LOCUS       sequence-181742-        4901 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human RPS6KA1 [NM_002953.3] with stop codon 
            (for native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4901)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E289 Hs.RPS6KA1
FEATURES             Location/Qualifiers
     source          1..4901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2392..2491)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2868..3659
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3752..4340
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4241..4260
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4494..4511
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4670..4697)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4789..4875)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgcc gctcgcccag ctcaaggagc cctggccgct catggagcta gtgccgctgg
      241 acccggagaa tggacagacc tcaggggaag aagctggact tcagccgtcc aaggatgagg
      301 gcgtcctcaa ggagatctcc atcacgcacc acgtcaaggc tggctctgag aaggctgatc
      361 catcccattt cgagctcctc aaggttctgg gccagggatc ctttggcaaa gtcttcctgg
      421 tgcggaaagt cacccggcct gacagtgggc acctgtatgc tatgaaggtg ctgaagaagg
      481 caacgctgaa agtacgtgac cgcgtccgga ccaagatgga gagagacatc ctggctgatg
      541 taaatcaccc attcgtggtg aagctgcact atgccttcca gaccgagggc aagctctatc
      601 tcattctgga cttcctgcgt ggtggggacc tcttcacccg gctctcaaaa gaggtgatgt
      661 tcacggagga ggatgtgaag ttttacctgg ccgagctggc tctgggcctg gatcacctgc
      721 acagcctggg tatcatttac agagacctca agcctgagaa catccttctg gatgaggagg
      781 gccacatcaa actcactgac tttggcctga gcaaagaggc cattgaccac gagaagaagg
      841 cctattcttt ctgcgggaca gtggagtaca tggcccctga ggtcgtcaac cgccagggcc
      901 actcccatag tgcggactgg tggtcctatg gggtgttgat gtttgagatg ctgacgggct
      961 ccctgccctt ccaggggaag gaccggaagg agaccatgac actgattctg aaggcgaagc
     1021 taggcatgcc ccagtttctg agcactgaag cccagagcct cttgcgggcc ctgttcaagc
     1081 ggaatcctgc caaccggctc ggctccggcc ctgatggggc agaggaaatc aagcggcatg
     1141 tcttctactc caccattgac tggaataagc tataccgtcg tgagatcaag ccacccttca
     1201 agccagcagt ggctcagcct gatgacacct tctactttga caccgagttc acgtcccgca
     1261 cacccaagga ttccccaggc atccccccca gcgctggggc ccatcagctg ttccggggct
     1321 tcagcttcgt ggccaccggc ctgatggaag acgacggcaa gcctcgtgcc ccgcaggcac
     1381 ccctgcactc ggtggtacag caactccatg ggaagaacct ggtttttagt gacggctacg
     1441 tggtaaagga gacaattggt gtgggctcct actctgagtg caagcgctgt gtccacaagg
     1501 ccaccaacat ggagtatgct gtcaaggtca ttgataagag caagcgggat ccttcagaag
     1561 agattgagat tcttctgcgg tatggccagc accccaacat catcactctg aaagatgtgt
     1621 atgatgatgg caaacacgtg tacctggtga cagagctgat gcggggtggg gagctgctgg
     1681 acaagatcct gcggcagaag ttcttctcag agcgggaggc cagctttgtc ctgcacacca
     1741 ttggcaaaac tgtggagtat ctgcactcac agggggttgt gcacagggac ctgaagccca
     1801 gcaacatcct gtatgtggac gagtccggga atcccgagtg cctgcgcatc tgtgactttg
     1861 gttttgccaa acagctgcgg gctgagaatg ggctcctcat gacaccttgc tacacagcca
     1921 actttgtggc gcctgaggtg ctgaagcgcc agggctacga tgaaggctgc gacatctgga
     1981 gcctgggcat tctgctgtac accatgctgg caggatatac tccatttgcc aacggtccca
     2041 gtgacacacc agaggaaatc ctaacccgga tcggcagtgg gaagtttacc ctcagtgggg
     2101 gaaattggaa cacagtttca gagacagcca aggacctggt gtccaagatg ctacacgtgg
     2161 atccccacca gcgcctcaca gctaagcagg ttctgcagca tccatgggtc acccagaaag
     2221 acaagcttcc ccaaagccag ctgtcccacc aggacctaca gcttgtgaag ggagccatgg
     2281 ctgccacgta ctccgcactc aacagctcca agcccacccc ccagctgaag cccatcgagt
     2341 catccatcct ggcccagcgg cgagtgagga agttgccatc caccaccctg taatcaactt
     2401 tcttgtacaa agttggcatt ataggaaagc attgcttatc aatttgttgc aacgaacagg
     2461 tcactatcag tcaaaataaa atcattattt gcatgcaagc ttagatcctc tagaccagcc
     2521 aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga
     2581 aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
     2641 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     2701 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
     2761 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     2821 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg
     2881 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     2941 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     3001 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     3061 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     3121 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     3181 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     3241 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     3301 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     3361 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     3421 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     3481 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     3541 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     3601 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac
     3661 cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt
     3721 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     3781 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     3841 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3901 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3961 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     4021 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     4081 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     4141 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     4201 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     4261 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     4321 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     4381 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     4441 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     4501 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     4561 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     4621 acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc
     4681 cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg
     4741 ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact
     4801 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc
     4861 ctttcgtttt atttgatgcc tggcagttcc ctactctcgc g
//
