LOCUS       sequence-181713-        4638 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression in eukaryotic cells .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Gao W, Yang J, Liu W, Wang Y, Shao F
  TITLE     Site-specific phosphorylation and microtubule dynamics control Pyrin
            inflammasome activation.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Aug 16;113(33):E4857-66. doi: 
            10.1073/pnas.1601700113. Epub 2016 Aug 1.
  PUBMED    27482109
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1601700113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-08-16- 16"; volume: "113"; issue: "33"; pages: 
            "E4857-66"
COMMENT     Sequence Label: pCS2-3HA-Mefv iso1
FEATURES             Location/Qualifiers
     source          1..4638
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        100..1084
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        1119..1137
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1119..1136
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             1175..1255
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     polyA_signal    3729..3863
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3753..3772)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3807..3826
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3976..3996)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3976..3994)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4015..4031)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4015..4031)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4028..4050)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4039..4055
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4063..4069,4070..4087,4088..4093))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4108..4129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4246..4263)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(4497..4516)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg
       61 ggaagggcga tcggtgcggg cctcttcgct attacgccag tcgaccgcca attcaatatg
      121 gcgtatatgg actcatgcca attcaatatg gtggatctgg acctgtgcca attcaatatg
      181 gcgtatatgg actcgtgcca attcaatatg gtggatctgg accccagcca attcaatatg
      241 gcggacttgg caccatgcca attcaatatg gcggacctgg cactgtgcca actggggagg
      301 ggtctacttg gcacggtgcc aagtttgagg aggggtcttg gccctgtgcc aagtccgcca
      361 tattgaattg gcatggtgcc aataatggcg gccatattgg ctatatgcca ggatcaatat
      421 ataggcaata tccaatatgg ccctatgcca atatggctat tggccaggtt caatactatg
      481 tattggccct atgccatata gtattccata tatgggtttt cctattgacg tagatagccc
      541 ctcccaatgg gcggtcccat ataccatata tggggcttcc taataccgcc catagccact
      601 cccccattga cgtcaatggt ctctatatat ggtctttcct attgacgtca tatgggcggt
      661 cctattgacg tatatggcgc ctcccccatt gacgtcaatt acggtaaatg gcccgcctgg
      721 ctcaatgccc attgacgtca ataggaccac ccaccattga cgtcaatggg atggctcatt
      781 gcccattcat atccgttctc acgcccccta ttgacgtcaa tgacggtaaa tggcccactt
      841 ggcagtacat caatatctat taatagtaac ttggcaagta cattactatt ggaagtacgc
      901 cagggtacat tggcagtact cccattgacg tcaatggcgg taaatggccc gcgatggctg
      961 ccaagtacat ccccattgac gtcaatgggg aggggcaatg acgcaaatgg gcgttccatt
     1021 gacgtaaatg ggcggtaggc gtgcctaatg ggaggtctat ataagcaatg ctcgtttagg
     1081 gaaccgccat tctgcctggg gacgtcggag caagcttgat ttaggtgaca ctatagaata
     1141 caagctactt gttctttttg caggatccac catgtaccca tacgatgttc cagattacgc
     1201 ttatccttat gacgtacctg actatgcata cccttatgat gtaccagact acgctttaat
     1261 taagatggcc aagacccttg gtgatcatct gctaaacacc ctggaggagc tgctgcccta
     1321 tgactttgag aagttcaagt ttaagctaca gaacaccagc ctggagaagg gccactctaa
     1381 gatcccccgg ggccatatgc agatggccag gccagtaaag ctggccagcc ttctgataac
     1441 ctactacggg gaggagtatg ctgtaaggct gaccctgcag atcctgcggg ccaccaacca
     1501 gcgccaacta gcagaggagc ttcgcaaagc cacaggcaca gaacatttaa ttgaagaaaa
     1561 tagagttggt ggttctgtcc agtcttctgt agagaataaa gccaagagtg tgaaggtacc
     1621 agatgtccct gaaggtgatg ggacacagca aaacaatgat gaatcagaca cccttccatc
     1681 cagccaggct gaagtgggga agggacccca gaagaagtca ctgaccaaaa ggaaggatca
     1741 gaggggcccc gagagcctgg actcacagac caagccatgg acaaggagca cagcgcctct
     1801 ctataggaga acccagggca cccagtcccc aggggataag gagagcacag caagtgccca
     1861 gctccgcagg aatgtcagct ctgcagggag gcttcaagga ctttacaaca atgccccagg
     1921 gaggagagaa agcaagaaag ccgaagtgta tgtatatttg ccttcaggaa agaagcgacc
     1981 caggagtctt gaaattacca cttattcaag agaaggagaa cccccaaatt cagaagttct
     2041 tccaactcaa gaggaaacaa gaaatgggag tctcattcgc atgagaacag ctactctgaa
     2101 tggaaggact acgggggctt tagaaaaggg cacagggatt ccagagcatt ccatggtgct
     2161 ggatgaaaaa acattcagaa acatgtcttc caaaacatcg ttgattgggg aggagagatg
     2221 ccctacatca tggacggaaa atggaaatgg gagtccagaa accacagagt cctcgggaga
     2281 gacggctggc agcatactct ctgatccaga agtgcctttg tcactatgtg aaaaaccagc
     2341 taaaactcca gaagacccag catccttagg acaggccgct tgtgaaggta ccctccttca
     2401 ggtctcaaga aaatgtgatg caggcccaga aaaatgcaca tttacacatc ttgcactaca
     2461 ggttgcatca aatttcggaa ggtcacagga caaggctgtg tgccctcttt gccacaccca
     2521 ggaaggagac ctgcgtggtg atacctgtgt gcaaagttcc tgtagctgct ccattgctcc
     2581 tggggatccc aaggcctccg gcagatgctc catatgcttt cagtgccaag gcttacttgc
     2641 cagaaagagc tgcgaagccc agagccccca gtccctacca cagtgccctc gtcacatgaa
     2701 gcaggtgctg ctgcttttct gtgaggacca cagggagccc atctgcctca tctgcaggct
     2761 gagcctggag catcaaggac atcgagtgcg ccccatagag gaggctgcac tagagtacaa
     2821 ggagcagatc cgggagcagc tggagcgcct gcgggagatg aggggatatg tggaggagca
     2881 caggctacag ggagacaaga aaacagacga tttcctgaaa caaacagaaa tccagaagca
     2941 gaagatttca tgtccacttg agaagctata ccaactcctg gagaagcaag aacaactctt
     3001 tgtgacctgg ctacaggagc taagccagac catcagcaag gtcagagaga catactacac
     3061 ccgggtttcc ctactggatg agatgattga agaactagag gccaagcaag accagccaga
     3121 gtgggacctc atgcaggaca ttggaatcac cttgcacagg gctaagatga tgtctgcctc
     3181 tgagctgttg gacactcctc caggtgttaa agaaaagctt catctgctct accagaagtc
     3241 aaagtctgtg gagaagaata tgcagtgttt ctcagaaatg ctgagctctg aaatggcatt
     3301 cagcgcttca gatgtagcaa aatgggaagg gcgccagcct agcgccacac aggtccaggg
     3361 cttggttccc accgttcacc taaagtgtga tggtgcacac acacaggact gtgatgtagt
     3421 attttaccca gaacgggaag ctggaggatc agaacctaaa gattaccttc atcctcagcc
     3481 agctcaagac acacctgagc tacatgagat ccattctcgg aacaacaaaa gaaaatttaa
     3541 atctttcctg aaatggaaac cttcattcat tgggagcacc ttgggagaag cccacagatc
     3601 cgcactccca gctaggcatt cttcttcctg cagttaccgt gatttggacc aagcagctgc
     3661 tcaccccaac ctcatcttct cgatgatatg aggcgcgcct ctagaactat agtgagtcgt
     3721 attacgtaga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     3781 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     3841 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     3901 agggggaggt gtgggaggtt ttttaattcg cggccgcggc gccaatgcat tgggcccggt
     3961 acccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata
     4021 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccgggag
     4081 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     4141 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     4201 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     4261 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     4321 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     4381 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     4441 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     4501 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     4561 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     4621 ctgtaggctg tctcttat
//
