LOCUS       sequence-181791-        8514 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Module A, Promoter: OsAct1, Gene: Csy4-P2A-TaCas9, Terminator: HSP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 8514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_A1511
FEATURES             Location/Qualifiers
     source          1..8514
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        884..1724
                     /label=Act1 promoter
                     /note="rice actin promoter"
     CDS             1727..1744
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2874..2930
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2931..7034
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2931..7034
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7035..7082
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      7094..7342
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        7422..7526
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccacct gctcatgatc tgcaggcgcg ccgtcgaggt cattcatatg
      901 cttgagaaga gagtcgggat agtccaaaat aaaacaaagg taagattacc tggtcaaaag
      961 tgaaaacatc agttaaaagg tggtataaag taaaatatcg gtaataaaag gtggcccaaa
     1021 gtgaaattta ctcttttcta ctattataaa aattgaggat gtttttgtcg gtactttgat
     1081 acgtcatttt tgtatgaatt ggtttttaag tttattcgct tttggaaatg catatctgta
     1141 tttgagtcgg gttttaagtt cgtttgcttt tgtaaataca gagggatttg tataagaaat
     1201 atctttaaaa aaacccatat gctaatttga cataattttt gagaaaaata tatattcagg
     1261 cgaattctca caatgaacaa taataagatt aaaatagctt tcccccgttg cagcgcatgg
     1321 gtattttttc tagtaaaaat aaaagataaa cttagactca aaacatttac aaaaacaacc
     1381 cctaaagttc ctaaagccca aagtgctatc cacgatccat agcaagccca gcccaaccca
     1441 acccaaccca acccacccca gtccagccaa ctggacaata gtctccacac ccccccacta
     1501 tcaccgtgag ttgtccgcac gcaccgcacg tctcgcagcc aaaaaaaaaa aaagaaagaa
     1561 aaaaaagaaa aagaaaaaac agcagggggt ccgggtcgtg ggggccggaa acgcgaggag
     1621 gatcgcgagc cagcgacgag gccggccctc cctccgcttc caaagaaacg ccccccatcg
     1681 ccactatata catacccccc ctctcctccc atccccccaa ccctaccacc accaccacca
     1741 ccacctccac ctcctccccc ctcgctgccg gacgacgagc tcctcccccc tccccctccg
     1801 ccgccgccgc gccggtaacc accccgcccc tctcctcttt ctttctccgt tttttttttc
     1861 cgtctcggtc tcgatctttg gccttggtag tttgggtggg cgagaggcgg cttcgtgcgc
     1921 gcccagatcg gtgcgcggga ggggcgggat ctcgcggctg gggctctcgc cggcgtggat
     1981 ccggcccgga tctcgcgggg aatggggctc tcggatgtag atctgcgatc cgccgttgtt
     2041 gggggagatg atggggggtt taaaatttcc gccatgctaa acaagatcag gaagagggga
     2101 aaagggcact atggtttata tttttatata tttctgctgc ttcgtcaggc ttagatgtgc
     2161 tagatctttc tttcttcttt ttgtgggtag aatttgaatc cctcagcatt gttcatcggt
     2221 agtttttctt ttcatgattt gtgacaaatg cagcctcgtg cggagctttt ttgtaggtag
     2281 acgataagct tcctgcagga agcatggacc actacctcga catcaggctc aggccagacc
     2341 cagagttccc accagcccag ctcatgtccg tcctcttcgg caagctccac caggccctcg
     2401 tggcccaggg cggcgacagg atcggcgtgt ccttcccaga cctcgacgag tccaggtcca
     2461 ggctcggcga gaggctccgc atccacgcct ccgccgacga cctcagggcc ctcctcgcca
     2521 ggccgtggct ggagggcctc agggaccacc tccagttcgg cgagccagcc gtggtgccac
     2581 acccaacccc atacaggcaa gtgtccaggg tgcaagccaa gtccaaccca gagaggctca
     2641 ggaggaggct catgaggagg cacgacctct ccgaggaaga ggccaggaag cgcatcccag
     2701 acaccgtggc cagggccctc gacctcccat tcgtgaccct caggtcccag tccaccggcc
     2761 agcacttccg cctcttcatc aggcacggcc cactccaggt gaccgccgag gagggcggct
     2821 ttacctgcta cggcctctcc aagggcggct tcgtgccgtg gttcggctcc ggcgccacca
     2881 acttctccct cctcaagcaa gccggcgacg tggaggagaa cccaggccca atggacaaga
     2941 agtactcgat cggcctcgac atcgggacga actcagttgg ctgggccgtg atcaccgacg
     3001 agtacaaggt gccctctaag aagttcaagg tcctggggaa caccgaccgc cattccatca
     3061 agaagaacct catcggcgct ctcctgttcg acagcgggga gaccgctgag gctacgaggc
     3121 tcaagagaac cgctaggcgc cggtacacga gaaggaagaa caggatctgc tacctccaag
     3181 agattttctc caacgagatg gccaaggttg acgattcatt cttccaccgc ctggaggagt
     3241 ctttcctcgt ggaggaggat aagaagcacg agcggcatcc catcttcggc aacatcgtgg
     3301 acgaggttgc ctaccacgag aagtacccta cgatctacca tctgcggaag aagctcgtgg
     3361 actccaccga taaggcggac ctcagactga tctacctcgc tctggcccac atgatcaagt
     3421 tccgcggcca tttcctgatc gagggggatc tcaacccaga caacagcgat gttgacaagc
     3481 tgttcatcca actcgtgcag acctacaacc aactcttcga ggagaacccg atcaacgcct
     3541 ctggcgtgga cgcgaaggct atcctgtccg cgaggctctc gaagtccagg aggctggaga
     3601 acctgatcgc tcagctccca ggcgagaaga agaacggcct gttcgggaac ctcatcgctc
     3661 tcagcctggg gctcaccccg aacttcaagt cgaacttcga tctcgctgag gacgccaagc
     3721 tgcaactctc caaggacacc tacgacgatg acctcgataa cctcctggcc cagatcggcg
     3781 atcaatacgc ggacctgttc ctcgctgcca agaacctgtc ggacgccatc ctcctgtcag
     3841 atatcctccg cgtgaacacc gagatcacga aggctccact ctctgcctcc atgatcaagc
     3901 gctacgacga gcaccatcag gatctgaccc tcctgaaggc gctggtccgc caacagctcc
     3961 cggagaagta caaggagatt ttcttcgatc agtcgaagaa cggctacgct gggtacatcg
     4021 acggcggggc ctcacaagag gagttctaca agttcatcaa gccaatcctg gagaagatgg
     4081 acggcacgga ggagctcctg gtgaagctca acagggagga cctcctgcgg aagcagagaa
     4141 ccttcgataa cggcagcatc ccccaccaaa tccatctcgg ggagctgcac gccatcctga
     4201 gaaggcaaga ggacttctac cctttcctca aggataaccg ggagaagatc gagaagatcc
     4261 tgaccttcag aatcccatac tacgtcggcc ctctcgcgcg ggggaactca agattcgctt
     4321 ggatgacccg caagtctgag gagaccatca cgccgtggaa cttcgaggag gtggtggaca
     4381 agggcgctag cgctcagtcg ttcatcgaga ggatgaccaa cttcgacaag aacctgccca
     4441 acgagaaggt gctccctaag cactcgctcc tgtacgagta cttcaccgtc tacaacgagc
     4501 tcacgaaggt gaagtacgtc accgagggca tgcgcaagcc agcgttcctg tccggggagc
     4561 agaagaaggc tatcgtggac ctcctgttca agaccaaccg gaaggtcacg gttaagcaac
     4621 tcaaggagga ctacttcaag aagatcgagt gcttcgattc ggtcgagatc agcggcgttg
     4681 aggaccgctt caacgccagc ctcgggacct accacgatct cctgaagatc atcaaggata
     4741 aggacttcct ggacaacgag gagaacgagg atatcctgga ggacatcgtg ctgaccctca
     4801 cgctgttcga ggacagggag atgatcgagg agcgcctgaa gacgtacgcc catctcttcg
     4861 atgacaaggt catgaagcaa ctcaagcgcc ggagatacac cggctggggg aggctgtccc
     4921 gcaagctcat caacggcatc cgggacaagc agtccgggaa gaccatcctc gacttcctca
     4981 agagcgatgg cttcgccaac aggaacttca tgcaactgat ccacgatgac agcctcacct
     5041 tcaaggagga tatccaaaag gctcaagtga gcggccaggg ggactcgctg cacgagcata
     5101 tcgcgaacct cgctggctcc cccgcgatca agaagggcat cctccagacc gtgaaggttg
     5161 tggacgagct cgtgaaggtc atgggccggc acaagcctga gaacatcgtc atcgagatgg
     5221 ccagagagaa ccaaaccacg cagaaggggc aaaagaactc tagggagcgc atgaagcgca
     5281 tcgaggaggg catcaaggag ctggggtccc aaatcctcaa ggagcaccca gtggagaaca
     5341 cccaactgca gaacgagaag ctctacctgt actacctcca gaacggcagg gatatgtacg
     5401 tggaccaaga gctggatatc aaccgcctca gcgattacga cgtcgatcat atcgttcccc
     5461 agtctttcct gaaggatgac tccatcgaca acaaggtcct caccaggtcg gacaagaacc
     5521 gcggcaagtc agataacgtt ccatctgagg aggtcgttaa gaagatgaag aactactgga
     5581 ggcagctcct gaacgccaag ctgatcacgc aaaggaagtt cgacaacctc accaaggctg
     5641 agagaggcgg gctctcagag ctggacaagg ccggcttcat caagcggcag ctggtcgaga
     5701 ccagacaaat cacgaagcac gttgcgcaaa tcctcgactc tcggatgaac acgaagtacg
     5761 atgagaacga caagctgatc agggaggtta aggtgatcac cctgaagtct aagctcgtct
     5821 ccgacttcag gaaggatttc cagttctaca aggttcgcga gatcaacaac taccaccatg
     5881 cccatgacgc ttacctcaac gctgtggtcg gcaccgctct gatcaagaag tacccaaagc
     5941 tggagtccga gttcgtgtac ggggactaca aggtttacga tgtgcgcaag atgatcgcca
     6001 agtcggagca agagatcggc aaggctaccg ccaagtactt cttctactca aacatcatga
     6061 acttcttcaa gaccgagatc acgctggcca acggcgagat ccggaagaga ccgctcatcg
     6121 agaccaacgg cgagacgggg gagatcgtgt gggacaaggg cagggatttc gcgaccgtcc
     6181 gcaaggttct ctccatgccc caggtgaaca tcgtcaagaa gaccgaggtc caaacgggcg
     6241 ggttctcaaa ggagtctatc ctgcctaagc ggaacagcga caagctcatc gccagaaaga
     6301 aggactggga cccaaagaag tacggcgggt tcgacagccc taccgtggcc tactcggtcc
     6361 tggttgtggc gaaggttgag aagggcaagt ccaagaagct caagagcgtg aaggagctcc
     6421 tggggatcac catcatggag aggtccagct tcgagaagaa cccaatcgac ttcctggagg
     6481 ccaagggcta caaggaggtg aagaaggacc tgatcatcaa gctcccgaag tactctctct
     6541 tcgagctgga gaacggcagg aagagaatgc tggcttccgc tggcgagctc cagaagggga
     6601 acgagctcgc gctgccaagc aagtacgtga acttcctcta cctggcttcc cactacgaga
     6661 agctcaaggg cagcccggag gacaacgagc aaaagcagct gttcgtcgag cagcacaagc
     6721 attacctcga cgagatcatc gagcaaatct ccgagttcag caagcgcgtg atcctcgccg
     6781 acgcgaacct ggataaggtc ctctccgcct acaacaagca ccgggacaag cccatcagag
     6841 agcaagcgga gaacatcatc catctcttca ccctgacgaa cctcggcgct cctgctgctt
     6901 tcaagtactt cgacaccacg atcgatcgga agagatacac ctccacgaag gaggtcctgg
     6961 acgcgaccct catccaccag tcgatcaccg gcctgtacga gacgaggatc gacctctcac
     7021 aactcggcgg ggataagaga cccgcagcaa ccaagaaggc agggcaagca aagaagaaga
     7081 agtgactcga gtatatgaag atgaagatga aatatttggt gtgtcaaata aaaagcttgt
     7141 gtgcttaagt ttgtgttttt ttcttggctt gttgtgttat gaatttgtgg ctttttctaa
     7201 tattaaatga atgtaagatc acattataat gaataaacaa atgtttctat aatccattgt
     7261 gaatgttttg ttggatctct tctgcagcat ataactactg tatgtgctat ggtatggact
     7321 atggaatatg attaaagata aggagctcgt taaccggtga cggacgtggg caggtgtgat
     7381 gaaaagcctt agggtcaggt ggcacttttc ggggaaatgt gcgatgaacc cctatttgtt
     7441 tatttttcta aatacattca aatatgtatc cgctaatgag acaataaccc tgataaatgc
     7501 ttcaattata ttgaaaaagg aagagtatga gtatccaaca tttccgtgtc gcccttattc
     7561 ccttttttgc ggccttctgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     7621 aagatgctga agatcagttg ggtgcccgag tgggttacat tgaactggat ctcaacagcg
     7681 gcaaaatctt agagagtttt cgccccgaag aacgcttccc aatgatgagc actttcaaag
     7741 ttctgctatg tggcgcggtg ttatcccgta ttgatgctgg gcaagagcaa ctcggtcgcc
     7801 gcatacacta ttctcagaat gacttggttg agtattcacc agtcacagaa aagcatctta
     7861 cggatggcat gacagtaaga gaattatgta gtgctgccat aaccatgagt gataacactg
     7921 cggccaactt acttctgaca acgattggag gaccgaagga gctaaccgct tttttgcaca
     7981 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     8041 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaactctc cgcaaactat
     8101 taactggcga actacttact cttgcttcac gccagcaact cattgactgg atggaggcgg
     8161 acaaagttgc aggaccactt ctgcgctcgg cacttccggc tggctggttt attgctgata
     8221 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     8281 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     8341 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagacgaag
     8401 ggaaataaat agtagcccgc ctgatgtgcg ggcttttttt tggtggtggt taccagtgtc
     8461 gcttagctac cgtaggtcct cttaggcggt ccgattagcg acatagtcat caat
//
