LOCUS       sequence-181597-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of the photoconvertible protein mEos2 as an 
            Ensconsin fusion..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Mohr MA, Kobitski AY, Sabater LR, Nienhaus K, Obara CJ, 
            Lippincott-Schwartz J, Nienhaus GU, Pantazis P
  TITLE     Rational Engineering of Photoconvertible Fluorescent Proteins for 
            Dual-Color Fluorescence Nanoscopy Enabled by a Triplet-State 
            Mechanism of Primed Conversion.
  JOURNAL   Angew Chem Int Ed Engl. 2017 Jun 29. doi: 10.1002/anie.201706121.
  PUBMED    28661566
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2017 Jun 29. doi: 10.1002/anie.201706121."
COMMENT     Sequence Label: Ensconsin-18-mEos2
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..682)
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 cttatcgtct ggcattgtca ggcaatccag aatgagcaac agcatgctca tacagcttaa
       61 ccttgttgta atctttgtca tggcttaaaa tctcaatgca gtggtccaca aagtggtagc
      121 ctggtaactt gacacccttc tccttagctt tgtaagtagt tctgaagtca catcggtaat
      181 gggcatttcc ttcaagcaac aaagccatat gaatatcacc cgtcagcact ccatcacgca
      241 catacatttt ctcagtggag ggctcccatt tcagcgtctt cttctgcata actggaccat
      301 tggcgggaaa gttggtacca taaaatcgaa ctttattata gaaagtgtcc ccttccattg
      361 ttatgtcgtt tctggcaatg caaatgcccc cgtcttcgaa agtcaagctt cgttcccacg
      421 aatacccctt aggaaacgac tgcttaaaat agtcttgtat gttgtctgga tatttggcga
      481 ataccctgtt gccgtaatgg aatgcagtgg tcaggatatc aaaggcaaaa ggcagaggtc
      541 cgccctcttt gacttcaaga tccatactct gttttccctc aaaaggcttg cctgtaccat
      601 ctccgtcgat cacaaagtgg tgcccgttta cgttgccttc catacggagt ttgatcttca
      661 tgtctggctt aatcgcactc atggtggcga ccggtggatc ccgggcccgc ggtaccccgc
      721 ttccgcctgc gctgcctata acttctgcag tctgctgtgt ctgaacacca tctacctgag
      781 gcaggggccc aagtgtccct tcatcatcaa aggccaaaat tggattcaaa ggaatttctg
      841 ggctctcact gttggtgaca tctaatctgg atggtttaga tccaatgggt aagtttataa
      901 tttcttcaaa attttcattc tgaacagata caccattttc atttggttgt ttttccaaat
      961 cgggagtgct c
//
