LOCUS       sequence-181784-         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To fuse your protein of interest to the C-terminus of BioID2 and use
            in proximity-dependent biotin identification (BioID); MCS and a 25nm
            GS linker are present downstream of BioID2 and myc tags..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Kim DI, Jensen SC, Noble KA, Kc B, Roux KH, Motamedchaboki K, Roux 
            KJ
  TITLE     An improved smaller biotin ligase for BioID proximity labeling.
  JOURNAL   Mol Biol Cell. 2016 Feb 24. pii: mbc.E15-12-0844.
  PUBMED    26912792
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2016 Feb 24. pii: mbc.E15-12-0844."
COMMENT     Sequence Label: mycBioID2-13X Linker-MCS
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(582..599)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    588..699
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gctgctgaag gtgctgaaga gaatcagcga gaacctgaag aagttcaagg agaagagctt
       61 caaggagttc aagggcaaga tcgagagcaa gatgctgtac ctgggcgagg aggtgaagct
      121 gctgggcgag ggcaagatca ccggcaagct ggtgggcctg agcgagaagg gcggcgccct
      181 gatcctgacc gaggagggca tcaaggagat cctgagcggc gagttcagcc tgagaagaag
      241 caccggtttt tcggaattcg gatccggtgg aggcgggtct ggaggcgggg gtagtggcgg
      301 gggtggaagc gggggtggag gcgggtcggg tggcggaggt agcggaggcg gtggaagtgg
      361 aggcggtgga agtggtggag gaggttctgg tggcggaggt agcggtggcg gaggtagcgg
      421 aggcggtgga agtggtggcg gaggtagcgg aggcggtgga tcggcgcgcc gtttcaagaa
      481 cctcgagcgg ccgccactgt gctggatatc tgcagaattc caccacactg gactagtgga
      541 tccgagctcg gtaccaagct taagtttaaa ccgctgatca gcctcgactg tgccttctag
      601 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
      661 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
      721 ttctattctg ggggg
//
