aagaaagagc cgagatgcgt cgagtctgag tcaccgttcc tatggcaacc
     7441 cctctaacca atcagaagta agcttgttgg aagtccacag cctaccactt gaaagcgggc
     7501 tgcacaaaat ctgtcaaacg ttttgaacgt tggatgtgag agcacatact tttattaagg
     7561 catctggttg gtcagtttat aatatcaaca acttgggcta cagaaaagaa aagttattac
     7621 agaaattatg attaacaagt acatgttaaa taaagatttt aatatgaatg ccaccactgg
     7681 agcattcatg ccatttggag cttcttcctg tttggatcac tagaaggagg aggtcactca
     7741 ttacagttct catatacagt cgttggttgg ttggttggtt ggtagattga ttgattgatt
     7801 gattgattga ttgattgatt gattgattga ttgattgatt gattgattga ttgattgatt
     7861 ggtagtcaaa ataagaaata atttccacag attcattaca gaaatgatta aatgcataca
     7921 taaaaaactg gggggggggg gg
//
