LOCUS       sequence-181753-        2062 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module A, Promoter: none, Gene: none, Terminator: none.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_A0000
FEATURES             Location/Qualifiers
     source          1..2062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        970..1074
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccacct gctcatgatc ggcgcgcgcg gccgcacaga gatttaaata
      901 gctccggtga cggacgtggg caggtgatga aaagccttag ggtcaggtgg cacttttcgg
      961 ggaaatgtgc gatgaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     1021 ctaatgagac aataaccctg ataaatgctt caattatatt gaaaaaggaa gagtatgagt
     1081 atccaacatt tccgtgtcgc ccttattccc ttttttgcgg ccttctgcct tcctgttttt
     1141 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcccgagtg
     1201 ggttacattg aactggatct caacagcggc aaaatcttag agagttttcg ccccgaagaa
     1261 cgcttcccaa tgatgagcac tttcaaagtt ctgctatgtg gcgcggtgtt atcccgtatt
     1321 gatgctgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1381 tattcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgtagt
     1441 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gattggagga
     1501 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1561 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1621 gcaatggcaa caactctccg caaactatta actggcgaac tacttactct tgcttcacgc
     1681 cagcaactca ttgactggat ggaggcggac aaagttgcag gaccacttct gcgctcggca
     1741 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1801 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1861 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1921 attaagcatt ggtaactgtc agacgaaggg aaataaatag tagcccgcct gatgtgcggg
     1981 cttttttttg gtggtggtta ccagtgtcgc ttagctaccg taggtcctct taggcggtcc
     2041 gattagcgac atagtcatca at
//
