LOCUS       sequence-181799-        9240 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module A, Promoter: ZmUbi, Gene: TREX2-P2A-TaCas9, Terminator: HSP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9240)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 9240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_A1910
FEATURES             Location/Qualifiers
     source          1..9240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             3600..3656
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3657..7760
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3657..7760
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7761..7808
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     promoter        8148..8252
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccacct gctcatgatc tgcaggcgcg cctgcagtgc agcgtgaccc
      901 ggtcgtgccc ctctctagag ataatgagca ttgcatgtct aagttataaa aaattaccac
      961 atattttttt tgtcacactt gtttgaagtg cagtttatct atctttatac atatatttaa
     1021 actttactct acgaataata taatctatag tactacaata atatcagtgt tttagagaat
     1081 catataaatg aacagttaga catggtctaa aggacaattg agtattttga caacaggact
     1141 ctacagtttt atctttttag tgtgcatgtg ttctcctttt tttttgcaaa tagcttcacc
     1201 tatataatac ttcatccatt ttattagtac atccatttag ggtttagggt taatggtttt
     1261 tatagactaa tttttttagt acatctattt tattctattt tagcctctaa attaagaaaa
     1321 ctaaaactct attttagttt ttttatttaa taatttagat ataaaataga ataaaataaa
     1381 gtgactaaaa attaaacaaa taccctttaa gaaattaaaa aaactaagga aacatttttc
     1441 ttgtttcgag tagataatgc cagcctgtta aacgccgtcg acgagtctaa cggacaccaa
     1501 ccagcgaacc agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtcg
     1561 ctgcctctgg acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca
     1621 tccagaaatt gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc
     1681 ctctcacggc accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc
     1741 ctcgcccgcc gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttgt
     1801 tcggagcgca cacacacaca accagatctc ccccaaatcc acccgtcggc acctccgctt
     1861 caaggtacgc cgctcgtcct cccccccccc ctctctacct tctctagatc ggcgttccgg
     1921 tccatggtta gggcccggta gttctacttc tgttcatgtt tgtgttagat ccgtgtttgt
     1981 gttagatccg tgctgctagc gttcgtacac ggatgcgacc tgtacgtcag acacgttctg
     2041 attgctaact tgccagtgtt tctctttggg gaatcctggg atggctctag ccgttccgca
     2101 gacgggatcg atttcatgat tttttttgtt tcgttgcata gggtttggtt tgcccttttc
     2161 ctttatttca atatatgccg tgcacttgtt tgtcgggtca tcttttcatg cttttttttg
     2221 tcttggttgt gatgatgtgg tctggttggg cggtcgttct agatcggagt agaattaatt
     2281 ctgtttcaaa ctacctggtg gatttattaa ttttggatct gtatgtgtgt gccatacata
     2341 ttcatagtta cgaattgaag atgatggatg gaaatatcga tctaggatag gtatacatgt
     2401 tgatgcgggt tttactgatg catatacaga gatgcttttt gttcgcttgg ttgtgatgat
     2461 gtggtgtggt tgggcggtcg ttcattcgtt ctagatcgga gtagaatact gtttcaaact
     2521 acctggtgta tttattaatt ttggaactgt atgtgtgtgt catacatctt catagttacg
     2581 agtttaagat ggatggaaat atcgatctag gataggtata catgttgatg tgggttttac
     2641 tgatgcatat acatgatggc atatgcagca tctattcata tgctctaacc ttgagtacct
     2701 atctattata ataaacaagt atgttttata attattttga tcttgatata cttggatgat
     2761 ggcatatgca gcagctatat gtggattttt ttagccctgc cttcatacgc tatttatttg
     2821 cttggtactg tttcttttgt cgatgctcac cctgttgttt ggtgttactt cctgcaggaa
     2881 gcatgtctga gccacctcgg gctgagacct ttgtattcct ggacctagaa gccactgggc
     2941 tcccaaacat ggaccctgag attgcagaga tatccctttt tgctgttcac cgctcttccc
     3001 tggagaaccc agaacgggat gattctggtt ccttggtgct gccccgtgtt ctggacaagc
     3061 tcacactgtg catgtgcccg gagcgcccct ttactgccaa ggccagtgag attactggtt
     3121 tgagcagcga aagcctgatg cactgcggga aggctggttt caatggcgct gtggtaagga
     3181 cactgcaggg cttcctaagc cgccaggagg gccccatctg ccttgtggcc cacaatggct
     3241 tcgattatga cttcccactg ctgtgcacgg agctacaacg tctgggtgcc catctgcccc
     3301 aagacactgt ctgcctggac acactgcctg cattgcgggg cctggaccgt gctcacagcc
     3361 acggcaccag ggctcaaggc cgcaaaagct acagcctggc cagtctcttc caccgctact
     3421 tccaggctga acccagtgct gcccattcag cagaaggtga tgtgcacacc ctgcttctga
     3481 tcttcctgca tcgtgctcct gagctgctcg cctgggcaga tgagcaggcc cgcagctggg
     3541 ctcatattga gcccatgtac gtgccacctg atggtccaag cctcgaagcc ggctccggcg
     3601 ccaccaactt ctccctcctc aagcaagccg gcgacgtgga ggagaaccca ggcccaatgg
     3661 acaagaagta ctcgatcggc ctcgacatcg ggacgaactc agttggctgg gccgtgatca
     3721 ccgacgagta caaggtgccc tctaagaagt tcaaggtcct ggggaacacc gaccgccatt
     3781 ccatcaagaa gaacctcatc ggcgctctcc tgttcgacag cggggagacc gctgaggcta
     3841 cgaggctcaa gagaaccgct aggcgccggt acacgagaag gaagaacagg atctgctacc
     3901 tccaagagat tttctccaac gagatggcca aggttgacga ttcattcttc caccgcctgg
     3961 aggagtcttt cctcgtggag gaggataaga agcacgagcg gcatcccatc ttcggcaaca
     4021 tcgtggacga ggttgcctac cacgagaagt accctacgat ctaccatctg cggaagaagc
     4081 tcgtggactc caccgataag gcggacctca gactgatcta cctcgctctg gcccacatga
     4141 tcaagttccg cggccatttc ctgatcgagg gggatctcaa cccagacaac agcgatgttg
     4201 acaagctgtt catccaactc gtgcagacct acaaccaact cttcgaggag aacccgatca
     4261 acgcctctgg cgtggacgcg aaggctatcc tgtccgcgag gctctcgaag tccaggaggc
     4321 tggagaacct gatcgctcag ctcccaggcg agaagaagaa cggcctgttc gggaacctca
     4381 tcgctctcag cctggggctc accccgaact tcaagtcgaa cttcgatctc gctgaggacg
     4441 ccaagctgca actctccaag gacacctacg acgatgacct cgataacctc ctggcccaga
     4501 tcggcgatca atacgcggac ctgttcctcg ctgccaagaa cctgtcggac gccatcctcc
     4561 tgtcagatat cctccgcgtg aacaccgaga tcacgaaggc tccactctct gcctccatga
     4621 tcaagcgcta cgacgagcac catcaggatc tgaccctcct gaaggcgctg gtccgccaac
     4681 agctcccgga gaagtacaag gagattttct tcgatcagtc gaagaacggc tacgctgggt
     4741 acatcgacgg cggggcctca caagaggagt tctacaagtt catcaagcca atcctggaga
     4801 agatggacgg cacggaggag ctcctggtga agctcaacag ggaggacctc ctgcggaagc
     4861 agagaacctt cgataacggc agcatccccc accaaatcca tctcggggag ctgcacgcca
     4921 tcctgagaag gcaagaggac ttctaccctt tcctcaagga taaccgggag aagatcgaga
     4981 agatcctgac cttcagaatc ccatactacg tcggccctct cgcgcggggg aactcaagat
     5041 tcgcttggat gacccgcaag tctgaggaga ccatcacgcc gtggaacttc gaggaggtgg
     5101 tggacaaggg cgctagcgct cagtcgttca tcgagaggat gaccaacttc gacaagaacc
     5161 tgcccaacga gaaggtgctc cctaagcact cgctcctgta cgagtacttc accgtctaca
     5221 acgagctcac gaaggtgaag tacgtcaccg agggcatgcg caagccagcg ttcctgtccg
     5281 gggagcagaa gaaggctatc gtggacctcc tgttcaagac caaccggaag gtcacggtta
     5341 agcaactcaa ggaggactac ttcaagaaga tcgagtgctt cgattcggtc gagatcagcg
     5401 gcgttgagga ccgcttcaac gccagcctcg ggacctacca cgatctcctg aagatcatca
     5461 aggataagga cttcctggac aacgaggaga acgaggatat cctggaggac atcgtgctga
     5521 ccctcacgct gttcgaggac agggagatga tcgaggagcg cctgaagacg tacgcccatc
     5581 tcttcgatga caaggtcatg aagcaactca agcgccggag atacaccggc tgggggaggc
     5641 tgtcccgcaa gctcatcaac ggcatccggg acaagcagtc cgggaagacc atcctcgact
     5701 tcctcaagag cgatggcttc gccaacagga acttcatgca actgatccac gatgacagcc
     5761 tcaccttcaa ggaggatatc caaaaggctc aagtgagcgg ccagggggac tcgctgcacg
     5821 agcatatcgc gaacctcgct ggctcccccg cgatcaagaa gggcatcctc cagaccgtga
     5881 aggttgtgga cgagctcgtg aaggtcatgg gccggcacaa gcctgagaac atcgtcatcg
     5941 agatggccag agagaaccaa accacgcaga aggggcaaaa gaactctagg gagcgcatga
     6001 agcgcatcga ggagggcatc aaggagctgg ggtcccaaat cctcaaggag cacccagtgg
     6061 agaacaccca actgcagaac gagaagctct acctgtacta cctccagaac ggcagggata
     6121 tgtacgtgga ccaagagctg gatatcaacc gcctcagcga ttacgacgtc gatcatatcg
     6181 ttccccagtc tttcctgaag gatgactcca tcgacaacaa ggtcctcacc aggtcggaca
     6241 agaaccgcgg caagtcagat aacgttccat ctgaggaggt cgttaagaag atgaagaact
     6301 actggaggca gctcctgaac gccaagctga tcacgcaaag gaagttcgac aacctcacca
     6361 aggctgagag aggcgggctc tcagagctgg acaaggccgg cttcatcaag cggcagctgg
     6421 tcgagaccag acaaatcacg aagcacgttg cgcaaatcct cgactctcgg atgaacacga
     6481 agtacgatga gaacgacaag ctgatcaggg aggttaaggt gatcaccctg aagtctaagc
     6541 tcgtctccga cttcaggaag gatttccagt tctacaaggt tcgcgagatc aacaactacc
     6601 accatgccca tgacgcttac ctcaacgctg tggtcggcac cgctctgatc aagaagtacc
     6661 caaagctgga gtccgagttc gtgtacgggg actacaaggt ttacgatgtg cgcaagatga
     6721 tcgccaagtc ggagcaagag atcggcaagg ctaccgccaa gtacttcttc tactcaaaca
     6781 tcatgaactt cttcaagacc gagatcacgc tggccaacgg cgagatccgg aagagaccgc
     6841 tcatcgagac caacggcgag acgggggaga tcgtgtggga caagggcagg gatttcgcga
     6901 ccgtccgcaa ggttctctcc atgccccagg tgaacatcgt caagaagacc gaggtccaaa
     6961 cgggcgggtt ctcaaaggag tctatcctgc ctaagcggaa cagcgacaag ctcatcgcca
     7021 gaaagaagga ctgggaccca aagaagtacg gcgggttcga cagccctacc gtggcctact
     7081 cggtcctggt tgtggcgaag gttgagaagg gcaagtccaa gaagctcaag agcgtgaagg
     7141 agctcctggg gatcaccatc atggagaggt ccagcttcga gaagaaccca atcgacttcc
     7201 tggaggccaa gggctacaag gaggtgaaga aggacctgat catcaagctc ccgaagtact
     7261 ctctcttcga gctggagaac ggcaggaaga gaatgctggc ttccgctggc gagctccaga
     7321 aggggaacga gctcgcgctg ccaagcaagt acgtgaactt cctctacctg gcttcccact
     7381 acgagaagct caagggcagc ccggaggaca acgagcaaaa gcagctgttc gtcgagcagc
     7441 acaagcatta cctcgacgag atcatcgagc aaatctccga gttcagcaag cgcgtgatcc
     7501 tcgccgacgc gaacctggat aaggtcctct ccgcctacaa caagcaccgg gacaagccca
     7561 tcagagagca agcggagaac atcatccatc tcttcaccct gacgaacctc ggcgctcctg
     7621 ctgctttcaa gtacttcgac accacgatcg atcggaagag atacacctcc acgaaggagg
     7681 tcctggacgc gaccctcatc caccagtcga tcaccggcct gtacgagacg aggatcgacc
     7741 tctcacaact cggcggggat aagagacccg cagcaaccaa gaaggcaggg caagcaaaga
     7801 agaagaagtg actcgagtat atgaagatga atatgaaata tttggtgtgt caaataaaaa
     7861 gcttgtgtgc ttaagtttgt gtttttttct tggcttgttg tgttatgaat ttgtggcttt
     7921 ttctaatatt aaatgaatgt aagatcacat tataatgaat aaacaaatgt ttctataatc
     7981 cattgtgaat gttttgttgg atctcttctg cagcatataa ctactgtatg tgctatggta
     8041 tggactatgg aatatgatta aagataagga gctcgttaac cggtgacgga cgtgggcagg
     8101 tgtgatgaaa agccttaggg tcaggtggca cttttcgggg aaatgtgcga tgaaccccta
     8161 tttgtttatt tttctaaata cattcaaata tgtatccgct aatgagacaa taaccctgat
     8221 aaatgcttca attatattga aaaaggaaga gtatgagtat ccaacatttc cgtgtcgccc
     8281 ttattccctt ttttgcggcc ttctgccttc ctgtttttgc tcacccagaa acgctggtga
     8341 aagtaaaaga tgctgaagat cagttgggtg cccgagtggg ttacattgaa ctggatctca
     8401 acagcggcaa aatcttagag agttttcgcc ccgaagaacg cttcccaatg atgagcactt
     8461 tcaaagttct gctatgtggc gcggtgttat cccgtattga tgctgggcaa gagcaactcg
     8521 gtcgccgcat acactattct cagaatgact tggttgagta ttcaccagtc acagaaaagc
     8581 atcttacgga tggcatgaca gtaagagaat tatgtagtgc tgccataacc atgagtgata
     8641 acactgcggc caacttactt ctgacaacga ttggaggacc gaaggagcta accgcttttt
     8701 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     8761 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca actctccgca
     8821 aactattaac tggcgaacta cttactcttg cttcacgcca gcaactcatt gactggatgg
     8881 aggcggacaa agttgcagga ccacttctgc gctcggcact tccggctggc tggtttattg
     8941 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     9001 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     9061 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     9121 acgaagggaa ataaatagta gcccgcctga tgtgcgggct tttttttggt ggtggttacc
     9181 agtgtcgctt agctaccgta ggtcctctta ggcggtccga ttagcgacat agtcatcaat
//
